Wyniki wyszukiwania

1

Vascularization of zygotic and somatic embryos of Arabidopsis thaliana

100%

Fras A., Smolen B., Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica

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2008

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tom 50

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nr 2

43-48

Somatic embryos were induced from immature zygotic embryos of Arabidopsis thaliana cultured on Gamborg basal medium (B5) supplemented with dichlorophenoxyacetic acid (2,4-D) at 5 µM l-1. The somatic embryos were of unicellular origin. The sequence of divisions and the orientation of newly formed walls resembled the Onagrad pattern of early embryogenesis. Histological studies revealed no connection between the somatic embryos and explant tissue. In contrast to zygotic embryos, in late heart-shaped somatic embryos both sieve and tracheary elements were present. The sieve elements that formed in somatic embryos were characterized by larger plates than normal sieve elements observed in seedlings. Typical features of the tracheary elements in somatic embryos were irregular shape and thickening of the secondary walls.

2

Genetic differences in three regeneration systems of winter rye - selection of a universally responding line.

84%

Rakoczy-Trojanowska M., Smiech M., Malepszy S.

Journal of Applied Genetics

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1996

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tom 37A

3

Comparison of fluorescence in cucumber [Cucumis sativus L.] leaves of plants derived from in vitro regeneration system and from seeds

84%

Murkowski A., Burza W., Skorska E.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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2000

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tom 48

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nr 1

4

Gene insertion into strawberry leaf disk regeneration system using particle bombardment

67%

Sawahel W., Saker M.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 3

Strawberry has previously been transformed using Agrobacterium-mediated DNA transfer. In this paper, we present a process for delivering foreign genes into strawberry leaf disks using particle bombardment. Expression of foreign DNA into strawberry leaf disks (Fragaria X ananassa Duch.) was detected - using in situ GUS assay-following bombardment with tungsten particles coated with plasmid DNA (pBI221.23) that coded for the selectable (hygromycin phosphotransferase [hpt] and the screenable (β-glucuronidase [GUS]) marker genes. Both genes are under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. The criteria of stability of phenotypes after the removal of selective pressure, Southern blot hybridization and segregation analysis were used to confirm the mitotic stability of the foreign gene and its stable integration into the strawberry genome progeny. The relative simplicity of this system recommends its future use for the production of genetically modified strawberry.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Cellular and Molecular Biology Letters

1 Journal of Applied Genetics

1 Burza W.

1 Fras A.

1 Malepszy S.

1 Maluszynska J.

1 Murkowski A.

1 Rakoczy-Trojanowska M.

1 Saker M.

1 Sawahel W.

1 Skorska E.

1 Smiech M.

1 Smolen B.

1 2008

1 2000

1 1997

1 1996

Vascularization of zygotic and somatic embryos of Arabidopsis thaliana

Genetic differences in three regeneration systems of winter rye - selection of a universally responding line.

Comparison of fluorescence in cucumber [Cucumis sativus L.] leaves of plants derived from in vitro regeneration system and from seeds

Gene insertion into strawberry leaf disk regeneration system using particle bombardment