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The paper presents the research results of the productivity of miscanthus biomass over a three year period, evaluating the dependency between the quality of the rhizomes and growing conditions in western forest-steppe region of Ukraine. The optimal planting density and mass of rhizomes were studied and classified. Factors affecting the yield of dry matter miscanthus were also analyzed.
In plants, biomass allocation and rhizome morphological plasticity reflect different strategies of adaptation to the environment. The aims of this study were to explore the patterns of allocation to different components of the belowground system, and to evaluate the effects of environmental factors on the plasticity of biomass allocation and rhizome morphology. We conducted a survey of the clonal plant, Scirpus planiculmis, in the Momoge wetland in northeastern China. Samples were collected at three sites. Site I is the washland of a seasonal lake. Site II is a permanent lake. Site III belongs to the bank of a recessive river. The average number of sampled plants was 686.25±91.61 per site. We determined the biomass of different plant parts (aboveground part, root, tuber, rhizome) and measured parameters of rhizome morphology (specific rhizome length, average rhizome diameter). Edaphic factors (soil moisture content, organic matter, available phosphorus, available potassium, pH, electrical conductivity) were included as environmental variables. Differences in biomass allocation patterns were analyzed and multiple regression analyses were used to construct a model. The results showed that the structure of the belowground system varied significantly among the three sites. (1) The tubers accounted for the largest share of biomass in plants at site I; (2) plants at site III showed significantly longer rhizomes than those of plants at site II, while the plants at site II showed larger rhizome diameter; (3) pH was the crucial factor affecting biomass allocation and rhizome morphology, and was negatively correlated with root biomass of S. planiculmis. Based on these results, we concluded that: (1) greater resource allocation to the storage organ (tuber) reflects a conservative strategy to avoid damage and to maintain the potential for recolonization in a frequently disturbed habitat; (2) the morphological plasticity of the rhizome increases the ability of the clone to acquire resources. Shorter rhizomes enable rapid colonization of the habitat and efficient resource use under strong competition pressure, while longer rhizomes are useful to explore new habitats when local resources are insufficient. Our results suggest that ecological niche of S. planiculmis should be reconsidered. It may be more appropriate to classify S. planiculmis as a facultative salt-alkali plant, although a degree of tolerance to salinity and alkalinity is important for the initial formation of a population.
The analysis of the fractions of free and bound, liberated by hydrolysis, phenolic acids in the inflorescences and rhizomes with roots of Hieracium pilosella L. was conducted by 2D-TLC and RP-HPLC methods. In the inflorescences fourteen phenolic acids were found: chlorogenic, gallic, protocatechuic, homoprotocatechuic, caffeic, gentisic, p-hydroxyphenylacetic, p-hydroxybenzoic, m-hydroxybenzoic, p-coumaric, syringic, vanillic, ferulic and salicylic. In the rhizomes with roots nine phenolic acids were identified: chlorogenic, gallic, homoprotocatechuic, caffeic, gentisic, p- hydroxyphenylacetic, p-hydroxybenzoic, syringic and vanillic. By means of the RP-HPLC method the contents of major acids were estimated which ranged from 0.01 mg% up to 139.50 mg%. Caffeic acid is the remarkably dominating constituent of both plant materials. Phenolic compounds may contribute to invasiveness and medicinal properties of this species.
An ethanol extract of rhizomes of Potentilla erecta was investigated for its activity against the cariogenic bacterium Streptococcus sobrinus/downei CCUG 21020. The crude extract showed moderate antibacterial activity but strongly inhibited the total activity of streptococcal glucosyltransferases (GTFs) and their ability to synthesize water-insoluble glucan (mutan).
The fraction of sterols acetates from the herb and rhizomes from C. palustre have been isolated in the typical way from petroleum ether extract. By means of the weight method the total amounts of sterols was determined in herb (0.1192%), in rhizomes (0.0517%). The mixture of sterols acetates was investigated by capillary gas chromatography GC MS techniques. Stigmasterol, β-sitosterol, campesterol and ergosterol were identified in both fractions. Stigmasterol and β-sitosterol were the predominant sterol components in analysed raw materials from C. palustre.
Celem naszej pracy było określenie zawartości procentowej i składu chemicznego olejków eterycznych występujących w kwiatostanach, liściach i kłączach Silphium perfoliatum L. Zawartość procentową olejku oznaczano metodami destylacji z parą wodną w aparacie Derynga wg FP IV i FP V. Skład olejków eterycznych otrzymanych z kwiatostanów i liści jest podobny z tym, że w przypadku kwiatostanów dominującym związkiem w olejku jest a-pinen (20,9%), zaś w przypadku liści głównym składnikiem jest tlenek kariofilenu (34,7%). Natomiast skład olejków eterycznych otrzymanych z kłączy różni się istotnie od składu olejków otrzymanych z liści i kwiatostanów.
Polypodium vulgare L. rhizome cells tolerate water deficit stress to different degrees. This study examined the extent of ultrastructural changes in the endodermis and stelar elements in response to mannitol dehydration treatment. Cytological observations showed that the rhizomes possess structural adaptations to withstand drying by maintaining water inside the stele or activating mechanisms that mitigate stress. There are Casparian strips on the walls of the endodermis, and thicker cell walls of cortex parenchyma cells bordered with endodermal cells. Numerous electron-dense vesicles accumulate in dehydrated endodermal cells, making the organelles not visible. In parenchymatous cells of pericycle and vascular parenchyma cells, only nuclei with slightly condensed chromatin, smaller starch grains and vesicle formation were observed in the cytoplasm after dehydration. Changes in cell membrane ultrastructure were not identified. Incubation of the rhizome in abscisic acid prior to dehydration did not produce ultrastructural changes.
