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Anthocyanin spot on the leaves of red clover is inherited independently of light spot and uniform leaf colour. The gene determining anthocyanin spot is probably localized on the other chromosome than the gene determining light spot on the leaves. A short flower tube is also determined by a single recessive gene of a pleiotropic action. It simultaneously causes reduction of pistil style, thickening of the endothecium layer in anthers - entails their indehiscence, pollen grain-agglutination and often deformation, no division of part of pollen grains into a vegetative and generative cells as well as germination of single grains in pollen sacks. The changes in the structure of endothecium and pollen grains cause almost complete male and female infertility of plants with short flower tubes.
The aim of this study was to determine the composition of nitrogen fractions in alfalfa and red clover, which differ in proteolytic activity, and to evaluate the effect of wilting on changes in nitrogen fractions in alfalfa and red clover herbage. Total nitrogen was divided into protein and non-protein nitrogen, and the amino acid profile of protein was analyzed. Buffer-soluble nitrogen (BSN), including buffer-soluble protein nitrogen (BSPN) and non-protein buffer-soluble nitrogen (NPBSN), was determined. The NPBSN fraction was further subdivided into peptide nitrogen, amino acid nitrogen, neutral detergent-insoluble nitrogen (NDIN) and acid detergent-insoluble nitrogen (ADIN). Wilting in the field to 40% dry matter content (the swath was tedded once) did not reduce the total nitrogen content of alfalfa and red clover herbage, although it affected the concentration of the BSNfraction, in particular NPBSN, and free amino acid nitrogen. During alfalfa wilting, the soluble protein content decreased and the concentrations of non-protein nitrogen compounds increased, mostly due to an increase in free amino acid nitrogen. A reverse trend was observed during red clover wilting – the concentrations of non-protein nitrogen compounds decreased and soluble protein content increased. A decrease was also noted in peptide nitrogen, NDIN and ADIN. Wilting of alfalfa and red clover had no adverse effect on the amino acid profile of protein. The concentrations of essential amino acids that limit milk protein synthesis, i.e. Lys, Met, His and Arg, did not decrease. Legume wilting in the field contributes to an increase in the concentrations of soluble nitrogen in the plant material intended for ensiling.
The aim of the study was to determine changes in nitrogen fractions during the ensiling of lucerne and red clover herbage in bales. Protein nitrogen and non-protein nitrogen compounds in herbage and silage were distinguished. Furthermore, buffer soluble nitrogen compounds (BSN) were determined and divided into buffer soluble protein and non-protein nitrogen (BSPN and NPBSN, respectively). In addition, peptide nitrogen and amino acid nitrogen were distinguished within the NPBSN fraction, while nitrogen compounds were divided into those of low solubility (NDIN) and completely insoluble (ADIN). The research findings confirmed the effect of a legume plant species on changes in the content of nitrogen fractions during the ensiling in round bales. The ensiling of lucerne herbage grossly decreased the protein nitrogen fraction while raising the total soluble fraction of nitrogen. Nitrogen in the form of soluble proteins, NDIN and ADIN was the least affected. The study demonstrated a different response of the two legume plant species to ensiling with respect to all fractions. A high proportion of protein nitrogen in round bale wilted red clover silage was accompanied by a large contribution of insoluble nitrogen fractions. A large share of the protein nitrogen fraction in red clover silage does not necessarily mean a higher nutritional value of the protein because it may contain a large amount of protein compounds that are non-hydrolysable by bacterial and intestinal enzymes.
W pracy przedstawiono właściwości mechaniczne nasion diploidalnych i tetraploidalnych odmian koniczyny czerwonej uprawianych w różnych gęstościach siewu i w trzech latach badań. W tym celu nasiona poddano testowi jednoosiowego ściskania do założonego odkształcenia, za pomocą uniwersalnej maszyny testującej INSTRON. określono siłę niszczącą nasiono, odpowiadającą granicy wytrzymałości biologicznej (Fmax) i energię (pracę) powodującą zniszczenie struktury nasiona (Emax) oraz obliczono parametr odkształcenia nasion będący ilorazem siły (DF) i odkształcenia względnego (DD). Przeprowadzona analiza statystyczna wykazała różnice pomiędzy badanymi cechami mechanicznymi nasion w zależności od odmiany, gęstości siewu i kolejnych lat badań. Wszystkie parametry mechaniczne nasion zależały od warunków klimatycznych w latach badań; uprawa w warunkach niesprzyjających (nadmiar opadów) powodowała wzrost wartości tych parametrów. Wytrzymałość nasion na ściskanie zależała istotnie od ploidalności odmian koniczyny czerwonej oraz od warunków uprawy. Stwierdzono, że Fmax, Emax, oraz DF/DD określają w podobnym stopniu właściwości mechaniczne pojedynczych nasion koniczyny czerwonej.
