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The aim of the study was to determine the effect of various biopreparations on the growth of the apple root system, the number of spores of AMF, the total number of bacteria and microscopic fungi in the rhizosphere soil, and the degree of mycorrhizal association in the roots of two apple tree cultivars. The experiment was established in the spring of 2009 in the Experimental Orchard of the Institute of Horticulture in Dąbrowice. The research objects were one-year-old maidens of the apple cultivars ‘Topaz’ and ‘Ariva’ grafted on M26 rootstock. The trees were treated with the following biopreparations: control, control NPK (standard NPK fertilization), manure, Micosat F + manure, Humus UP, Humus Active + Aktywit PM, BioFeed Amin + manure, BioFeed Quality + manure, Tytanit + manure, Vinassa + manure, Florovit Eko, and Florovit Pro Natura. Treatment of ‘Topaz’ and ‘Ariva’ apple trees with the biopreparations Micosat F, Humus UP, Humus Active + Aktywit PM, BioFeed Amin, Vinassa, Florovit Eko and Florovit Pro Natura produced positive effects on the growth of apple roots and their mycorrhizal frequency, and the size of the populations of microorganisms in the rhizosphere soil.
The effects of different concentrations of Al (10 μM, 50 μM, 100 μM) on nucleoli in root tip cells, root growth, antioxidant enzyme activity and malondialdehyde (MDA) content were investigated in hydroponically grown Vicia faba L. Aluminum significantly inhibited root growth of V. faba treated with 50 μM and 100 μM Al. In the nucleolus in root tip cells, some particulates containing argyrophilic proteins were extruded from the nucleus into the cytoplasm, and some were scattered in the nucleus after Al stress. Superoxide dismutase (SOD) activity in leaves and roots exposed to different concentrations of Al was mostly higher than in the control. Seedlings exposed to 100 μM Al showed significantly higher peroxidase (POD) activity in roots than in the control. POD activity increased much more in roots than in leaves. Catalase (CAT) activity was lower in roots than in leaves. Malondialdehyde (MDA) content in leaves and roots of plants exposed to 50 μM and 100 μM Al was significantly higher than in the other groups and the control at 6 to 9 days of treatment. These results suggest that alterations in nucleoli and altered antioxidant enzyme activity and MDA content in V. faba can serve as useful biomarkers for detection of Al toxicity. The mechanisms of Al toxicity and tolerance in V. faba are briefly discussed
An experiment was carried out in a greenhouse of the Research Institute of Horticulture (RIH) to evaluate the effect of new organic fertilizers and amendments on root growth and mycorrhizal abundance and species richness in the rhizosphere of strawberry plants cv. 'Elsanta'. The plants were grown in rhizoboxes (sized 37 cm x 1.8 cm x 20 cm), filled with 1.85 kg of a podsolic soil collected from an uncultivated field of an experimental organic orchard of the RIH. The soil characteristics were: pH 5.5, organic matter content 1.5%, P content 51 mg P kg-1, K content 158 mg K kg- 1 . The plants were treated with different organic fertilizers and amendments: dry granulated bovine manure (Doktor O'grodnik), extract of vermicompost (Humus UP), extract of humates (Humus Active + Aktywit PM), plant extract (BioFeed Amin), extract from several seaweed species reinforced with humic and fulvic acids (BioFeed Quality), a consortium of beneficial soil organisms (Micosat), a stillage from yeast production (Vinassa) and a solution of titanium (Tytanit). Plants treated with BioFeed Amin, BioFeed Quality, Micosat, Vinassa and Tytanit received also half dose of dry manure. A standard NPK fertilization (NPK control) and a not fertilized control were also included. The following parameters were measured: root growth and morpho­logical parameters, number of arbuscular mycorrhizal fungi (AMF) spores, mycorrhizal frequency of AMF in the roots. The chemical composition of the applied products and of soil were also determined.
The physical characteristics of soil aggregates influence soil tilth, surface sealing, water infiltration and root growth. Soil management and compaction significantly affect these characteristics. The aim of this work was to determine the effects of tractor traffic across the slope on bulk density, water stability, tensile strength and sorptivity of aggregates from grass covered and cultivated sloping (18%) vineyard soils. The grass covered (G) treatment included periodical mowing and cutting back of herbage and cultivation (C) treatment consisting of autumn ploughing (18 cm) and spring and summer rotary hoeing in the vineyard inter-row zones (2.7 m). A crawler tractor (2.82 Mg) was used in the inter-row zones, moving across the slope for all tillage and chemical operations. Soil aggregates were taken from the inter-rut and the upper and lower rut areas (0-10 and 20-30 cm) in the inter-row zones and then air-dried. Bulk density and tensile strength were lowest in the inter-rut areas and highest in the lower crawler rut. Aggregate water stability was greater under the lower rut and sorptivity in the inter-rut area in comparison to the remaining inter-row areas. In comparable inter-row areas, water stability and sorptivity of soil aggregates were greater and lower under G than C, respectively. The differences in bulk density and tensile strength between G and C were not consistent and varied depending on the inter-row zone and depth.
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