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Woodpeckers feed primarily on insects, larvae and other arthropods; however, several members of this family include plant products in their diets, such as sap. Among them, the genera Sphyrapicus and Melanerpes include the most species that specialize in sap consumption. In semiarid forests of Argentina, sap is an important food item in the diet of the White-fronted Woodpecker, Melanerpes cactorum. The aim of this study is to investigate why White-fronted Woodpeckers only consume sap from certain plants while avoiding other available plants of the same species and explore seasonality of their plant selection. We expected that combinations of plant traits (i.e. sugars concentration of sap, sap flow intensity, plant size, plant health and plant microhabitat), rather than one particular trait, determine which tree they select for sap feeding in different seasons. We examined five plant species: Sarcotoxicum salicifolium, Prosopis ruscifolia, Ziziphus mistol, Aspidosperma quebracho-bianco and Stetsonia coryne that were used most frequently for sap consumption and were consumed in all seasons by ten groups of White-fronted Woodpecker in semiarid Chaco, Argentina. Plants selected by White-fronted Woodpeckers for sap consumption were mainly larger plants that yield high sugar concentration. Of the plant species we studied, individual plant selection in all seasons was more evident in those plant species that constitute an important part of their diet (i.e. Prosopis ruscifolia and Stetsonia coryne). The selection of plants offering a greater reward in sap quality strongly suggests that the White-fronted Woodpecker maximizes food energy intake as a response to the seasonality that characterizes semiarid climates of temperate regions and conditions of food resources availability. Our results show that large trees are selected as sap trees by White-fronted Woodpecker, therefore, we recommend activities that promote retention of large trees in Chaco region.
In the present study, we described the protein profile experimentally by 2D-PAGE and MALDI analysis to understand the stress mechanisms of cocoti sap and wine on E.coli Nissle 1917. We isolated one newly expressed protein from cocoti wine treated gel which is not present in both control and cocoti sap treated sample i.e. P21 prophage-derived head-stabilizing proteinVG03_ECOL6 (3n1) also called as Head protein gp3. This protein mainly activities related to the viral life cycle. It helps to attach the viral gene into host. The growth rate was delayed in cocoti wine treated E.coli Nissle 1917 when compared to control and cocoti sap treated samples. Stress mechanism induce many proteins they are involved in metabolic process, hydrolase activity, lyase activity, quinone binding, phosphotransferase system, carbohydrate metabolism, DNA binding, DNA repair, transferase activity, oxidoreductase, purine metabolism, transcription antitermination, transcription regulation and other related activities. We proved that the predicted protein structure quality, resolution, density and error plot values by QMEAN analysis. Based on these results, only two differentially expressed proteins under sap stress showed that the significant results, which were N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1, PTPB1_ECOLI and DinI-like protein Z3305/ECs2939 in prophage CP-933VDINI1_ECO57. In case of wine stress, the differentially expressed proteins were Transcription anti-termination protein RFAH- ECO57 NusA and PUR7- eco24- phosphoribosylamidazole-succinocarboxamide synthase showed significant results. ProtParam analysis indicating that the multiple physico-chemical characters of differentially expressed proteins were differed and compared. The phylogenetic tree represents the relationship in-between the differentially expressed proteins, were showed siblings (related) as well as monophytic clade.
Improved soil fertility is a requirement for enhanced crop production. Combination of inorganic and organic fertilizers improve crop productivity while reduce environmental degradation. An experiment was carried out at Eastern University, Sri Lanka to study the effect of Foliar Application of Banana Pseudostem Sap on Yield of Cowpea (Vigna unguiculata L. Walp.) in sandy regosol. The experiment was laid out in Randomized Complete Block Design having five treatments viz; recommended inorganic fertilizer N, P, K as basal and N as topdressing (T1), N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% , 3%, 5% and 7% banana pseudostem sap solution (T2-T5) at 3rd, 5th, 7th and 9th week after planting. The results revealed that significant difference (P<0.05) were noted on number of pods per plant at 1st and 2nd picking. Higher number of pods per plant, maximum pod length and girth were in T2 while low in T1. Sun dried weights of pods and number of seeds per pod were significantly (P<0.05) varied at each picking. Further, cowpea yield at each picking were higher in T2 compared to tested treatments. The present study suggested that, among the tested treatments N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% Pseudostem sap solution at 3rd, 5th, 7th and 9th WAP would be the most suitable for cowpea production in sandy regosol.
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