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The present investigation was carried out to evaluate the potential of chitosan alone and in combination with various agricultural wastes for the management of rootknot nematode, Meloidogyne incognita on eggplant cv. ‘BR-112’ under greenhouse conditions. The results showed that chitosan as a single or joint treatment with agricultural wastes significantly (p ≤ 0.05) reduced root-knot indices, and the nematode population in soil. As a result, of this, the growth and growth yielding attributes of eggplant were remarkably augmented. Chitosan as an elicitor induced plant mediated systemic resistance against M. incognita in eggplant. The results of the study demonstrated that maximum reduction in eggmass/root, eggs/eggmasses, nematode population and root-knot indices, was acquired by the treatments: chitosan + onion and chitosan + mentha. It was followed by chitosan + Brassica, chitosan + urad and chitosan + coconut whereas, chitosan combined with corn cob waste was found to be the least effective when compared to the control. The application of chitosan alone was effective but not very satisfactory. Compared to the control applications of all the treatments significantly increased plant growth in terms of length, fresh and dry weights, pollen fertility, yield and biochemical parameters such as chlorophyll, carotenoid content and antioxidant enzymes. This may have been due to the eliciting activity of chitosan, causing systemic resistance in the plant and the release of various toxic chemical compounds during decomposition which have lethal effects against the second stage juveniles of M. incognita and nematode multiplication.
The response of the Mi-1 gene to different densities of Meloidogyne incognita race 2 was investigated under controlled conditions. Susceptible and resistant tomato seedlings were inoculated with 25, 50, 100, 200, 400, 1000, 2000, 5000 and 10 000 second-stage juveniles of M. incognita. Plants were uprooted 8 weeks after inoculation and the numbers of egg masses and galls on the roots, and second-stage juveniles in 100 g soil per pot were counted. In susceptible plants, there was a correlation between the number of egg masses on roots until 2000 J2 inoculum densities. In resistant plants, when inoculum densities increased, the number of egg masses and galls also increased. The reproduction factor ratio was >1 in the susceptible plant and <1 in the resistant plant. The data showed that the 5000 J2 inoculum was a critical limit, and 10 000 J2s were above threshold for resistant plants. The data indicate that densities of M. incognita can seriously affect the performance of the Mi-1 gene.
Soil amendments with powdered seeds of Trigonella foenum - graecum (fenugreek) caused soil suppressiveness against Meloidogyne javanica. Decomposed seeds of fenugreek caused marked reduction in nematode population densities and subsequent root-knot development as compared to the aqueous extract of the seeds indicating that some indirect factors are involved in the suppression of root-knot nematode. Both decomposed seeds and aqueous extracts enhanced plant height and fresh weights of Dat whereas root growth remained uninfluenced. Changes in fungal communities associated with nematode control were studied by comparing population numbers of :ngi in the soil and in internal root tissues (endorhiza) in non-amended and fenugreekanded soils. Acremonium sp., Chaetomium globosum, Fusarium solani, Macropho'na plwseolina and Rhizoctonia solani were found to colonize inner root tissues of ugbean. Acremonium sp., C. globosum and F.solani were isolated in a relatively her frequency from roots growing in the amended soils while M. phaseolina and R.solani colonized greatly in roots growing in non-amended soil. Of the fungi isolated soils, Penicillium brefaldianum caused maximum juvenile mortality of M. javanica whereas F.solani caused greatest inhibition of egg hatch.
Three Polish, four other European and three tropical isolates of the nematophagous fungus Verticillium chlamydosporium were screened for their ability to colonise the surface of barley roots, produce chlamydospores and infect eggs of Meloidogyne incognita in laboratory tests. PCR-fingerprinting of different Polish isolates and Vc10 was used to detect differences between isolates. Among the European isolates, the Polish isolate which came from a field fertilised with manure produced the greatest number of viable chlamydospores, the largest proportion of infected eggs of Meloidogyne incognita and the most prolific root colonisation. Among the tropical isolates, an isolate A produced the largest number of chlamydospores and an isolate B was the most prolific root coloniser and parasitised most of M. incognita eggs.
