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The detection and purification of a protein that recognizes a line 1 DNA repetitive sequence

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Lukyanov D., Rogolinski J., Podgornaya O., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

2000

tom 05

nr 4

551-521

A protein which binds specifically to MspI8 (a 454 bp long repeated sequence highly homologous to the 5’ untranslated region (5'UTR) of the LINE 1 sequence) was found and identified in nuclear extracts of rat liver cells. This protein was detected using the electrophoretic mobility shift assay (EMSA) and was purified by Q-Sepharose and DNA affinity chromatography. Its molecular mass was estimated by SDS electrophoresis as 29 kDa. The possibility that this protein (p29) is the rat analogue of human L1PBP-A, specific for the human LINE sequence LPE1, is discussed.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Lukyanov D.

1 Podgornaya O.

1 Rogolinski J.

1 Rzeszowska-Wolny J.

1 2000

Wyniki wyszukiwania

The detection and purification of a protein that recognizes a line 1 DNA repetitive sequence