Wyniki wyszukiwania

1

Primary structure of porcine spleen ribonuclease: sequence homology

100%

Kusano A., Iwama M., Sanda A., Suwa K., Nakaizumi E., Nakatani Y., Ohkawa H., Ohgi K., Irie M.

Acta Biochimica Polonica

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1997

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tom 44

|

nr 4

The primary structure of porcine spleen RNase (RNase Psp1) was investigated as a mean of assessing the structure-function relationship of base non-specific ribonucleases of animal origin. N-terminal analysis of RNase Psp1 yielded three N-terminal sequences. These peptides were separated by gel-filtration on Superdex 75HR, after reduction and S-carboxymethylation of RNase Psp1. Determination of the amino-acid sequence of these peptides indicated that the RNase Psp1 preparation consisted of three peptides having 20 (RCM RNase Psp1 pep1), 15 (RCM RNase Psp1 pep2), and 164 (RCM RNase Psp1 pro) amino-acid residues, respectively. It possessed two unique segments containing most of the active site amino-acid residues of the RNases of the RNase T2 family. The alignment of these three peptides in RNase Psp1 was determined by comparison with the other enzymes in the RNase T2 family. The overall results showed that RCM RNase Psp1 pep1 and RCM RNase Psp1 pep2 are derived from the N-terminal and C-terminal regions of RNase Psp1, respectively, probably by processing by some protease. The molecular mass of the protein moiety of RNase Psp1 was 23235 Da.

2

The response of the double strand-specific nuclease V1 to Y-base removal in yeast tRNA(Phe)

88%

Marciniec T., Ciesiolka J., Krzyzosiak W.

Acta Biochimica Polonica

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1989

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tom 36

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nr 2

3

Endoribonuclease from roots of the Vicia faba L. ssp.minor seedlings

75%

Kazmierczak A., Knypl J. S.

Acta Biochimica Polonica

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1993

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tom 40

|

nr 1

4

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Human ribonuclease Dicer inhibition using RNA aptamers

63%

Tyczewska A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2014

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tom 95

|

nr 4

5

Purification and characterization of nuclease I associated with rye germ ribosomes

63%

Siwecka M. A., Rytel M., Szarkowski J. W.

Acta Biochimica Polonica

|

1989

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tom 36

|

nr 1

6

Poly-C specific ribonuclease activity correlates with increased concentrations of IL-6, IL-8 and STNFR55-TNFR75 in plasma of patients with acute pancreatitis

63%

Naskalski J. W., Kusnierz-Cabala B., Panek J., Kedra B.

Journal of Physiology and Pharmacology

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2003

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tom 54

|

nr 3

Plasma pancreatic-type Poly-C specific ribonuclease (P-RNase) - enzyme activity increases in patients with acute pancreatitis (AP) who develop pancreatic necrosis and severe disease course. It is considered as a marker of pancreatic tissue destruction. The aim of this study was to estimate interrelations between major inflammatory cytokines such as: interleukin 6 (IL-6), interleukin 8 (IL-8) and tumor necrosis factor soluble receptors: sTNFR55 and sTNFR75 output, and plasma P-RNase activity. The study was carried out in a group of 56 patients with AP, where 20 developed pancreatic necrosis. It was found that serum P-RNase concentration and levels of all studied inflammatory cytokines significantly increase already in the first day from diagnose of the disease (2,5 folds for P-RNase, 20 for IL-8, about 200 for IL-6 and 1,5 for receptors, respectively). In the first day from admission to hospital, P-RNase activity significantly correlated with plasma concentration of studied inflammatory cytokines. The most pronounced correlation was found for P-RNase and IL-6 in days 1-4 from diagnose, manifested by Pearson correlation r coefficients amounting to 0,86; 0,79; 0,60 and 0,57 respectively (p<0,001). Dividing the studied AP patients into two groups, varying in severity of disease a significant differences in P-RNase and IL-6, IL-8 and sTNFR55/sTNFR75 were found. In patients with acute necrotizing pancreatitis P-RNase significantly correlate with levels of major inflammatory cytokines. Carried out studies suggest that activity of P-RNase reflects severity of inflammatory reaction, which is dependent on development of pancreatic injury and tissue necrosis in AP.

7

Sensitivity of Spirodela oligorrhiza to paclobutrazol and other plant growth retardants

63%

Knypl J. S., Romanowska-Duda Z.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 2

8

Assessment of a method of determining ribonuclease activity employing RNA as substrate

63%

Naskalski J. W.

Folia Biologica

|

1993

|

tom 41

|

nr 3-4

9

The ribonuclease activity of the two synthetic polypeptides having zinc finger sequence

63%

Giel M., Rekowski P., Kupryszewski G., Barciszewski J.

Acta Biochimica Polonica

|

1993

|

tom 40

|

nr 1

10

Exogenous abscisic acid increases stability of polysomes in embryos of triticale caryopses during germination

63%

Weidner S., Kazarnowicz M., Fraczek E., Amarowicz R., Karamac M.

