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The assessment of black cherry seedlings root system was carried out at the Didactic-Experimental Establishment of the UWM in Olsztyn in September 2002. The seeds from which the examined seedlings were obtained had been sown into the ground on 2 October 2001. The following seeds were used for the experiment: fresh, sown into the ground immediately after obtaining them from fruit, partly dried seeds and seed sown a month later than in the preceding case as well as physiologically immature seeds and whole fresh fruit. The root systems of seedlings obtained from seeds stored for 1 year and 2 years in open and closed “Twist” type jars at room temperature and in a freezer were also assessed. The largest proportions of seedlings were obtained from seeds stored for 1 year and 2 years in a freezer. The lowest proportions of seedlings were obtained from seeds sown a month later and from whole fresh fruit. In this experiment the most favorable development of the root system was observed in case of seedlings from seeds stored for 2 years in an open jar. The time from picking to sowing of seeds was of little importance. The seedlings from partly dried seeds and later sowing were characterized by the lowest mass of the root system.
The distribution of lead in root tips of Dianthus carthusianorum was compared in populations from a zinc-lead waste heap in Bolesław near Olkusz and from a natural stand in the Botanical Garden in Lublin. The analyses were made at two developmental stages: seedlings (after 8 days of incubation in 5 mg/dm3 Pb+2 from PbCl2 in 1/8 Knop medium) and mature plants (after 23 days of incubation in 10 mg/dm3 Pb+2 from PbCl2 in 1/2 Knop medium). Histochemical methods (rhodizonate and dithizonate) of lead detection revealed significant accumulation of this metal on the root surface of the examined plants. The site of next-strongest lead accumulation in root tips of plants from both populations was in cell walls of the pericycle. The layer of meristematic pericycle cells seemed to be a strong barrier against penetration of lead to the deepest cells of the procambium. Histochemical methods and tissue sections revealed no differences in lead distribution between root tips from the waste heap and from the natural population, but differences were detected on the ultrastructural level. There were numerous lead deposits in the cytoplasm of cells from ground meristem in the natural population, and none in specimens from the waste heap, indicating that lead had a higher toxic effect on the natural population of D. carthusianorum.
In the years 1998–2000 an investigation was carried out on ‘Jonagold’ apples harvested from 6-year old trees grafted on M.9, M.26, P 2, P 22 and P 60. In 1998 and 1999 the average calcium content in fruit was significantly higher than in 2000. The highest calcium was observed in the peel of apples, in each of the year. The effect of the rootstock on the level of calcium in the calyx, core and pedicle part of apples changed, depending on the vegetation season. In each year of the experiment the strongly dwarfing rootstock P 22 had the smallest share in the accumulation of calcium in the different parts of apples.
Sour cherry (Prunus cerasus L.) is one of the most important fruit crops in Poland. There are many varieties cultivated in orchards, but only a few of them play an important role in commercial production. These few varieties have been the object of numerous studies focused on practical aspects like growth performance, yielding, or resistance to diseases. Recently more belowground research has been carried out in pomological plants using the minirhizotron research allowing to observe roots in short and long term experiments. There have been very few studies concerning root growth dynamics of sour cherry cultivars. Here we studied the influence of four major factors on root growth: the cultivar, root diameter, soil depth, and season on the survivorship of fine roots. We used the minirhizotron technique (MR) to examine fine roots dynamics of four sour cherry cultivars grafted on the Mahaleb rootstock, grown in an experimental orchard in Central Poland. The results revealed that the greatest impact on root survivorship was exerted by root diameter, depth of root formation and the season, whereas cultivars had no obvious influence. The finest roots (with a diameter <0.2 mm) and roots formed at a depth of down to 10 cm below the soil surface had the shortest survivorship. On the other hand, thicker roots (>0.75mm) and roots formed at a depth of more than 50 cm below the soil surface have the longest survivorship. The season of root growth has little impact on root survivorship, but has a big influence on the number of the roots formed. There is no impact of the cultivar on the differences in observed roots survivorship.
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Root system quality is very important for maize growth, development and productivity. The maize breeding, generally, is focused on the performance of aboveground part of plant. It is interesting to compare the relationship between root and shoot of maize studied in divergent conditions. Results of the study of the effects of climatic regimes on variations among maize genotypes were described in paper. The genotype x environment interaction (GEI) are analysed through factorial regression models in order to explicate of the effects of environmental factors on root growth. Chosen statistical method is very helpful tool to evaluate the environmental effect on genetic variability of maize root traits. There was confirmed significant effect of GEI on the root traits. GxE interaction has simply interpretation, because it can be described by the use of linear relations among covariates. It concerns soil and air temperature and water availability (EVTP or rainfall amount).
