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A natural rubber degrading candidate was isolated from a soil sample from Aswan, Egypt. The strain was able to grow on natural rubber as a sole source for carbon and energy. According to its degradation behavior, it grew adhesively and in direct contact with the rubber substrate and led to disintegration of the material during cultivation. Furthermore, this strain was not able to form a clear zone (translucent halos) around bacterial colonies after cultivation on NR latex plates. Taxo-nomic analysis of the strain based on partial 16S rRNA similarity examinations indicated that bacterial candidate belongs to genus Achromobacter sp. Schiff's reagent staining tests performed during cultivation of the strain on NR latex gloves of different sizes, treated or nontreated, revealed that the strain was able to colonize the rubber surface. Formation of bacterial films and occurrence of compounds containing aldehyde groups during cultivation was observed. The tested strain showed a higher colonization efficiency on small or treated pieces of NR latex gloves, while a lower colonization efficiency was recognized when grown on large or nontreated NR latex gloves. Plackett-Burman experimental design, based on numerical modeling, was applied to evaluate the significance of culture conditions affecting natural rubber degradation by the bacterial candidate. Eleven variables through fourteen trials were studied simultaneously. Based on rubber mineralization data, the highest positive variables affecting rubber degradation were NR granules, K₂HPO₄ Na-succinate and NH₄Cl, while MgSO₄ x 7H₂O and KH₂PO₄ were the lowest significant variables.
Phenylalanine ammonia lyase (PAL) is a specific branch point enzyme of primary and secondary metabolism. It plays a key role in plant development and defense mechanisms. Phenylalanine ammonia lyase from Hevea brasiliensis (HbPAL) presented a complete open reading frame (ORF) of 2,145 bp with 721 encoded amino acids. The sequence alignment indicated that the amino acid sequence of HbPAL shared a high identity with PAL genes found in other plants. Phylogenetic tree analysis indicated that HbPAL was more closely related to PALs in Manihot esculenta and Jatropha curcas than to those from other plants. Transcription pattern analysis indicated that HbPAL was constitutively expressed in all tissues examined, most highly in young leaves. The HbPAL gene was evaluated by quantitative real-time PCR (qRT-PCR) after infection with Rigidoporus microporus at 0, 12, 24, 48, 72 and 96 hours post inoculation. The expression patterns of the PAL gene differed among the three rubber clones used in the study. The transcription level of the white root rot disease tolerant clone, PB5/51 increased sharply during the latter stages of infection, while it was relatively subdued in the white root rot disease susceptible clones, RRIM600 and BPM24. These results suggest that the HbPAL gene may play a role in the molecular defense response of H. brasiliensis to pathogen attack and could be used as a selection criterion for disease tolerance.
The paper attempts to assess the extent of crop loss in rubber plantations in India, measured in terms of loss in latex and timber output and thereby to examine the comparative economics of plant protection measures against Phytophtora spp. induced abnormal leaf fall (ALF). The specific objectives were: a) to examine the extent of loss in latex and timber output in unsprayed plots vis-a-vis sprayed plots across prominent rubber clones; b) estimate the value of loss in latex and timber output across clones between sprayed and unsprayed plots; c) examine the comparative economics of plant protection measures in terms of the incremental costs and the incremental returns from sprayed plots across clones; and d) reflect upon the policy imperatives with respect to region-specific Research and Development (R&D) interventions on plant protection measures in India. The study brings out significant clonal differences in loss of latex and timber output in the absence of prophylactic spraying against ALF. The observed clonal differences with respect to feasibility of plant disease control measures indicate the need for region and clone-specific recommendations for plant protection measures in India instead of the currently followed unilateral prescription with due allowance to the costs and potential benefit accrued from the control measures. The study also highlight the need for evolving interventions and agro-management/ plant protection measures for minimising the incidence of tree casualty in rubber plantation, as it amounts to loss of potential income from latex and timber from rubber plantations in India, dominated by the smallholder sector.
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