The cause of seed dormancy relief may be various external factors, however the most data suggest particular role of temperature, especially it is seasonally changing environmental cue. The impact of temperature on hydrotime model parameters of red clover seeds has not been studied up to date. The aim of the study was to determine the water relations of red clover seeds during germination after different constant or fluctuating temperature pretreatment in a dry and moist seedbed, on the basis of the hydrotime model. The highest germination was obtained as a result of temperatures in a moist seedbed thanks to a shift of the mean base water potential towards negative values. Alternating positive temperatures broke the dormancy of red clover seeds to the greatest extent. The use of the hydrotime model to characterise and predict relief of combinational dormancy may be a very effective approach, especially for cultivars, which contains a small percentage of hard seeds. Red clover seeds do not need extreme temperatures or large amplitudes of temperatures alternation to break dormancy in temperate climates. Our results acknowledged the advisability of sowing red clover in autumn because exposition to winter and early spring conditions allow seeds to reach a high vigour and successfully emerge in spring.
The purpose of the study has been to determine the yield, chemical composition and nutritive value of green forage from di- and tetraploid forms of red clover grown in different seed density regimes. The yield of green forage was analysed in the first year of full use, in 2003 and 2004, having cut the plants in the early inflorescence phase (1st cut). In green forage samples, the following were determined: basic chemical composition, concentration of water soluble carbohydrates (WSC), fibre fractions (NDF, ADF and ADL) and macronutrients (P, K, Mg, Ca, Na). The nutritive value of green forage was expressed according to the INRA 1988 system. The yield of green matter from the tetraploid cultivars Bona and Jubilatka was higher than from the diploid cultivars Krynia and Parada. The cultivar Bona distinguished itself by its highest average yield of green matter, dry matter, total protein and net energy. The dry matter content was the most variable green forage quality characteristic. In both years, green forage from the diploid cultivars contained more dry matter than the one made from the tetraploid varieties. At the same time, seed density did not have any significant influence on the differentiation of green forage yields. Green forage from the two diploid cultivars was characterised by a similar energy and protein value and a better fill unit value (better potential intake by ruminants). The concentrations of phorphorus, potassium and calcium in green forage from the diploid forms were slightly higher compared to the tetraploid forms, unlike the level of magnesium, which was slightly lower. Irrespectively of the polidy level, the concentration of macronutrients, except phosphorus, was higher in the first year of the study. Considering the nutritional requirements of animals, green forage from the analysed red clover cultivars was characterised by an inadequate level of magnesium, deficient amount of sodium and an improper Ca:P ratio. The results suggest that diploid forms of red clover can potentially generate a higher nutrititive value than tetraploid ones.
The contents of four isoflavones: daidzein, genistein, formononetin and biochanin A were analysed in breeding stocks of red clover with the purpose to find stocks with the highest and lowest levels of these compounds. The total content of the isoflavones in twenty nine stocks studied, varied within the range of 0.70 to 1.19% D.M.
During two years of the investigations some morphological properties as well as the chemical composition of the one cultivar of the meadow clover (Trifolium pretense) and white clover (Trifolium repens) were compared with that of their ecotypes. The meadow clover was charac­terized in its first regrowth in cutting maturity and the white clover in the second regrowth in its pasture maturity. In both cultivars examined properties were characterized with the statistically higher parameters than in their ecotypes. The greatest differences between cultivars and ecotypes did not refer to the chemical composition.
Changes in the amino acid profile of protein during ensiling depend on the proteolytic potential of plant species, the rate of the wilting and acidifying of ensiled herbage and, on the other hand, on the microbiological processes and fermentation of amino acids. The interaction of these factors determines the subsequent efficiency of nitrogen utilization in the rumen. The aim of this study has been to determine the influence of ensiling in round bales on changes in the amino acid content in lucerne and red clover silages. The amino acid composition was determined by analyzing the content of amino acids in herbages and silages. The lucerne silage protein was characterized by a higher contribution of all amino acids except Trp in comparison with red clover silage. Ensiling in bales of wilted lucerne and clover herbage lowered the content of Asp, His (P < 0.01), Ile, Lys (P < 0.05) in both plants. The ensiling process and plant species affected (P < 0.05) the total content of amino acids per 100 g of protein, where the range of changes in both raw materials was different. The loss of amino acids observed during the ensiling of wilted lucerne in round bales should be regarded as typical, whereas changes in the amino acid profile of red clover should be considered as atypical. A significantly higher ADIN content in red clover than in lucerne silage indicates that a rapid and significant increase of temperature occurred in experimental bales, which were characterized by a low degree of density, and this temperature rise strongly affected the availability of amino acids of the red clover protein also to the current microflora in the silage. Ensiling in round bales changed the amino acid profile of the lucerne and red clover protein. The ensiling process in this technology, however, severely deteriorated the quality of the red clover protein by lowering the content of all essential amino acids except Cys and Met.
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