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The aim of the study was an in vitro assessment of the antimicrobial efficacy of water, methanol and ethanol extracts of Korean ginseng tea, which is widely used in Asian folk medicine. Nine test microorganisms were used: Escherchia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Proteus mirabilis, Bacillus subtilis, Bacillus cereus, and Salmonella enteritidis. The minimum inhibitory concentration (MIC) of active extracts was determined by using a macro-broth-dilution assay. Methanol and ethanol extracts from the tea were active against some bacterial strains while only the water extract was active against all bacterial strains. The MIC values for active extracts ranged between 7.5 and 500 mg/ml. The results of the study indicate that ginseng may be safely used in the treatment of some infectious diseases.
Both seed and root extracts of the medicinal plant, Ashwagandha, Withania somnifera exhibit insect growth regulatory activity against the polyphagous pest, Pericallia ricini. Topical administration of W. somnifera seed and root extracts to last instar larvae of P. ricini disrupted moulting and metamorphosis, leading to a number of developmental abnormalities such as delay in larval-pupal and pupal-adult ecdysis, formation of larval-pupal, pupal- -adult and larval-pupal-adult mosaics/chimeras, ecdysial failure, suppression of pupation and adult emergence and formation of abnormal pupae and adultoids. The treatment with seed extracts was more severe than that of root extracts as it completely suppressed the pupation and adult emergence. The results clearly suggest that the medicinal plant, W. somnifera acts as a potential insect growth regulatory (IGR) disrupting the moulting and metamorphosis as a consequence of interference with the endocrine system.
The present study was quantitative analysis and antimicrobial activity of petroleum ether, chloroform, ethyl acetate, methanol root extracts. The gram positive and gram negative bacterial and fungal species by the disc diffusion method at various concentration (250μl-1000μl). The results was present in the phenolic and flavonoid content compared to extracts. All the root extract against to Bacillus subtilis and Streptococcus pyogenes and fungal species Candida albicans at highest inhibition at concentration of methanolic root extract of zone formation. The preliminary studies on Decalepis hamiltonii root extract exhibited their antimicrobial potential which could be exploited further and future pharmaceutical, therapeutic efficiency and food preservation and applications.
Background. Experimentally, the root extract of Salacia reticulata has been found to have potent hypoglycemic activity both in normal and in streptozotocin-induced diabetic rats. Furthermore, the decoction of S. reticulata roots is used in the treatment of rheumatism, gonorrhea, itching and swelling, asthma, thirst, amenorrhea and dysmenorrhea. The aim of the study. The efficacy of the plant extract of Salacia reticulata Wight (hippocrataceae) was tested on albino rats (Wistar strain) in which diabetes were induced by administration of alloxan. Methods. The rats were grouped into diabetic control, insulin treated and various extracts of Salacia reticulata Wight treated. The effect of extract on blood glucose level after 15 and 30 days of treatment, serum cholesterol and triglyceride level was assessed after 30 days of treatment. Results. The fall in the blood glucose level in rats treated with ethyl acetate fraction was more followed by ethanol extract, insulin treatment, water extract and diethyl ether extracts. The insulin treated rats had a significant fall in serum cholesterol level followed by ethanol, diethyl ether and petroleum ether extracts. Serum triglyceride level was not influenced by any of the extract treatment. Conclusions. Our study confirms the percentage of hypoglycemic effect exerted by all the different fractions almost correspond with the level of action exerted by insulin. Hence we strongly recommend the use of Salacia reticulata Wight extract in diabetic condition
This work assesses the antibacterial activity of plumbagin (5-hydroxy-2-methylnaphthalene-l,4-dione) and of methanol, chloroform and aqueous extracts of Plumbago zeylanica L. root against various pathogenic bacteria, and the minimum inhibitory concentrations (MICs). Plumbagin and chloroform extracts of Plumbago zeylanica L. root showed antibacterial activity against Escherichia coli, Salmonella typhi and Staphylococcus aureus. Inhibition against Klebsiella pneumoniae, Serratia marcescens and Bacillus subtilis was moderate, and lower against Proteus vulgaris and Pseudomonas aeruginosa. The methanolic extract exhibited moderate activity and the aqueous extract weak activity against the bacterial strains as assessed by disc diffusion assays. The bioactive compound plumbagin and extract of Plumbago zeylanica root show a wide spectrum of antibacterial activity. The compound shows promise as a new drug for various bacterial infectious diseases.
