Wyniki wyszukiwania

1

Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiflorum A.DC.

100%

Urbanska N., Nartowska J., Skorupska A., Ruszkowski D., Giebultowicz J., Olszowska O.

Herba Polonica

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2009

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tom 55

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nr 3

103-108

The sum of saponins in the hairy root lines (6, 17) of Platycodon grandiforum A.DC. was compared. Hairy root line 6 showed a higher total saponin content (6.92%) than the line 17 (6.01%). According to the Chinese Pharmacopoeia standards the content of saponins in Platycodi Radix should be not less than 2%. Our results seem to indicate that the hairy root culture of Platycodon grandiforum A.DC. is a good source of saponins. The Haemolytic Index of the hairy root line 6 was 1600. Digitonin was used as a reference. Moreover, the haemolytic activity of TLC subfractions of saponins varied.

2

Preliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro

100%

Pietrosiuk A., Furmanowa M.

Acta Societatis Botanicorum Poloniae

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2001

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tom 70

|

nr 4

3

Elicitation of thiophene production by cultured hairy roots of Tagetes patula

100%

Rajasekaran T., Madhusudan R., Ravishankar G. A.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 3

4

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The influence of selenium on antifungal activity of secondary metabolites of transformed root cultures of Tropaeolum majus, Schkuhria pinnata and Saussurea nepalensis

84%

Bogusz D., Janas J., Wielanek M., Sklodowska M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 3

5

Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor

84%

Grzegorczyk I., Wysokinska H.

Acta Societatis Botanicorum Poloniae

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2010

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tom 79

|

nr 1

7-10

The study focused on the production of compounds with antioxidant activity in hairy root and shoot cultures of Salvia officinalis grown in laboratory-scale sprinkle nutrient bioreactors. HPLC analysis showed that production of rosmarinic acid in transformed roots (34.65±1.07 mg l-1) was higher that in shoot culture (26.24±0.48 mg l-1). In the latter diterpenoids: carnosic acid (1.74±0.02 mg l-1) and carnosol (1.34±0.01 mg l-1) were also found. Biomass accumulation after a growth period in the bioreactor was also studied. An 18-fold increase in hairy root biomass was recorded after 40 days of culture. In sage shoot culture, biomass increased 43 times after 21 days of bioreactor run. The current operating conditions of the bioreactor were not suitable for the propagation of Salvia officinalis mainly due to the hyperhydricity problem of leaves and stems.

6

In vitro root cultures of Lychnis flos-cuculi L. as a potential source of triterpenoid saponins and ecdysteroids

84%

Malinski M., Sienicka J., Thiem B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

7

Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory [Cichorium intybus L. cv. Lucknow local]

84%

Bais H. P., Sudha G., Ravishankar G. A.

Acta Physiologiae Plantarum

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2001

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tom 23

|

nr 3

8

Wild type root cultures of Scutellaria barbata

67%

Wilczanska-Barska A., Krauze-Baranowska M., Majdan M., Glod D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 4

9

A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures

67%

Dechaux C., Boitel-Conti M.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

|

nr 1

Hairy root culture plays an important role in investigation of alkaloid production in culture in vitro. The complexity of scientific work and the production capacity limitations associated with whole plant systems and unorganized cells can be overcome using culture of hairy roots. This paper presents an example of a strategy to produce secondary metabolites from plants, in which we investigated the alkaloid pathway, modified gene expression, and cultivated and optimized hairy root growth in a bioreactor. Datura innoxia whole plant was transformed by Agrobacterium rhizogenes, and the obtained hairy roots were studied for their tropane alkaloid production. Optimization of medium led to an increase of scopolamine production in hairy root culture of Datura innoxia by increasing the produced biomass, but genetic engineering seems to be the best way to increase the accumulation of scopolamine. These root cultures were not able to stably overaccumulate scopolamine. The involvement of putative enzymatic regulation of the tropane alkaloid pathway is discussed.

Ograniczanie wyników

Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiflorum A.DC.

Preliminary results of indole alkaloids production in different roots of Catharanthus roseus cultured in vitro

Elicitation of thiophene production by cultured hairy roots of Tagetes patula

The influence of selenium on antifungal activity of secondary metabolites of transformed root cultures of Tropaeolum majus, Schkuhria pinnata and Saussurea nepalensis

Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor

In vitro root cultures of Lychnis flos-cuculi L. as a potential source of triterpenoid saponins and ecdysteroids

Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory [Cichorium intybus L. cv. Lucknow local]

Wild type root cultures of Scutellaria barbata

A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures