Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: regulatory element

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Regulation of alternative splicing by RNA secondary structure

100%

Lisowiec J., Dominiak G., Kierzek R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2014

tom 95

nr 3

Regulation of protamine gene expression in an in vitro homologous system

84%

Jankowski J. M., Cannon P. D., Van der Hoorn F., Wasilewska L. D., Wong N. C. W., Dixon G. H.

Acta Biochimica Polonica

1996

tom 43

nr 2

An in vitro transcription system from the trout testis nuclei was developed to study trout protamine gene expression. The protamine promoter contains, among others, two regulatory elements: 1) a cAMP-responsive element or CRE element (TGACGTCA) which is present in position 5' to TATA box, and 2) GC box (CCGCCC) which is present in position 3' to TATA box. The removal of the CRE-binding protein by titration (by the addition of appropriate oligonucleotides to the incubation mixture) resulted in a decrease in transcription of the protamine gene. These results were confirmed by experiments in which the pure CRE-binding factor (TPBP1) was used, as well as by those where a stimulatory effect of cAMP on protamine promoter transcription was observed. On the other hand, addition of oligonucleotides containing the GC-box sequence enhanced the protamine gene transcription indicating that the protein (Spl like) which binds to this sequence acts as a repressor of protamine gene expression. These results confirm the previously proposed model which suggested that the GC box played a role in negative regulation of the protamine gene expression. Involvement of some other factors in this process was also discussed.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Cannon P. D.

1 Dixon G. H.

1 Dominiak G.

1 Jankowski J. M.

1 Kierzek R.

1 Lisowiec J.

1 Van der Hoorn F.

1 Wasilewska L. D.

1 Wong N. C. W.

1 2014

1 1996

Wyniki wyszukiwania

Regulation of alternative splicing by RNA secondary structure

Regulation of protamine gene expression in an in vitro homologous system