Wyniki wyszukiwania

1

Polyamines as regulators of cell activation

100%

Kaminska B.

Acta Biochimica Polonica

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1993

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tom 40

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nr 3

2

Isolation and expression pattern of RGS21 gene, a novel RGS member

88%

Li X., Chen L., Ji C., Liu B., Gu J., Xu J., Zou X., Gu S., Mao Y.

Acta Biochimica Polonica

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2005

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tom 52

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nr 4

Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein α subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of αi/o family members and/or αq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.

3

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Optimizacija raboty dizelejj sel'skokhozjaistvennykh mashin i traktorov

75%

Chekmenev V.

Motrol. Motoryzacja i Energetyka Rolnictwa

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2015

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tom 17

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nr 1

4

Regulator of G-protein signalling expression and function in ovarian cancer cell lines

75%

Hurst J. H., Mendpara N., Hooks S. B.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 1

153-174

Regulator of G-protein signalling (RGS)2 proteins critically regulate signalling cascades initiated by G-protein coupled receptors (GPCRs) by accelerating the deactivation of heterotrimeric G-proteins. Lysophosphatidic acid (LPA) is the predominant growth factor that drives the progression of ovarian cancer by activating specific GPCRs and G-proteins expressed in ovarian cancer cells. We have recently reported that RGS proteins endogenously expressed in SKOV-3 ovarian cancer cells dramatically attenuate LPA stimulated cell signalling. The goal of this study was twofold: first, to identify candidate RGS proteins expressed in SKOV-3 cells that may account for the reported negative regulation of G-protein signalling, and second, to determine if these RGS protein transcripts are differentially expressed among commonly utilized ovarian cancer cell lines and non-cancerous ovarian cell lines. Reverse transcriptase-PCR was performed to determine transcript expression of 22 major RGS subtypes in RNA isolated from SKOV-3, OVCAR-3 and Caov-3 ovarian cancer cell lines and non-cancerous immortalized ovarian surface epithelial (IOSE) cells. Fifteen RGS transcripts were detected in SKOV-3 cell lines. To compare the relative expression levels in these cell lines, quantitative real time RT-PCR was performed on select transcripts. RGS19/GAIP was expressed at similar levels in all four cell lines, while RGS2 transcript was detected at levels slightly lower in ovarian cancer cells as compared to IOSE cells. RGS4 and RGS6 transcripts were expressed at dramatically different levels in ovarian cancer cell lines as compared to IOSE cells. RGS4 transcript was detected in IOSE at levels several thousand fold higher than its expression level in ovarian cancer cells lines, while RGS6 transcript was expressed fivefold higher in SKOV-3 cells as compared to IOSE cells, and over a thousand fold higher in OVCAR-3 and Caov-3 cells as compared to IOSE cells. Functional studies of RGS 2, 6, and 19/GAIP were performed by measuring their effects on LPA stimulated production of inositol phosphates. In COS-7 cells expressing individual exogenous LPA receptors, RGS2 and RSG19/GAIP attenuated signalling initiated by LPA1, LPA2, or LPA3, while RGS6 only inhibited signalling initiated by LPA2 receptors. In SKOV-3 ovarian cancer cells, RGS2 but not RGS6 or RGS19/GAIP, inhibited LPA stimulated inositol phosphate production. In contrast, in CAOV-3 cells RGS19/GAIP strongly attenuated LPA signalling. Thus, multiple RGS proteins are expressed at significantly different levels in cells derived from cancerous and normal ovarian cells and at least two candidate RGS transcripts have been identified to account for the reported regulation of LPA signalling pathways in ovarian cancer cells.

5

Conception of the small-pieces building production’ automation

63%

Ulshin V., Zubov D., Gorbunov A.

Teka Komisji Motoryzacji i Energetyki Rolnictwa

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2010

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tom 10B

6

The cystic fibrosis neutrophil: a specialized yet potentially defective cell

51%

Hayes E., Pohl K., McElvaney N. G., Reeves E. P.

Archivum Immunologiae et Therapiae Experimentalis

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2011

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tom 59

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nr 2

7

Optimisation of parameters of diesel’s regulator and loading

51%

Yusupov R. C., Deev V. Y., Zaynishev A. V., Solomonenko M. V.

Teka Komisji Motoryzacji i Energetyki Rolnictwa

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2003

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tom 03

8

Structure of small G proteins and their regulators

51%

Paduch M., Jelen F., Otlewski J.

Acta Biochimica Polonica

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2001

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tom 48

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nr 4

In recent years small G proteins have become an intensively studied group of regulatory GTP hydrolases involved in cell signaling. More than 100 small G proteins have been identified in eucaryotes from protozoan to human. The small G protein superfamily includes Ras, Rho Rab, Rac, Sar1/Arf and Ran homologs, which take part in numerous and diverse cellular processes, such as gene expression, cytoskeleton reorganization, microtubule organization, and vesicular and nuclear transport. These proteins share a common structural core, described as the G domain, and significant sequence similarity. In this paper we review the available data on G domain structure, together with a detailed analysis of the mechanism of action. We also present small G protein regulators: GTPase activating proteins that bind to a catalytic G domain and increase its low intrinsic hydrolase activity, GTPase dissociation inhibitors that stabilize the GDP-bound, inactive state of G proteins, and guanine nucleotide exchange factors that accelerate nucleotide exchange in response to cellular signals. Additionally, in this paper we describe some aspects of small G protein interactions with downstream effectors.

