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This study was conducted to determine the effects of Bacillus licheniformis (Bl) and Clostridium butyricum (Cb) and their combinations with yeast culture on in vitro rumen fermentation in a two-way factorial design. Treatments included Bl or Cb at levels of 0, 0.5, 1, 5 and 10 mg and their combination with yeast culture at 0, 18, 27, 36 and 60 mg per 200 mg substrate, respectively. Gas production was recorded after 2, 4, 6, 9, 12, 24, 36, 48 and 72 h incubation. In vitro organic matter digestibility (IVOMD) was estimated by 24 h gas production. Rumen fermentation parameters were determined after 24 h of incubation. Rate constant of gas production was not influenced by Bl or Cb alone, but increased (P<0.05) with inclusion of yeast culture. The IVOMD was influenced (P<0.05) by addition with Bl, Cb or yeast culture, with highest IVOMD observed when Bl or Cb was combined with 60 mg yeast culture. Total volatile fatty acids were affected by Bl and yeast culture (P<0.01), but not by Cb (P>0.05). There were significant interaction effects on pH, acetate to propionate ratio and ammonia-N between yeast culture and Bl or Cb. From the above results, it is indicated that Bl and Cb may be more effective as feed additives when combined with yeast culture than when offered separately.
The purpose of the study was to determine the effect of a silage additive on maize silage fermentation and assess the relationship of silages with different levels of fermentation end-products to ruminal fermentation parameters and organic matter (OM) digestibility. The maize silage underwent two treatments: a) no additive (control silage), b) additive with Lactobacillus plantarum and Pediococcus acidilacticci and enzyme cellulase. The additive improved silage fermentative processes, preserved more water soluble carbohydrates, increased fermentation of lactic acid and decreased formation of ammonia-N. Two groups of analogous cows were formed: the cows (n = 10) of the control group were fed silage without additives, the cows (n = 10) of the experimental group the silage additive. Rumen fluid from cows was sampled. The silage additive improved fermentation processes in the rumen: the amount of volatile fatty acids, including propionic acid, increased as well as the total and lactate fermenting bacterial count and number of protozoa. The incubation of both grass hay and also silage with the rumen fluid from the cows receiving silage with inoculant and cellulase additive improved their OM digestibility.
Two trials were conducted to investigate the effect of a combination of essential oils (CEO) along with fumarate on in vitro rumen fermentation. In trial 1, the essential oil (EO) from thyme, oregano, cinnamon and lemon were mixed at five different ratios. The CEO were applied at levels of 0-500 mg/l. Addition of CEO decreased gas, methane, total volatile fatty acid (VFA) production at 24 h incubation in a dose-dependent manner. Methane tended to decrease much more than total VFA and gas at the same EO level. The mixture of oils at an equal ratio at 500 mg/l that decreased methane much more than VFA was chosen as the optimal combination. In trial 2, the optimal combination was used with 0, 5, 10 or 15 mmol/l of monosodium fumarate. Addition of fumarate further decreased methane production, with 10 mmol/l fumarate resulting in the largest reduction (80.2%) and the smallest decrease in total VFA (5.7%) and gas production (16.7%). Quantification of several ruminal microbe populations by RT-PCR showed that the optimal combination sharply decreased ruminal protozoa; the populations of fungi and fibrolytic bacteria were also decreased. In summary, at an appropriate level, CEO can inhibit methane production. Inclusion of fumarate can further decrease it, which is attributed mainly to inhibition of protozoa and methanogens.
This study evaluated the effects of addition of lucerne extract (LE), Artemisiae annuae extract (AAE), and mixed herbal medicine (MHM) into different goat diets on in vitro rumen fermentation and methane production. In addition to the negative control (NC), addition of monensin (MO) served as the positive control (PC). Four ruminally cannulated Nanjiang Yellow goats (45 ± 2 kg liveweight) were used as donors of ruminal fluid. The results showed: 1. compared with NC, addition of all the additives into the mixed grass diet increased propionate concentration (P<0.05). Protozoa numbers and methane production were reduced by addition of all the additives (P<0.05); 2. compared with NC, LE and AAE in the lucerne diet increased propionate concentration (P<0.05). All the additives reduced protozoa numbers (P<0.05). Methane production was decreased by addition of LE compared with NC and PC (P<0.05); 3. addition of LE into the mixed grass-concentrate diet resulted in an increase of the propionate concentration compared with NC and PC (P<0.05). Protozoa numbers were reduced by addition of LE and AAE compared with NC (P<0.05). Methane production was decreased by addition of AAE compared with NC and PC (P<0.05); 4. compared with NC, addition of AAE and MHM into the lucerne-concentrate diet resulted in an increase of propionate concentration (P<0.05). Protozoa numbers were reduced by addition of all the additives (P<0.05). Methane production was decreased by addition of AAE and MHM (P<0.05). In conclusion, addition of LE, AAE and MHM into different diets reduced methane production, increased propionate concentration and decreased protozoa numbers to a certain extent, and the inhibitory effects of the phytogenic products on methane production are more remarkable in the mixed-grass diet. The phytogenic products appear to be promising alternatives to MO in altering in vitro rumen fermentation and reducing methane production in goats.
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