Wyniki wyszukiwania

1

In vitro reoxygenation following hypoxia increases MMP-2 and TIMP-2 secretion by human umbilical vein endothelial cells

100%

Cavdar Z., Oktay G., Egrilmez M. Y., Genc S., Genc K., Altun Z., Islekel H., Guner G.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

69-73

Endothelial cells lining the inner blood vessel walls play a key role in the response to hypoxia, which is frequently encountered in clinical conditions such as myocardial infarction, renal ischemia and cerebral ischemia. In the present study we investigated the effects of hypoxia and hypoxia/reoxygenation on gelatinases (matrix metalloproteinase-2 and -9), their inhibitor (TIMP-2) and activator (MT1-MMP), in human umbilical vein endothelial (HUVE) cells. HUVE cells were subjected to 4 h of hypoxia or hypoxia followed by 4 and 24 h of reoxygenation. The pro- and active forms of MMP-2 and MMP-9 were analyzed by gelatin zymography; TIMP-2 protein level was assayed using ELISA, while MT1-MMP activity was measured using an activity assay. The secretion of MMP-2 proform increased significantly in cells subjected to 4h of hypoxia followed by 4 or 24 h of reoxygenation, compared with the normoxic group. TIMP-2 protein level also increased significantly in the hypoxia/reoxygenation groups, compared with the normoxic group. There were no statistically significant differences in the levels of active MT1-MMP in all groups. This study indicates that MMP-2 and TIMP-2 could be regarded as important components of a mechanism in the pathophysiology of ischemic injury following reperfusion.

2

MiR-378a-5p regulates CAMKK2/AMPK pathway to contribute to cerebral ischemia/reperfusion injury-induced neuronal apoptosis

100%

Zhang Y., Zhang P., Deng C.

Folia Histochemica et Cytobiologica

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2021

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tom 59

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nr 1

3

Ageing influences the effect of pre-hypoxic administration of clonidine, an alpha2-adrenoceptor agonist, on post-hypoxic vasomotoricity

84%

Gourdin M. J., Ponchau O., Jamart J., De Kock M.

Journal of Physiology and Pharmacology

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2012

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tom 63

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nr 2

4

Changes of mitochondrial pathway in hypoxia-reoxygenation induced cardiomyocytes apoptosis

84%

He A., Wang J., Gui C., Jiang Y., Sun Y., Chen T.

Folia Histochemica et Cytobiologica

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2007

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tom 45

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nr 4

397-400

5

p21WAF1 and hypoxia/reoxygenation-induced premature senescence of H9c2 cardiomyocytes

67%

Wang D., Zhang Y.-Z., Yang B., Zhang F.-X., Cao M.-Y., Wang C., Chen M.-L.

Folia Histochemica et Cytobiologica

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2011

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tom 49

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nr 3

Ograniczanie wyników

In vitro reoxygenation following hypoxia increases MMP-2 and TIMP-2 secretion by human umbilical vein endothelial cells

MiR-378a-5p regulates CAMKK2/AMPK pathway to contribute to cerebral ischemia/reperfusion injury-induced neuronal apoptosis

Ageing influences the effect of pre-hypoxic administration of clonidine, an alpha2-adrenoceptor agonist, on post-hypoxic vasomotoricity

Changes of mitochondrial pathway in hypoxia-reoxygenation induced cardiomyocytes apoptosis

p21WAF1 and hypoxia/reoxygenation-induced premature senescence of H9c2 cardiomyocytes