Do doświadczenia wykorzystano odmiany cantedeskii ‘Black Eyed Beauty’, ‘Cameo’ i ‘Treasure’. Do doniczek o średnicy 18 cm sadzono kłącza suche, moczone w wodzie oraz moczone przez 30 minut w kwasie giberelinowym o stężeniu 150 mg·dm⁻³. W każdym sposobie przygotowania kłączy badano efekt dolistnego zastosowania GA₃ Moczenie kłączy w kwasie giberelinowym zwiększyło plon kwiatów badanych odmian. Kwas giberelinowy opóźnił kwitnienie roślin o 1-2 tygodnie. Z kłączy moczonych w kwasie giberelinowym u odmian 'Black Eyed Beauty’ i ‘Cameo’ wyrosły kwiaty o najdłuższych szypułach kwiatostanowych, a u odmiany 'Treasure' - o najkrótszych. Kwas giberelinowy zastosowany do moczenia kłączy miał pozytywny wpływ na intensywność zazielenienia liści odmiany 'Treasure'.
Rhizomes of Zantedeschia, 15-18 cm in circumference with leaf buds 0.5-1 cm in length, were planted into 20-cm pots on 15 May 2006 into a medium consisting of peat with a pH of 6.2, enriched with a slow-release fertiliser Osmocote Plus (3-4M) and mixed with fresh shredded pine bark at a rate of 3:1 (v:v). Before planting, the rhizomes were soaked for 20 minutes in a water solution of gibberellic acid at a concentration of 150 mg x dm-3, or sprayed with the same concentration once, twice or three times, each time after thorough drying. For each round of spraying, 100 ml of the solution were used. Gibrescol 10 MG was applied. Gibberellic acid had a similar effect, whether applied in the form of a solution for soaking or spraying of rhizomes. The number of sprayings of rhizomes had no effect on the effectiveness of gibberellic acid. In the cultivar ‘Black Magic’, rhizome spraying caused earlier flowering, while in ‘Cameo’ a similar effect was obtained by soaking the rhizomes in gibberellic acid. Gibberellic acid had no effect on the quality of flowers and the number of leaves. Gibberellic acid had a good effect on the index of leaf greenness in the cultivars under study.
Pathogenicity and ultrastructural investigation of the inoculation of peppermint stems and rhizomes with Phoma strasseri conidia was undertaken using scanning and transmission electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. strasseri isolates had infected the stems and rhizomes of peppermint. Of all inoculation methods, direct placement of colonized agar plugs on damaged epidermis and soaking stems and rhizomes in conidial suspension were the most e#ective. !e behavior of the conidia deposited on the stems and rhizomes was investigated at different time intervals after inoculation: 6, 16, 24, 36 and 48 h. Conidia produced an appressorium directly at the end of a short germ tube. Appressoria were formed over the cuticle, but never over stomata. Direct penetration to host tissue through the cuticle was observed. The spore and hyphae were covered with a mucilaginous sheath.
Clonal plants combine sexual and clonal reproduction, which contribute differently to plant fitness. We investigated the consequence of natural selection on the two reproductive modes and the ecological factors that affect the reproductive modes of Eremosparton songoricum (Litv.) Vass. in order to understand the reproductive strategies of a clonal plant and the relationship between sexual and clonal reproduction. Morphological characteristics and biomass allocation patterns were m easured and compared at the clonal fragment level in two natural populations (riverside population A and hinterland population B) in the Gurbantunggut Desert, China. The flowering plant ratio, inflorescence number per plant, flower number per raceme, fruit number, mass of 100 seeds, root biomass, fruit biomass and ramet height were significantly higher, whereas fruit set, ramet density, rhizome biomass and length, and root number were significantly lower, in population A compared with those in population B. We estimated that the importance of clonal reproduction varies in two populations of E. songoricum, and the investment adjustment in the two reproductive modes may be based on cost required for each new plant to generate the more survival progeny in the plant life time.
A systematic knowledge of clonal integration is an important step in understanding the ecological implication of clonality. This study focuses on the performance of rhizomatous clonal plants under different situations and we proposed a hypothesis that clonal integration will significantly improve the disturbance and drought resistance ability and the competitive ability of Eremosparton songoricum. In 2009, the experiments were carried out in two natural populations. Rhizome was either severed (S) or not (I) in four treatments that include control (C), drought (D), disturbance (E), and competition (F). The biomass and the root-shoot ratio were compared in different experimental treatments. Under drought and disturbance treatments, the biomass of ramet with severed rhizome was significantly less than that of intact ramets, and both were lower than the samples under the control treatment. The differences in root-shoot ratio were opposite to the biomass in drought and disturbance treatments. The ramet biomass under the competition treatment had the same result as that under the drought and disturbance treatments. However, th e root-shoot ratio was highest in FS (competition treatment with severed rhizome) and lowest in FI (competition treatment with intact rhizome) under competition and control treatments. Our results suggest that clonal integration enhances the disturbance and drought resistance ability rather than the competitive ability of Eremosparton songoricum. This may be one of the various reasons why E. songoricum is distributed in sand dunes of droughty conditions with more disturbances but less competition. Integration proved to be important for the species occupying adverse patches. For E. songoricum, the existence of rhizome reduces the impact of environmental stress and improves the fitness in association with its location at the dune.
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