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Ca2plus differently affects hydrophobic properties of guanylyl cyclase-activating proteins [GCAPs] and recoverin

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Gorczyca W. A., Kobialka M., Kuropatwa M., Kurowska E.

Acta Biochimica Polonica

2003

tom 50

nr 2

Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca2+ -binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca2+ . Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca2+ -dependent binding of recoverin to Phenyl-Se- pharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca2+ ]free (10 uM) than at low [Ca2+ ]freee(10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca2+ ]free in recoverin and at low [Ca2+ ]freee in GCAP2. Such different changes of hydrophobicity evoked by Ca2+ appear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Gorczyca W. A.

1 Kobialka M.

1 Kuropatwa M.

1 Kurowska E.

1 2003

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Ca2plus differently affects hydrophobic properties of guanylyl cyclase-activating proteins [GCAPs] and recoverin