9

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I-IGFBP expression pattern

51%

Abdallah B. M.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 4

Insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs) are essential regulators for osteoblast proliferation and differentiation. It has been reported that Dexamethasone (Dex), an active glucocorticoid (GC) analogue, synergizes the stimulatory effect of 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) on osteoblast differentiation in the mouse fibroblastic cell line NIH3T3. I investigated whether this stimulatory effect is associated with changes in the expression pattern of the IGF/IGFBP system. Quantitative real-time PCR technology was used to quantify the gene expression levels of the IGF-system during osteoblast differentiation and in response to 1,25(OH)(2)D(3) or Dex alone under serum-containing and serum-free culture conditions. Interestingly, NIH3T3 was shown to express high mRNA levels of IGF-I, IGF-II and IGFBP-5, and low levels of both IGFBP-2 and-6. During osteoblast differentiation (days 6-12), IGF-I mRNA was repressed by more than 60%, while the transcript of IGFBP-5 was markedly up-regulated, by more than 50-fold. Similarly, treatment with Dex alone resulted in a dose-and time-dependent increase in the expression of IGFBP-5 and a decrease in IGF-I mRNA. Treatment with 1,25(OH)(2)D(3) alone increased the mRNA levels of IGF-I and IGFBP-6 by around 4-and 7-fold, respectively, in a dose-and time-dependent manner. In conclusion, my data demonstrated that osteoblast differentiation of NIH3T3 is associated with changes in the expression pattern of IGFs/IGFBPs, which are regulated by glucocorticoid in the presence of 1,25(OH)(2)D(3). Modulation of the IGF/IGFBP levels by glucocorticoid might suggest important roles for the IGF-system in mediating the osteoblast differentiation of the NIH3T3 cell line.

10

Effects of different energy levels and ambient temperatures on serum thyroid hormones and some biochemical values in Holstein steers

51%

Guler T., Kul S., Ertas O. N.

Medycyna Weterynaryjna

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2006

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tom 62

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nr 01

This 2x2 factorial study was designed to test the effects of different diet energy levels (Low: 5.39 NEf MJ/kg and High: 6.60 NEf MJ/kg) and environmental temperature (Low: 19.84°C and High: 28.5°C) on triiodothyronine (T₃), thyroxine (T₄) and blood serum parameters of Holstein steers. A total of 28 10-month old steers were divided into 4 groups, each containing 7 steers. According to this design, steers which were subjected to housing in barns were either offered normal energy (Barn-NE group) or high energy (Barn-HE group) diets; steers which were subjected to housing outside were also either offered normal energy (Outside-NE group) or high energy (Outside-HE group) diets. The highest levels of T₃ and T₄ were in the Outside-HE group followed by Outside-NE group, Barn-HE group and Barn-NE group, respectively (p<0.05). Serum glucose, cholesterol and triglyceride levels were higher in all the outside groups (p<0.05). No differences were found between the groups in the total protein, albumin, uric acid, inorganic phosphor, Ca, Na and K levels. The levels of SCOT, SGPT, amylase, alkaline phosphates and creatinin were similar in all groups. The results of the study indicated that increasing energy levels and ambient temperatures increased T₃ and T₄ levels and did not affect serum metabolites except for glucose, cholesterol and triglyceride.

11

Mitochondrial DNA mutations in human neoplasia

51%

Czarnecka A. M., Golik P., Bartnik E.

Journal of Applied Genetics

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2006

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tom 47

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nr 1

Many models of tumour formation have been put forth so far. In general they involve mutations in at least three elements within the cell: oncogenes, tumour suppressors and regulators of telomere replication. Recently numerous mutations in mitochondria have been found in many tumours, whereas they were absent in normal tissues from the same individual. The presence of mutations, of course, does not prove that they play a causative role in development of neoplastic lesions and progression; however, the key role played by mitochondria in both apoptosis and generation of DNA-damaging reactive oxygen species might indicate that the observed mutations contribute to tumour development. Recent experiments with nude mice have proven that mtDNA mutations are indeed responsible for tumour growth and exacerbated ROS production. This review describes mtDNA mutations in main types of human neoplasia.

Ograniczanie wyników

Polyamines as regulators of cell activation

Isolation and expression pattern of RGS21 gene, a novel RGS member

Optimizacija raboty dizelejj sel'skokhozjaistvennykh mashin i traktorov

Regulator of G-protein signalling expression and function in ovarian cancer cell lines

Conception of the small-pieces building production’ automation

The cystic fibrosis neutrophil: a specialized yet potentially defective cell

Optimisation of parameters of diesel’s regulator and loading

Structure of small G proteins and their regulators

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I-IGFBP expression pattern

Effects of different energy levels and ambient temperatures on serum thyroid hormones and some biochemical values in Holstein steers

Mitochondrial DNA mutations in human neoplasia