Acta Physiologiae Plantarum

|

2006

|

tom 28

|

nr 6

Some posttranslational processes that occur in embryos of germinating triticale caryopses treated with different concentrations of abscisic acid (ABA) were examined. ABA increased the ratio of cytoskeleton-bound polysomes in the total population of polysomes and depressed the share of free and membrane-bound polysomes. Using exogenous RNase, stability of the total polysomal population as well as each polysomal fraction was investigated. The total extractable polysomes isolated from embryonic tissues of germinating triticale caryopses treated with ABA were more stable than the polysomes isolated from the control sample caryopses. The contribution of the polysomes that were not digested by RNase was increased by higher concentrations of ABA applied during germination. At high concentrations of ABA (50, 100 pM), the quantitative contribution of polysomes in the total ribosomal fraction was almost 100 % ofthe amount ofpolysomes before digestion and the modifications observed consisted mainly of the shift of the socalled heavy polysomes towards light polysomes, containing a few ribosomes. Within each polysomal population, cytoskeleton-bound polysomes (CBP and CMBP) were the most stable, which may imply that the bonds between polysomes and these protein filaments, created in all eukaryotic cells increased their stability. It is assumed that mRNAs are stabilised or destabilised by interaction of proteins with their various sequences. A plant hormone may depress or elevate the quantities of these proteins, thus regulating the stability of different mRNAs. The results confirm the multi-faceted mechanism of ABA-induced response, where one of the constituents is the effect ofABA on the stability ofmRNAs molecules. The coordinated regulation ofmRNAs synthesis and their stability provide plants with improved adaptability.

11

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

51%

Brosowska-Arendt W., Weidner S.

Acta Societatis Botanicorum Poloniae

|

2012

|

tom 81

|

nr 3

Plant growth throughout the world is often limited by unfavourable environmental conditions. This paper reports results of a study on long- and short-term osmotic stress (−0.5 MPa) followed by a recovery on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating pea (Pisum sativum L.) seeds. Here we show that, under osmotic stress, cytoskeleton-bound polysomes were charaterized by the highest translation activity, which may be indicative of an important role that this population of polysomes plays in the synthesis of the so-called “stress proteins”. We also find out that in response to osmotic stress, new proteins (22.01, 96.47 and 105.3 kDa), absent in the unstressed sample, associated with the total pool of polysomes, whereas the protein of 22.95 kDa, which was present in the embryonic tissue of seeds germinating under unstressed conditions, disappeared. These changes may have affected both the stability and the translational capacity of polysomes.

12

Computer modelling of polymer decomposition by active interactions with enzyme molecules. A simulation of RNA digestion by ribonuclease

51%

Naskalski J. W., Drozdz R.

Folia Biologica

|

1994

|

tom 42

|

nr 3-4

13

Proline accumulation and ribonuclease activity in three barley genotypes during water stress

51%

Gniazdowska-Skoczek H., Bandurska H.

Acta Physiologiae Plantarum

|

1994

|

tom 16

|

nr 4

14

Molecular aspects of plant responses to pathogene

51%

Kuc J.

Biological Bulletin of Poznań. Supplement

|

1997

|

tom 34

15

Identification and properties of the ribonucleases from triticosecale embryos of dormant, non-dormant and ABA-treated caryopses

51%

Weidner S., Fraczek E.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

16

Protein C-mannosylation: facts and questions

51%

Furmanek A., Hofsteenge J.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 3

Among the posttranslational modifications of proteins, glycosylation is probably the most abundant one. Two main types of protein glycosylation have been known for several years, namely N-glycosylation and O-glycosylation. Their biochemical properties, structure and biosynthesis, have been described extensively. Their biological functions are also known for a number of proteins, although in many cases the function remains speculative despite continuous efforts. A few years ago, a new type of protein glycosylation was found, which is different from the above-mentioned ones. It was called C-glycosylation, since the sugar is linked to the protein through a carbon-carbon bond. This article reviews the biochemistry of C-glycosylation, the biosynthetic pathway and structural requirements. Possible biological functions of this modification are also discussed.

17

Wlasciwosci pepsyny jako bialka polianionowego. Cz.III. Degradacja przez pepsyne kompleksujacych z nia bialek zasadowych

38%

Roszkowska-Jakimiec W., Gacko M., Worowska A., Greczaniuk A.

Bromatologia i Chemia Toksykologiczna

|

1998

|

tom 31

|

nr 1

71-74

Określono zdolność pepsyny do degradacji kompleksującej z nią protaminy, histonu całkowitego, histonu H1, H2A, H2B, H3 i H4, cytochromu c, lizozymu i rybonukleazy. Wykazano, że tworzenie kompleksów pepsyny z białkami zasadowymi nie znosi jej aktywności proteolitycznej.

Ograniczanie wyników

Primary structure of porcine spleen ribonuclease: sequence homology

The response of the double strand-specific nuclease V1 to Y-base removal in yeast tRNA(Phe)

Endoribonuclease from roots of the Vicia faba L. ssp.minor seedlings

Human ribonuclease Dicer inhibition using RNA aptamers

Purification and characterization of nuclease I associated with rye germ ribosomes

Poly-C specific ribonuclease activity correlates with increased concentrations of IL-6, IL-8 and STNFR55-TNFR75 in plasma of patients with acute pancreatitis

Sensitivity of Spirodela oligorrhiza to paclobutrazol and other plant growth retardants

Assessment of a method of determining ribonuclease activity employing RNA as substrate

The ribonuclease activity of the two synthetic polypeptides having zinc finger sequence

Exogenous abscisic acid increases stability of polysomes in embryos of triticale caryopses during germination

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

Computer modelling of polymer decomposition by active interactions with enzyme molecules. A simulation of RNA digestion by ribonuclease

Proline accumulation and ribonuclease activity in three barley genotypes during water stress

Molecular aspects of plant responses to pathogene

Identification and properties of the ribonucleases from triticosecale embryos of dormant, non-dormant and ABA-treated caryopses

Protein C-mannosylation: facts and questions

Wlasciwosci pepsyny jako bialka polianionowego. Cz.III. Degradacja przez pepsyne kompleksujacych z nia bialek zasadowych