We studied the influence of Withania somnifera (Ws) root extract (100 mg/kg body weight) on parkinsonism induced by 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP; i.p, 20 mg/kg body weight for 4 days), via the analysis of behavioral features and the oxidant-antioxidant imbalance in the midbrain of mice. A significant alteration in behavior, increased levels of thiobarbituric acid reactive substance (TBARS), and increased activities of superoxide dismutase (SOD) and catalase (CAT) were noticed in this region of brain in MPTP-treated mice. Oral treatment with the root extract resulted in a significant improvement in the mice’s behavoiur and antioxidant status, along with a significant reduction in the level of lipid peroxidation. The results indicated that at least part of the chronic stress-induced pathology may be due to oxidative stress, which is mitigated by Ws. Further studies are needed to assess the precise mechanism to support the clinical use of the plant as an antiparkinsonic drug.
The effects of water extracts of Chenopodium album leaves and roots on the growth of grass weed (Setaria viridis) and broad leaf weed (Corchorus olitorius) grown with beans (Phaseolus vulgaris) in greenhouse pots were studied in the National Research Centre, Giza, Egypt. In this experiment fresh leaf and root extracts and their corresponding dry leaf and root extracts at different concentrations were used. There were significant inhibitions in the dry weights of S. viridis and C. olitorius by all extracts at the flowering stage of beans and at harvest. The inhibition effect of all C. album extracts on both weeds (dry weight/pot) depended on the extracted plant organ (leaf or root), its fresh or dry form as well as its concentrations. The inhibition caused by the leaf extract was much higher on weed growth than that of root extract. A higher concentration of fresh leaf extract (25%) had the highest significant inhibition effect. The results also indicated that C. olitorius was more sensitive to the extracts than S. viridis. On the other hand, the inhibition effect of the extracts on the growth of both weeds was accompanied by increased bean growth and yield/plant. The analysis of both leaf and root extracts of C. album revealed that the total content of polyphenols and flavonoids in the leaf extract was more than triple that of the root extract. The results suggested that the fresh leaf extract of C. album may be a possible tool for the development of weed control using natural herbicides.
Natural supplementations are used in agriculture nowadays not only for improving plant performance but also for reducing the contamination of plant edible parts. Two field trials were conducted to study the potential effects of licorice root extract (LRE; 0.5%) on performance, physio-biochemical components, antioxidant defense system, and contaminants concentrations of Capsicum annuum L. plants grown on a saline soil contaminated with heavy metals. LRE was applied in single (i.e., as rhizosphere application with drip irrigation water; -RA or as foliar spray; -FA) or in integration (i.e., LRE-RA + LRE-FA) treatment. The results showed that both single or integrative treatments significantly increased plant growth and yield, leaf concentrations of photosynthetic pigments, free proline, total soluble sugars, N, P, and K⁺, ratio of K⁺/Na⁺, and activities of CAT, POX, APX, SOD and GR, while significantly reduced contaminants; Na⁺, Cd, Cu, Pb and Ni concentrations in plant leaves and fruits on heavy metals-contaminated saline soil compared to the control (without LRE). Additionally, the integrative LRE-RA + LRE-FA treatment significantly exceeded both single treatments in this concern, which had been recommended for maximizing pepper plant performances with minimizing heavy metals in fruits on contaminated saline soils.
Alcoholic liver disease (ALD) is one of the most common diseases in modern society. A large number of studies are in progress aiming to identify natural substances that would be effective in reducing the severity of ALD. Although there are currently a number of drugs on the market, their long-term use can have numerous side effects. Hemidesmus indicus is an indigenous Ayurvedic medicinal plant used in soft drinks in India. In this study, we examined the effects of its ethanolic root extract on experimental liver damage in order to evaluate its hepatoprotective effects against hepatotoxicity induced in rats by ethanol at a dosage of 5 g/kg body weight for 60 days. The H. indicus root extract was given at a dose of 500 mg/kg body weight for the last 30 days of the experiment. The animals were monitored for food intake and weight gain. The liver was analysed for the degree of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) and antioxidant status using the activities of glutathione-depedendant enzymes. The degree of liver damage was analysed using serum marker enzyme activities, the total protein, albumin, globulin, ceruloplasmin and liver glycogen contents, and the A/G ratio. The Fourier transform infrared spectra (FT-IR) of the liver tissues were recorded in the region of 4000–400 cm−1. The ethanol-fed rats showed significantly elevated liver marker enzyme activities, lipid peroxidation levels and reduced antioxidant levels as compared to the control rats. Oral administration of H. indicus for the latter 30 days resulted in an increased food intake and weight gain, decreased TBARS levels, near normal levels of glutathione-dependent enzymes, increased total protein, albumin, globulin and liver glycogen contents, an increased A/G ratio, and decreased liver marker enzyme activities and ceruloplasmin levels. The relative intensity of the liver FT-IR bands for the experimental groups were found to be altered significantly (p < 0.05) compared to the control samples. For the group that had H. indicus co-administered with ethanol, the intensity of the bands was near normal. Moreover, the results of the FT-IR study correlated with our biochemical results.
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