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The experiment was conducted in the years 1999–2001. The object of the studies was the rhizosphere soil of soybean ‘Polan’ cv., and 0.1% chitosan. This compound was used once (seed dressing), twice (seed dressing and seedling spraying), three times (seed dressing, seedling spraying and plant spraying at anthesis). The experiment also considered a control combination, i.e. without chitosan. The purpose of the studies was to determine the effect of chitosan on the qualitative and quantitative composition of microorganisms in rhizosphere soil with antagonists distinguished. The microbiological analysis of samples of soybean rhizosphere soil taken from particular experimental combinations pointed to different numbers of bacteria and fungi. The greatest total number of bacteria colonies and the smallest number of fungi colonies were characteristic of the rhizosphere soil of soybean after using chitosan three times. This sample of soil contained the greatest number of bacteria and fungi with antagonistic effect towards plant pathogens.
The object of the studies conducted in the years 2000–2002 on a field of 3 years’ monoculture of soybean was rhizosphere soil of soybean cultivated after tansy phacelia, winter wheat, white mustard, rye, agrimony and soybean as previous crops. The purpose of the studies was to determine the effect of cultivating the above listed previous crop plants on the formation of microorganism communities in the rhizosphere soil of soybean. The lowest total number of fungal colonies was found in the rhizosphere soil of soybean cultivated after rye and winter wheat (21.09 × 103 and 22.58 × 103 c. f. u., respectively), while the highest number was found in soil after soybean (36.95 × 103 c. f. u.). The highest total number of bacteria was found in 1 g of dryweight of the rhizosphere soil of soybean cultivated after agrimony, and the lowest after soybean (5.80 × 106 and 4.09 × 106 c. f. u., respectively). The largest proportion of pathogenic fungi was characteristic of the rhizosphere soil of soybean cultivated after soybean, and the smallest – of the rhizosphere soil of soybean after agrimony as a previous crop. The dominating species among pathogenic fungi in all experimental objects was Fusarium oxysporum. The rhizosphere soil of soybean cultivated after soybean was the poorest in saprophytic fungi (35.2% of all isolations). On the other hand, the highest number of saprophytes, including antagonistic ones, was found in the rhizosphere soil of soybean after agrimony and winter wheat.
On plots under barley culture from seeds treated and untreated with the fungicidal preparation Oxafun T, the total numbers of oligotrophic and copiotrophic bvacteria, as well as the numbers methylotrophic and Pseudomonas bacteria, were determined in rhizosphere and in non-rhizosphere soil. It has been found that in non-rhizosphere soil the application of the seed dressing did not significantly change the mean number of the studied bacteria for the period of barley culture. It, however, contributed to proliferation of these microorganisms in rhizosphere zone. Rhizosphere effect of barley was increased to the degree dependent on the group of determined bacteria.
The purpose of the studies was to determine the effect of such preparations as Biochikol 020 PC, Polyversum and Biosept 33 SL on the formation of microorganisms communities in the rhizosphere soil of soybean. Results of the studies pointed to a positive effect of biopreparations on the increase number of cfu total bacteria, Bacillus spp. and Pseudomonas spp. After the application of biopreparations balance between pathogens and saprobionts was maintained in the rhizosphere soil of soybean. Such relation did not exist after using chemical preparations. Biopreparations increased the number of antagonistic bacteria – Bacillus spp., Pseudomonas spp. and fungi – Gliocladium spp., Trichoderma spp.
The study comprised tests on the effect of fungicides: Oxafun T, Funaben T and Baytan Universal on the nitrogenase activity of methylotrophic bacteria, selected from the rhizosphere and non-rhizosphere soil of spring barley. The obtained results indicated that the field dose of fungicides - 0.5 ppm had no effect on all the examined strains of methylotrophic bacteria. The dose of 50 ppm, however, had varied effects, suppressing or stimulating, depending on the particular strain. The highest concentration of fungicides applied, i.e. 100 ppm in all cases suppressed the nitrogenase activity of the strains under examination.
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