Wyniki wyszukiwania

1

Scanning of Geotrichum candidum genome by RFLP-PCR of rDNA and RAPD

100%

Piegza M., Barszczewski W., Witkowska D., Stempniewicz R., Robak M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2006

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tom 09

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nr 3

2

In vitro amplification of the alpha-1-antitrypsin gene for detection of Z mutation

100%

Kowalska A., Schwartzman A. L., Rujner J., Kraszewski A., Gaitkhoki V. S.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1991

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tom 39

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nr 3

3

Genetic diversity of Trichomonas vaginalis clinical isolates according to restriction fragment length polymorphism analysis of the 60-kDa proteinase gene

100%

Hernandez H., Fraga J., Marcet R., Alba A., Figueredo M., Alfonso Y., Rojas L., Sarracent J.

Acta Parasitologica

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2019

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tom 64

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nr 2

4

Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

100%

Cetinkaya H., Haktanir D., Arun S., Vurusaner C.

Acta Parasitologica

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2016

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tom 61

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nr 1

5

FASN gene polymorphism in indigenous cattle breeds of Turkey

100%

Oztabak K., Gursel F. E., Akis I., Ates A., Yardibi H., Turkey G.

Folia Biologica

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2014

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tom 62

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nr 1

6

New polymorphism of FASN gene in chicken

100%

Marrube G., Rozen F., Pinto G. B., Pacienza N., Melo J. E., Huguet M. J., Canet Z., Zandomeni R., Miquel M. C.

Journal of Applied Genetics

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2004

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tom 45

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nr 4

7

Structure of the c-Ha-ras-1 locus in human breast tumors

84%

Mackiewicz M., Choina B., Kadlof A., Szala s.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1987

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tom 35

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nr 10-12

8

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

84%

Silva F. M. P., Lopes R. S., Bresciani K. D. S., Amarante A. F. T., Araujo Jr J. P.

Acta Parasitologica

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2014

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tom 59

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nr 1

In this paper we report the occurrence of zoonotic species of Cryptosporidium (C. ubiquitum and C. parvum subtype IIaA15G2R1) and the host-specific Giardia duodenalis genotype E in a sheep farm from Brazil. Fecal specimens were collected from 100 animals and screened for the presence of G. duodenalis and Cryptosporidium spp. by enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequence analysis of selected samples was used to confirm the species and to characterize the isolates. Of 100 specimens, 34 were positive for Giardia (34%) and 25 were positive for Cryptosporidium (25%). The prevalence of Giardia and Cryptosporidium was higher in lambs than in ewes. Co-infection was observed in 14% of animals. All G. duodenalis isolates belonged to genotype E. The genetic characterization performed in 22 Cryptosporidium-positive samples revealed the presence of zoonotic C. ubiquitum and C. parvum in 76% (19/25) and 12% (3/25) of infected animals, respectively. The presence of zoonotic Cryptosporidium subtypes in lambs was of epidemiological and public health relevance.

9

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Comparison of physiological and PCR-RFLP rDNA identification of yeast species commonly found in cheese

84%

Polomska X., Juszczyk P., Cadez N., Raspor P., Robak M., Wojtatowicz M.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 2

221-226

The identification of twenty four yeast isolates from cheese performed with API ID 32C simplified assimilation test and restriction fragments length polymorphism (PCR-RFLP) of ITS1-5.8S rDNA-ITS2 region was compared. The accordance of the results of both methods for eleven strains identified as Candida famata, five strains as C. lipolytica and four strains as C. sphaerica was observed. However, the other four yeast isolates classified with API as C. sphaerica revealed amplification products as well as restriction patterns characteristic for C. famata species.

10

Application of non-radioactive methods of DNA detection in analysis of human genetic disorders

84%

Slomski R., Reiss J., Jungerman M.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

11

Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles

84%

Rohilla M. S., Tiwari P. K.

Journal of Applied Genetics

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2008

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tom 49

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nr 2

167-182

DNA-based identification of species for phylogenetic analysis as well as forensic identification is widely being carried out with the help of polymerase chain reaction (PCR). In this study, a successful effort has been made to identify 5 species of Indian freshwater turtles, including 3 hard-shell turtles (Geoemydidae), i.e. Kachuga dhongoka, K. kachuga and Geoclemys hamiltoni, and 2 species of soft-shell turtles (Trionychidae), i.e. Aspideretes gangeticus and Lissemys punctata punctata, by using a well-optimized PCR-RFLP method. The analysis of nucleotide sequence variations in the PCR-amplified mitochondrial cyt-b genes (encoding cytochrome b) from the 5 species revealed its usefulness in the taxonomic differentiation of these species. On the basis of cyt-b sequence data and the PCR-RFLP pattern, a phylogeny was developed to resolve the genetic relationships between these species, living in the same habitat type. In comparison, the PCR-RFLP of mitochondrial 16S rDNA genes appeared less decisive in analysing phylogenetic relationships or even in species differentiation. Further, the molecular method (PCR-RFLP) developed here is simple, rapid, reliable and reproducible; hence it can be routinely applied for species identification, essential for conservation and management of endangered chelonian species.

12

IS6110-based differentiation of Mycobacterium tuberculosis strains

84%

Zaczek A., Ziolkiewicz M., Wojtasik A., Dziadek J., Sajduda A.

Polish Journal of Microbiology

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2013

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tom 62

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nr 2

In this study, 62 Mycobacterium tuberculosis strains were characterized by fast ligation-mediated PCR (FLiP) and, previously performed, IS6110 restriction fragment length polymorphism (RFLP). FLiP proved a reproducible and specific method for differentiation between M. tuberculosis strains. The discriminatory power of FLiP was close to that of the reference IS6110 RFLP suggesting its usefulness in studying the genetic diversity of M. tuberculosis strains.