Efficacy of two strains of Pseudomonas aeruginosa (Pa-5 and IE-2) and aBacillus subtilis isolate alone or in conjunction with neem cake or Datura fastuosa was tested for the management of three soilborne root-infecting fungi including Macrophomina phaseolina, Fusarium solani and Rhizoctonia solani and the root-knot nematode, Meloidogyne javanica on uridbean. Biocontrol bacteria used in combination with either neem cake or D. fastuosa gave better control of the root-rot and root-knot infection with the enhancement of growth of uridbean compared to the use of either component alone. Neem cake 1 % w/w mixed with P. aeruginosa strain lE-2 caused greatest inhibition of the root-knot development due to M.javanica. P. aeruginosa and B. subtilis used with organic amendment also increased Bradyrhizobium-nodules in the root system.
The potential of 3 Pseudomonas aeruginosa strains as biocontrol agents of rootinfecting fungi Macrophomina phaseolina, Flusarium solani and Rhizoctonia solani and the root-knot nematode Meloidogyne javanica Was tested on chi!i and uridbean under greenhouse conditions. All the three strains significantly reduced nematode populations in soil, invasion, multiplication and gall formation due to M. javanica. Root infection by fungi was also effectively suppressed following P. aerllginosa application. Bacterial antagonists exhibited better biocontrol and growth promoting activity in 15-day-old plants than did those harvested at 30 or 45 days. Population of the bacterium in the rhizosphere declined rapidly after 15 days of nematode inoculation. Strain Pa-5 showed maximum nodulation in 15-day-old samplings while strain Pa-7 showed highest number of nodules in 30 and 45-day-old uridbean plants.
Leaves, stem and pneumatophore of Avicennia marina and leaves and stem of Rhizophora mucronata were used as the organic amendments at 0.1, 1 and 5% concentrations in the control of root rot fungi like (Fusarium spp., Rhizoctonia solani and Macrophomina phasoelina) and root knot nematode Meloidogyne javanica on potato. In pot experiments, germination of seeds, shoot length, shoot weight, root length, root weight and number of knots were signifi cantly increased when plant parts like leaves, stem and pneumatophore of A. marina and R. mucronata were used at 1 and 5% concentrations. There was a complete suppression in infection of R. solani and M. phaseolina when A. marina and R. mucronata were used at 5% concentration on potato. Maximum inhibition of knots of M. javanica was observed when powder made from mangrove plant parts was used at 1 and 5% concentrations. Powder from all plant parts, like leaves, stem and pneumatophore, was effective in suppression of root infecting fungi and root knot nematode.
Root-knot nematodes have become a grave menace to the lucrative production of cucumber throughout the world. These nematodes are mainly controlled by applying nematicides, but their use is often associated with hazards. Alternatively, the use of nematode resistant cultivars is considered to be innocuous and economically feasible. For their fitness as nematode-suppressive crops, the reduction in growth and yield parameters of these cultivars must be assessed. As there is little documented data about the effects of Meloidogyne incognita on the damage of cucumber, therefore, in the present study, the effects of M. incognita were evaluated on growth and yield parameters of fifteen cucumber cultivars. M. incognita significantly negatively affected the growth and yield parameters of all the cucumber cultivars. Shoot and root lengths and shoot weights of all the cultivars were significantly reduced as a result of nematode infection. Maximum reductions in these parameters were recorded in highly susceptible cultivars followed by susceptible ones, while the reductions were minimal in resistant followed by moderately resistant cultivars. On the contrary, the infection by M. incognita resulted in an increase in root weights of all the cultivars. The increase was found to be the maximum in highly susceptible cultivars followed by susceptible and moderately susceptible cultivars. Likewise, the minimum increase was observed in the resistant cultivars followed by moderately resistant cultivars. Similarly, significant variations in yield parameters among fifteen cucumber cultivars were also recorded as a result of M. incognita infection. In the case of highly susceptible cultivars, the reductions in yield parameters were maximum, whereas the reductions in resistant and moderately resistant cultivars were found to be minimum. As cultivars Long Green, Marketmore, Pioneer-II, Dynasty and Summer Green experienced no significant damage compared to susceptible cucumber cultivars and therefore, they are approved for cultivation in nematode infested soils.
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