W latach 2000-2002 w Kruszynie Krajeńskim w pobliżu Bydgoszczy prowadzono badania, których celem było określenie, jakim zmianom ulegną cechy morfologiczne (masa i długość) systemu korzeniowego owsa (Avena sativa L.) odmiany Dragon oraz plon ziarna w wyniku zaprzestania stosowania wieloletniego deszczowania i nawożenia azotem na glebie bardzo lekkiej. Stwierdzono, że wpływ następczy deszczowania spowodował zmniejszenie się masy i długości korzeni oraz obniżenie plonu ziarna w pierwszych dwóch latach od chwili zaniechania tego zabiegu. Dopiero w trzecim sezonie wegetacyjnym nastąpiło wyrównanie wielkości plonu między obiektami doświadczenia. Nie stwierdzono natomiast negatywnego wpływu następczego nawożenia azotem na analizowane cechy uprawianego owsa. Wcześniejsze deszczowanie obiektów oraz brak nawożenia sprzyjały rozgałęzianiu się korzeni w profilu glebowym. Jednocześnie stwierdzono, że brak deszczowania rozszerza stosunek między plonem ziarna a masą korzeni oraz między plonem ziarna a stopniem rozgałęzienia korzeni. Mniejszy stopień rozgałęzienia korzeni sprzyja wytwarzaniu relatywnie wyższego plonu ziarna.
Some results of first trials within the frames of project "Principles of selection of maize genotypes with low nitrogen fertiliser requirements" are presented in the paper. Experiments were conducted on the rinsed sand in Mits- cherlich's pots and in the fields. Fifty-eight inbred lines of maize from breeding company „Hodowla Roślin Smolice" Ltd. were evaluated at two N level in the substrate: without N and 2.25 g N.pot-1 or with only natural N level in the soil and 150 kg N.ha-1 in field experiment. In studied base of maize inbred lines large genetic variability of the response to reduced N supply in the substrate is described. Yield of evaluated maize lines was not closely related to N Nutrition Index (NNI) in the case of nitrogen deficiency. Some root traits: root volume and root N status seem to be related to maize adaptation to N deficiency in substrate. There are indicated groups of genotypes with similar response on N stress for further, more detailed experiments.
Pot experiments were designed to test the applicability of root electrical capacitance measurement for in situ monitoring of root water uptake activity by growing cucumber and bean cultivars in a growth chamber. Half of the plants were inoculated with Funneliformis mosseae arbuscular mycorrhizal fungi, while the other half served as non-infected controls. Root electrical capacitance and daily transpiration were monitored during the whole plant ontogeny. Phenology-dependent changes of daily transpiration (related to root water uptake) and root electrical capacitance proved to be similar as they showed upward trends from seedling emergence to the beginning of flowering stage, and thereafter decreased continuously during fruit setting. A few days after arbuscular mycorrhizal fungi-colonization, daily transpiration and root electrical capacitance of infected plants became significantly higher than those of non-infected counterparts, and the relative increment of the measured parameters was greater for the more highly mycorrhizal-dependent bean cultivar compared to that of cucumber. Arbuscular mycorrhizal fungi colonization caused 29 and 69% relative increment in shoot dry mass for cucumbers and beans, respectively. Mycorrhization resulted in 37% increase in root dry mass for beans, but no significant difference was observed for cucumbers. Results indicate the potential of root electrical capacitance measurements for monitoring the changes and differences of root water uptake rate.
Tractor traffic as a cause of soil compaction is widely recognized as one of the most important factors responsible for environmental degradation and plant yield losses. It is a serious problem for perennial crops, where the soil surface is wheeled without any opening operation. The aim of our study was to evaluate the effect of tractor traffic on meadow fescue (Festuca pratensis) yields and root development. The field experiment was located in Mydlniki near Kraków, Poland, on silty loam Mollic Fluvisol. Experimental plots were established in randomized block design with four replications. Four compaction treatments were applied using the following range of number of passes: (P0) untreated control, (P2) two passes, (P4) four passes and (P6) six passes completely covering plot surfaces after each harvest. The dry matter (DM) of the yield and roots (RMD) were determined. Morphometric parameters of roots were estimated using image analysis software. Root length density (RLD), specific root length (SRL), and mean diameter (MD) were calculated. Tractor traffic resulted in significant influence on meadow fescue annual yields. The highest annual yields were obtained at the P2 and P4 treatments. However, in the first cut it was noticed that an increase in the number of passes increased plant yields. During the second and the third cut it was found that intensive tractor traffic decreased plant yields, probably as an effect of damage caused to above-ground parts of plants. The meadow fescue roots were significantly affected by tractor traffic only in the 5-15 cm soil layer. Tractor traffic increased the RLD value in a root diameter range of 0.1-0.5 mm. However, any other morphometric parameters, like mean root diameter (MD), specific root length (SRL) or dry root diameter (RDM) were not affected by soil compaction.
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