13

Cutaneous leishmaniasis due to three Leishmania species among Syrian refugees in Sanliurfa, Southeastern Turkey

84%

Doni N. Y., Gurses G., Dikme R., Aksoy M., Zeyrek F. Y., Simsek Z., Satoskar A. R., Varikuty S., Yesilova Y.

Acta Parasitologica

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2020

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tom 65

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nr 4

14

Novel SNP of the goat prolactin gene [PRL] associated with cashmere traits

84%

Lan X.-Y., Pan C.-Y., Chen H., Lei C.-Z., Li F.-Y., Zhang H.-Y., Ni Y.-S.

Journal of Applied Genetics

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2009

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tom 50

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nr 1

51-54

Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goat PRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed by Eco241 PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P = 0.014).

15

The molecular diversity of different isolates of Beauveria bassiana [Bals.] Vuill. as assessed using inter-microsatellites [ISSRs]

84%

Estrada M. E., Camacho M. V., Benito C.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 2

Inter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

16

A restriction polymorphism of mitochondrial gene orf25 in selected species of rye

84%

Skuza L., Rogalska S. M.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

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2005

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tom 72

17

Screening of chromosomal region 21q22.3 for mutations in genes associated with neuronal Ca2plus signalling in bipolar affective disorder

84%

Kostyrko A., Hauser J., Rybakowski J. K., Trzeciak W. H.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

The therapeutic effect of lithium in bipolar affective disorder may be connected with decreasing intracellular Ca2+ concentrations. Several linkage studies have identified a potential bipolar affective disorder susceptibility locus within chromosomal region 21q22.3. This locus contains two genes expressed in the brain - ADARB1 and TRPM2 - involved in regulating intracellular Ca2+ concentrations. The aim of this study was an identification of mutations in the coding sequences of ADARB1 and TRPM2 and their association with bipolar affective disorder. For that purpose we screened 60 patients with bipolar affective disorder and a control group of 66 subjects using single strand conformation polymorphism and sequence analysis. For rapid screening we performed restriction fragment length polymorphism analysis. Screening of bipolar affective disorder patients for mutations in TRPM2 led to identification of three novel and four known transitions. Two transitions resulted in the substitutions: R755C and A890V. Screening of the coding sequence of ADARB1 did not reveal any mutations except one already known transition. A comparison of the transition frequency in patients and controls does not support association of the detected mutations with bipolar affective disorder. According to our results, bipolar affective disorder may not be caused by mutations in ADARB1. However, this study does not exclude TRPM2 as a candidate gene since we have screened only about 30 per cent of the entire coding sequence of this large gene.

18

Comparison of DNA-based typing methods of microbial organisms for epidemiological studies

84%

Kur J., Krawczyk B.

Advances in Agricultural Sciences

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2002

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tom 08

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nr 1

19

Amplification of a single-locus variable-number direct repeats with restriction fragment length polymorphism [DR-PCR-RFLP] for genetic typing of Acinetobacter baumannii strains

84%

Nowak-Zaleska A., Krawczyk B., Kotlowski R., Mikucka A., Gospodarek E.

Polish Journal of Microbiology

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2008

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tom 57

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nr 1

In search of an effective DNA typing techniaue for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target seauence was evaluated. Using known genomic seauences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment length polymorphism (DR-PCR/RFLP) method. A total of 90 Acinetobacter baumannii strains isolated from patients of the Clinical Hospital in Bydgoszcz, Poland, were examined. Initially, all strains were typed using macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis (REA-PFGE). Digestion of the chromosomal DNA with the Apal endonuclease and separation of the fragments by PFGE revealed 21 uniaue types. Application of DR-PCR/RFLP resulted in recognition of 12 clusters. The results showed that the DR-PCR/RFLP method is less discriminatory than REA-PFGE, however, the novel genotyping method can be used as an alternative techniaue for generating DNA profiles in epidemiological studies of intra-species genetic relatedness of Acinetobacter baumannii strains.

20

Identification of ectomycorrhizal fungi isolated from roots of birch growing on metallurgic heap

84%

Krupa P., Piotrowska-Seget Z.

Polish Journal of Ecology

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2004

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tom 52

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nr 3

Protective role of mycorrhizal fungus for plants against contamination with heavy metals makes a possibility to use fungi inocula for recultivation areas devastated by industry. Use of mycorrhizal symbiont for improving the living conditions of trees requires unambiguous identification of fungi that are able to form successful mycorrhizae in contaminated areas. We took up studies on identification of fungal species, which create mycorrhizal symbiosis with roots of birch growing on metallurgic heap. The identification of fungi collected directly from roots was performed by comparing patterns of restriction fragments of the fungal ITS products with DNA pattern obtained from fruiting bodies of Basidiomycetes fungi growing in the study area.

Ograniczanie wyników

Scanning of Geotrichum candidum genome by RFLP-PCR of rDNA and RAPD

In vitro amplification of the alpha-1-antitrypsin gene for detection of Z mutation

Genetic diversity of Trichomonas vaginalis clinical isolates according to restriction fragment length polymorphism analysis of the 60-kDa proteinase gene

Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

FASN gene polymorphism in indigenous cattle breeds of Turkey

New polymorphism of FASN gene in chicken

Structure of the c-Ha-ras-1 locus in human breast tumors

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

Comparison of physiological and PCR-RFLP rDNA identification of yeast species commonly found in cheese

Application of non-radioactive methods of DNA detection in analysis of human genetic disorders

Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles

IS6110-based differentiation of Mycobacterium tuberculosis strains

Cutaneous leishmaniasis due to three Leishmania species among Syrian refugees in Sanliurfa, Southeastern Turkey