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1
Content available remote

Altered expression of immune surface markers in children with recurrent infections of respiratory tract

100%
Wasik M., Kaczorowska M., Demkow U.
Journal of Physiology and Pharmacology. Supplement
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2005
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tom 56
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nr 4
237-243
Infections of the respiratory tract are very common in young children. They may affect the quality of life and have a great economic impact. On the other hand, respiratory tract infections through MALT system play a positive role in the maturation and development of the immune system. The aim of the study was to examine quantitative and qualitative changes of T and B cells and granulocytes surface molecules in 24 children with recurrent (more than 8 episodes per year) infections of the respiratory tract and in 18 healthy children. The expression of CD2, CD3, CD4, CD8 on T cells; HLA-DR, CD19, CD5/CD20 on B cells, and CD11a/CD18, CD11b/CD18, CD62L, CD16 on granulocytes from peripheral blood was evaluated by a flow cytometry method. We observed a significant increase in the CD5+/CD20+ positive cells on B cells. We also observed a decreased expression of CD11c+/CD18+ cells, CD11a, and CD62L on granulocytes. The expression of other structures on lymphocytes and the CD11b/CD18+ on granulocytes remained unchanged. CD5+/CD20+ cells constitute a filogenetically old population responsible for the production of IgM of low specificity and affinity for specific antigens. The prevalence of this fetal phenotype population may be explained as a delayed maturation of the humoral immune system leading to increased susceptibility to infections. A lower percentage of CD11a may be related to the blockade of that molecule by rhinoviruses.
2

Antimicrobial resistance in Rhodococcus equi

100%
Cisek A. A., Rzewuska M., Witkowski L., Binek M.
Acta Biochimica Polonica
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2014
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tom 61
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nr 4
3
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Empirical first-line antibioticotherapy in adult rural patients with acute respiratory tract infections

100%
Panasiuk L., Lukas W., Paprzycki P.
Annals of Agricultural and Environmental Medicine
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2007
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tom 14
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nr 2
The objective of the study was the evaluation of the frequency of occurrence of acute respiratory infections among rural population from the Lublin Region, and verifi cation of the appropriateness of the use of antibiotics in empirical fi rst-line therapy of these diseases. The analysis covered medical records concerning 1,839 patients selected from 27 rural health centres within the period of one year (1 September 2005–31 August 2006). As many as 656 patient visits were recorded, the reasons for which were qualifi ed by physicians as infections of the upper and lower airways. These disorders were diagnosed in 450 patients, which constituted 24.46% of the population examined. The following diagnoses were most frequently made: acute pharyngitis and tonsillitis (31.25% of the total number of diagnoses), acute upper respiratory infections of multiple and unspecifi ed sites (22.25%), and common cold (21.64%). Antibiotics were used in empirical fi rst-line therapy in as many as 82% of the total number of patients. The analysis showed that in the great majority of cases the use of antibiotics was inappropriate. If this situation persists, in the near future it will lead to an increase in the phenomenon of antibioticoresistance in Poland. In order to prevent this situation, it is necessary to carry out an extensive educational action concerning the hazardous effect of excessive use of antibiotics, both among physicians and the whole society.
4

Infections caused by RSV among children and adults during two epidemic seasons

84%
Pancer K., Ciacka A., Gut W., Lipka B., Mierzejewska J., Milewska-Bobula B., Smorczewska-Kiljan A., Jahnz-Rozyk K., Dzierzanowska D., Madalinski K., Litwinska B.
Polish Journal of Microbiology
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2011
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tom 60
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nr 3
Respiratory Syncytial Virus (RSV) is one of the most common causes of lower respiratory tract infections in young children, immunocompromised patients (children and adults), patients with chronic respiratory diseases and elderly people. Reinfections occur throughout the life, but the severity of disease decreased with subsequent infection. The aim of this study was to analyze the frequency of RSV infections in two selected subpopulations: young children (below 5 y.) and adults with chronic respiratory diseases (25–87 y.). Nasopharyngeal swabs (334) collected from October 2008 to March 2010 were examined. The presence of RSV genome was determined by RT-PCR and the presence of RSV antigen by quick immunochromatographic test. Positive results of RT-PCR were found in 45.2% of all swabs: 48.6% samples in 2008; 41.5% in 2009; 50.8% in 2010. The highest frequency of RSV-positive samples was in fall-winter months, but differences in RSV epidemic seasons were found. In the first season (2008–2009) an increased number of RSV infections was observed from November 2008, but in the second season – from January 2010. Generally, the frequency of RSV-positive RT-PCR among children was 53%, among adults 25%. The highest difference was observed in the first three-month period of 2010. RT-PCR positive samples were found in 68.5% of children and 5.9% of adults. However, the RSV antigen was found in 44.4% of samples collected from adults in this period. Our results indicate that the contribution of RSV infections during epidemic season of respiratory tract infections in Poland was really high among children and adults.
5
Content available remote

Tussigenic agents in the measurement of cough reflex sensitivity

84%
Pecova R., Javorkova N., Kudlicka J., Tatar M.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
|
nr 5
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture

84%
Cymerys J., Dzieciatkowski T., Slonska A., Bierla J., Tucholska A., Chmielewska A., Golke A., Banbura M. W.
Polish Journal of Veterinary Sciences
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2010
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tom 13
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nr 4
Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
7

European and Asian species of Trollius L.

84%
Witkowska-Banaszczak E., Bylka W.
Herba Polonica
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2007
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tom 53
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nr 2
8

The effect of the 162 km endurance ride on equine peripheral blood neutrophil and lymphocyte functions

67%
Cywinska A., Wyszynska Z., Gorecka R., Szarska E., Witkowski L., Dziekan P., Winnicka A., Schollenberger A.
Polish Journal of Veterinary Sciences
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2010
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tom 13
|
nr 2
9

Anaerobic bacteria colonizing the lower airways in lung cancer patients

67%
Rybojad P., Los R., Sawicki M., Tabarkiewicz J., Malm A.
Folia Histochemica et Cytobiologica
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2011
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tom 49
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nr 2
10

The cystic fibrosis neutrophil: a specialized yet potentially defective cell

67%
Hayes E., Pohl K., McElvaney N. G., Reeves E. P.
Archivum Immunologiae et Therapiae Experimentalis
|
2011
|
tom 59
|
nr 2
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