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Artificial aeration was added to an integrated vertical-flow constructed wetland (called aerated IVCW hereafter) for improving water purification performance. The results showed that both oxygen levels and aerobic zones were increased in the wetland substrates. The electric potential (Eh) profiling demonstrated that artificial aeration maintained the pattern of sequential oxic-anoxic-oxic redox zones within the aerated IVCW chambers in winter, while only two oxic-anoxic zones were present inside the non-aerated IVCW in the cold seasons. The nitrification/denitrification processes and organic matter decomposition were enhanced by artificial aeration since the removal efficiency of NH₄⁺-N and BOD₅ were significantly improved in all seasons, particularly in winter. It seemed that artificial aeration could compensate for the absence of plant-mediated oxygen supply, though the low temperatures and plant dieback still affected the removal efficiency of COD and TN in the winter. Eight hours of artificial aeration per day was sufficient to eliminate the significant accumulation of NO₃⁻-N previously observed in the effluent from continuously aerated subsurface-flow constructed wetlands. These results suggest that the aerated IVCW could treat domestic sewage more efficiently, especially in winter.
The changes of redox potential, pH, nitrate (V) concentration and N2O emission from high and low peat soils having been subjected to flooding were studied. Peat samples were taken from the Łęczyńsko-Włodawskie Lake District and incubated in a water-saturated state, under laboratory conditions, in the dark, at room temperature, for 55 days. Each week, the redox potential (Eh), and NO3- concentrations of the solutions collected and N2O content in gaseous phases were measured. The variations in the NO3- concentration in low peat soil were correlated with changes in the amount of nitrous oxide produced, indicating denitrification. The maximal N2O emission (15 mg N2O-N m-2d-1) from the low peat soil was measured at the beginning of the incubation.
Properties of peat reflect the peat-forming environment, peat development processes and the types of peat-forming plant. They also enable peat classification and quality evalution. Investigation of the physicochemical properties of organic matter is the key to understanding the history, evolution and geology of bogs. The purpose of this study was to determine some physicochemical properties, such as pH, Eh, electrolytic conductivity, carbon forms in water extracts and dry peat samples (TOC, IC, TC), degree of decomposition, macrofossil plant analysis and ash content, in a whole stratigraphic profile of bog sediment located near Lake Moszne. The research comprised a 17.5 ha lake, which was described in the 1990s as a dystrophic one, and the adjacent mires. The open water surface of the lake is surrounded by a 30–150 m wide belt of floating mat, composed of peatmosses and sedges with the dominant Sphagno-Caricetum rostratae association, all representing vegetation typical for transitional mires. These communities are most often found in the Polesie Lubelskie Region, in old lakes, and usually on their floating mat. The research material was collected from such a site. The analysis demonstrated acidic pH (4.17 in profile I and 4.08 in profile II, respectively) in the layer of 0-0.65 m, whilst from the 1.50 m depth to the mineral bottom the pH increased to nearly neutral (pH = 6.62 ± 0.18 in profile I and pH = 6.45 ± 0.12 in profile II). Redox potential in the surface layer corresponds to good oxygenation of mineral soils: 577 mV and 490 mV for profile I and II, respectively. A nearly linear decrease of Eh was observed to about 118 mV at the depth of 2.50 m. Lower than that, down to the depth of 3.50 m, the Eh value was stabilized. The graphical presentation of the Eh-pH relationship shows that in both cases (profiles I and II) aerobiosis prevails to the depth of 0.45-0.65 m, confirming that oxygen continues to be the final electron acceptor.
This paper proposes a new solution for improving oxygenation state of anaerobic medium by means of optical fibres. Visible light (400-750 nm) of varying intensity (811-4866 lx) was introduced through optical fibres to an anaerobic medium (Eutric Fluvisol) for 10 days, which could activate phototrophic microorganisms producing oxygen, and indirectly change the redox potential (Eh) and the gas composition formed during the incubation period. Control showed a significant decrease of Eh from the initial level of 320.8 mV to 50.6 mV at the end of incubation. Illumination caused buffering of Eh of tested medium. In these reactors ΔEh was 130.7 mV for 811 lx, 80.7 mV for 4866 lx and the most advantageous combination was 2433 lx where ΔEh was only 30.2 mV. In the illuminated units maximal concentration of oxygen was ~2.5% (811 lx), ~6% (2433 lx) and ~5.1% (4866 lx). The formation of N2 at about 20% for the combination of 2433 lx and 4866 lx, and about 15% for 811 lx was also observed. Respiration activity of phototrophs revealed a high level of CO2 1.3% (811 lx), while the stronger illuminations led to CO2 concentrations of only 0.5% which was connected with intense binding of this gas in the photosynthesis process. Obtained results emphasise the key role of light in anaerobic soil medium. Oxygen produced by the activity of phototrophs may indirectly affect the redox state by Eh buffering and thus prevent anaerobiosis. It also affects gas formation, which may have positive environmental consequences.
