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Application of multiplex PCR in routine microbiological diagnostics of Listeria monocytogenes and Listeria sp. strains in a meat-processing plant

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Medrala D., Dabrowski W., Szymanska L.

Polish Journal of Food and Nutrition Sciences

2003

tom 12/53

nr 1

A multiplex PCR assay designed by Bansal [1996] was applied in a routine screening test of a meat-processing environment aimed at detection of Listeria monocytogenes and species of the genus Listeria. Listeria spp. strains yielded a single 938-bp product indicating presence of 16S rRNA conservative sequence typical of the genus, whereas L. monocytogenes strains yielded not only the 938-bp product but also a 750-bp product - a result of amplification within region of the listeriolysin (hly A) gene. The assay was used to verify identification of 50 colonies performed using classical tests, including catalase and hemolytic activity, motility and API®LISTERIA (bioMérieux) biochemical tests. Among isolates, 4 strains (8%) were found characterised by contradictory results of biochemical and genetic tests. The possibility of simultaneous identification and differentiation of potentially pathogenic L. monocytogenes strains from other Listeria spp. during one reaction performed was proved.

Ograniczanie wyników

1 Polish Journal of Food and Nutrition Sciences

1 Dabrowski W.

1 Medrala D.

1 Szymanska L.

1 2003

Wyniki wyszukiwania

Application of multiplex PCR in routine microbiological diagnostics of Listeria monocytogenes and Listeria sp. strains in a meat-processing plant