A quantitative HPLC analysis of rosmarinic acid has been carried out in different samples of lemon balm leaves. The contents of rosmarinic acid in the samples varied significantly, ranging from 0.9% to 2.79%. Similar differentiation has been found in the content of total phenolic acids (4.08%- -11.93%) determined by means of spectrophotometric method, and also in the content of tannins (3.6%-13.2%) determined using the method with skin powder. The percentages of total flavonoids estimated by spectrophotometric method were within the limit 0.32%-0.44%. The HPLC estimation of rosmarinic acid or spectrophotometric estimation of the total phenolic acids should be regarded for the standardisation of the Melissae folium.
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