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The aim of the work was to analyse differentiation of calcium and dairy products intake by older women in a retrospective analysis, taking into consideration place of residence. The study was carried out in two stages in years 1999 and 2004 among 192 women aged 75-80 living in the city, town and country. In the first stage, nutrition manner was evaluated, using the 24-h recall method. After 5 years data of the analysed women mortality was gathered. Differences in nutrients and dairy products intake were analysed between two groups: alive and dead women, taking into consideration place of residence. Our results give sufficient grounds for regarding older women living in towns of Olsztyn region as a group with an elevated risk of all-cause mortality. The analysis carried out separately for each place of living (city – town – country) showed no correlation between all-cause mortality and the intake of calcium and dairy products in the 5-year retrospective study. The revealed tendencies indicate the need of continuing studies with longer exposition to nutrition factors.
The phenotypes of CAD related to arterial hypertension co-occurrence were analysed in 174 male patients and 117 control men for the associations with the polymorphisms of the MTHFR gene (677C>T and 1298A>C) and the PON1 gene (-108C>T) in relation to age at diagnosis (less or equal and more than 50 years). We noted the increased frequency of the three MTHFR genotypes: CC/AC, CT/AA and CC/CC in the CAD group (65.5%) in comparison to the control group (45.3%), corresponding to the 2.3-fold increased risk of CAD for men with these genotypes (95%CI (1.4-3.7); p=0.0005). The higher increase in risk of CAD was noted for the younger men (OR=3.6; 95%CI(1.6-8.3); p=0.002) and lower for the older (OR=1.8; 95%CI(1.0-3.4); p=0.03). In the normotensive men the greater impact on CAD risk had the homozygous genotypes; the 2.3-fold higher risk was associated with MTHFR CC/AC, CC/CC and TT/AA genotypes (95%CI(1.2-4.4); p=0.01). After adjustment for age, the association between CAD and MTHFR was significant only for the younger normotensive men (OR=2.8; 95%CI (1.0-8.0); p=0.04). Additionally, we found that the younger part of the control group was characterized by higher frequency of the low expression PON1 -108T allele and PON1 -108TT genotype (0.54 and 31.9% respectively) in comparison to the older men (0.41 and 17.1% respectively; p=0.03).
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