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Restriction enzyme analysis of the capsid protein gene of different strains of European brown hare syndrome virus

100%

Chrobocinska M.

Bulletin of the Veterinary Institute in Puławy

2001

tom 45

nr 2

Eight Polish strains of European brown hare syndrome virus, isolated in 1992-93, 1998 and 2001, as well as 2 standard strains: French (F/st) and Italian (It/st) were analysed. The fragments of genome, including the capsid protein gene, were amplified by RT-PCR or N-PCR and digested by endonucleases: Xho I, Sst I, BamH I, Sma I, Hpa II and BsuR I. The results of restriction enzyme digestion were compared with restriction maps, obtained after computer analysis of nucleotide sequences of other European strains, available in GenBank. The results of analysis of Polish strains, isolated in different years, revealed the changes in restriction profiles between them and other European strains. The 3 out of 5 Polish strains detected in 1992-93 demonstrated the same restriction profiles, similar to French "GD" strain. The two other strains revealed, for same endonucleases, restriction patterns similar to ltalian "BS89" or German strains. The strains isolated in 1998 and 2001 demonstrated higher differences in restriction profiles in comparison with Polish and other European strains previously detected. These changes in nucleotide scquences of the part of genome studied, indicate the diversity of EBHSV.

Genetic profiles of bovine herpesvirus 1 strains isolated from cattle in Poland

84%

Rola J., Zmudzinski J.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 4

The purpose of the study was to determine subtypes of BHV1 strains isolated from cattle in Poland. In total five different strains of BHV1 were isolated during the last years. All isolates as well as four archival and two reference BHV1 strains were analysed by PCR and restriction enzyme analysis. Specificity of the strains was confirmed by PCR with primers complementary to the nucleotide sequence of gD gene. Subsequently, genomic DNA of the tested strains was digested with endonucleases Hind III and Hpa I. Restriction enzyme analysis revealed that all recently isolated BHV1 strains belonged to the BHV1.1 subtype. Among archival viruses, two strains had restriction pattern similar to the subtype BHV1.1 and two others to the subtype BHV1.2a. The presented study showed that currently subtype BHV1.1 is dominant in cattle population in Poland.

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

1 Chrobocinska M.

1 Rola J.

1 Zmudzinski J.

1 2010

1 2001

Wyniki wyszukiwania

Restriction enzyme analysis of the capsid protein gene of different strains of European brown hare syndrome virus

Genetic profiles of bovine herpesvirus 1 strains isolated from cattle in Poland