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PCBs in phytoplankton in the Odra Estuary

84%
Eleven PCB congeners were determined in phytoplankton samples collected from the Odra Estuary at 9 stations in 2001–2002. The PCB concentrations were related to the temperature, turbidity, salinity, oxygen and redox potential of the water as well as to the pigment content in the samples. The results indicate that phytoplankton and the detritus derived from it play a crucial role in the distribution of PCBs, their transfer from the water column to sediments and from the Estuary to the sea. The species composition of the phytoplankton occurring in this area could also be very important as regards the sorption of PCBs.
The paper presents research results concerning water retention curves, pore size distribution, and redox potential in profiles of muck-like soils under varied agricultural use. It was found that relatively favourable conditions for plant growth and development occur only in the surface horizons of muck-like soils, where the amount of water easily available for plants is at the level of 10-15%. Tillage of muck-like soils as arable fields for crop plants with low requirements, such as oat, potato, or field vegetable crops (cabbage) results in an almost two-fold decrease, in their muck-like horizons, of the content of large pores from 34 to 19% by vol., and an increase in the content of medium pores from 10 to 14-15% by vol., as compared to multi-year meadow cultivation. The studied soil profiles, with pore size distribution favourable for the processes of drying, are characterised by a strong resistance to reduction, i.e. redox potential values above +400 mV, observed within a broad range of soil water potential values, and which mean that one should not expect nitrate reduction in the soil profiles studied, and consequently no generation and emission of nitrous oxide (N₂O) to the atmosphere. The muck-like soil profiles studied can also be classified among soils with weak emission of carbon dioxide, which means that the soils do not contribute to the process of atmospheric/climatic warming.
Soil enzyme activity indicates potential of the soil to sustain biochemical processes, which are essential for the maintenance of soil fertility. Dehydrogenase activity reflects total oxidative activity of soil microflora, catalase activity is responsible for the decomposition of H2O2. Redox potential (Eh) is an index of the soil aeration status. Effects of soil temperature and water content on Eh, soil dehydrogenase activity and catalasc activity in an Eutric Histosol at three different fields planted with Papulus nigra, Salix amencana, and grasses (with Alopecurits pratensis, Phalaris arundinacea, Festuca pratensis is as dominating species) were studied at the depths of 10, 30, 50, 70 cm. Enzyme activity and redox potential showed seasonal fluctuations following natural changes of soil water content and temperature. Dehydrogenase activity was significantly correlated with soil temperature and water content (r=0.37** and r=0.27***, respectively). Redox potential showed a significant correlation with soil water content (r=-0.32*** ). Catalase activity did not exhibit any correlation with the tested parameters. The type of plantation influenced enzyme activities; tree cover was characterised by higher dehydrogenase and catalase activities than meadow soils.
Dehydrogenase and catalase activities were measured in a Eutric Histosol irrigated with municipal wastewaters (Lublin, Poland) purified by a two-step (mechanical and biological) treatment. Soil enzyme activity was used to test the biochemical status of the soil-plant system, the last step of wastewater purification. Three experimental fields, each of I ha area, were planted with Populus nigra, Salix americana, and grasses (with Alopecurus pratensis, Phalaris arundinacea, Festuca pratensis as dominating species). The fields were divided into three parts: not flooded control (A), flood-irrigated 10 times per year with 60-75 mm (B), and flood-irrigated 10 times per year with 120-150 mm (C) of wastewater per irrigation. The enzyme activity was measured several times during the first 2 years of wastewater application in soil sampled from control and flooded plots (0-10, 10-30, 30-50, 50-70 cm depth). Simultaneously, redox potential at the same depths was measured with permanently installed Pt electrodes. Irrigation with municipal wastewater elevated soil dehydrogenase activity on average by 44% and 27% for the low and high irrigation doses, respectively, but reduced catalase activity at the high irrigation dose on average by 12.4% (significant at P < 0.001). Plant cover significantly influenced soil dehydrogenase and catalase activities.
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