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Cupressus Linn., commonly known as ‘cypress', belongs to the family Cupressaceae. It consists of 20 species with a wide and discontinuous distribution in the Northern Hemisphere. In the present study, three species, namely C. torulosa Don., C. arizonica Greene and C. glabra Sudw., growing in the western Himalayan region of India at an altitude of 1760 m. have been studied. This is a preliminary attempt where embryology is used in estimating seed quality and in seed testing. Seed quality was analysed according to five (0-IV) seed classes based on the presence of the embryo and endosperm and their nature of development in the seed-cutting test. Seeds having neither embryo nor endosperm belong to class ‘0'. In class ‘I' seeds contain the endosperm but a shrivelled embryo or sometimes no embryo. Class ‘II' contains seeds with an embryo of half-length of the embryo cavity and class ‘III' seeds containing an embryo of at least three-quarter length of the embryo cavity. Seeds with completely developed full embryo were assigned to class ‘IV'. However a high percentage of seeds of classes 0-III present in seed lots from particular trees of a species decrease the quality of seeds. Similarly a high percentage of seeds of class ‘IV' improves the seed quality of a given tree selected as plus tree for germplasm collection.
In the years 1996-1997 the experiments were carried out on methods to investigate seed vigour of tassel flower (Amaranthus caudatus L.), sand pink (Dianthus chinensis L.), babies' breath (Gypsophila elegans M.R), sweet pea (Lathyrus odorathus L.), African marigold (Tagetes erecta L.) and zinnia (Zinnia elegans Jasq.). The main goals of this research were to specify conditions for accelerated ageing (AA) of the seeds of a few selected ornamental plant species and to choose the most appropriate methods for their seed vigour evaluation in the laboratory and greenhouse conditions. All used in the experiments seeds came from the commercial seed lots from Polish seed company. Evaluation was carried out on the seed samples with high and low vigour. The latter ones were received through subjecting the seed samples to AA, i.e. by placing them in 100% relative humidity (RH) at 44°C, except African marigold - at 42°C, in the darkness and keeping them for 144, 88, 100,48,72 and 72 hours, respectively. The tested seed vigour estimated methods included the Germ' s method, the 2,3,5-triphenyl tetrazoilum chloride (TTC) method and the test of plant emergences in the greenhouse. The high vigour seeds sampies were used as a check. The Germ' s method was found to be useful to evaluate sand pink, babies' breath and African marigold seed vigour, whereas the TTC method was found to be suitable for vigour evaluation of sand pink, babies' breath and zinnia. At present stage of our knowledge about seed vigour, the plant emergences in the greenhouse method was found to be the best for evaluation of seed vigour of tassel flower, sand pink, babies' breath, sweet pea and zinnia. It is reasonable to combine a few methods of seed vigour evaluation for ornamental plant species.
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