Wyniki wyszukiwania

1

Clofibrate and di[2-ethylhexyl]phthalate increase ubiquinone contents without affecting cholesterol levels

100%

Aberg F., Appelkvist E. L.

Acta Biochimica Polonica

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1994

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tom 41

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nr 3

Induction studies were performed on liver, muscle, heart, brain and blood by feeding Sprague-Dawley rats a diet containing a peroxisome proliferator, clofibrate or di(2-ethylhexyl)phthalate. Ingestion of these drugs resulted in an increase in the amount of two different types of ubiquinone homologues UQ9 and UQ10 found in rat. Phthalate proved to be the more effective drug, leading to a highly increased amount of ubiquinone in the liver. Increases were also found in all the above-mentioned organs except the brain. The UQ9 levels were raised to 400, 200,120 and 120%, of the respective normal values. The antioxidant and hypolipidemic agent, probucol, was used as a control to evaluate whether the increased ubiquinone level constituted a response to the elevated hydrogen peroxide pressure, resulting from the induced increase in fatly acid 0-oxidation. In the presence of probucol, ubiquinone levels were decreased in all the above-mentioned organs except heart and brain. Probucol had limited effects on the amount of cholesterol and did not significantly alter the amount of dolichol. The two peroxisome proliferators differed in their effects on cholesterol, as well as on dolichol levels which was induced by phthalate but not by clofibrate. The possible mechanisms involved, and the importance of low toxicity drugs which could elevate ubiquinone levels in various tissues, arc discussed.

2

Identification and tissue distribution of a novel rat glucocorticoid receptor splice variant

63%

Ju H., Wang X., Liu Z., Liu P., Zhao Y., Xiong R., Zhou Y., Chen X.

Acta Biochimica Polonica

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2009

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tom 56

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nr 1

109-113

Glucocorticoid receptor (GR) is a steroid hormone receptor that has been shown to play important roles in diverse cellular and physiological processes. More and more evidence has revealed that the effects of glucocorticoids are mediated by the glucocorticoid receptor through genomic or nongenomic mechanisms. A growing number of glucocorticoid receptor splice variants have been identified in human tissues, but few are known in rat tissues. In this work, a novel rGR cDNA, called rGRβ, was cloned from Sprague Dawlay (SD) rat liver. Sequence analysis revealed that the rGRβ mRNA was 39 base pairs (bp) shorter than the rGR mRNA reported earlier. The deleted segment is located in exon 1 and encodes 13 repeated glutamine residues. Both the rGR and rGRβ mRNAs were quantitated by Northern blot hybridization using non-homologous glucocorticoid cDNA probes. Results showed that the rGR and rGRβ mRNAs were most abundant in the lung, the least abundant in the heart, and there were more rGR and rGRβ mRNAs in the kidney than in the liver. The identification of rGRβ may contribute to the understanding of the genomic or nongenomic effects of glucocorticoids.

3

High fructose model of oxidative stress and metabolic disturbances in rats. Part I. Antioxidant status of rats' tissues

63%

Zagrodzki P., Joniec A., Gawlik M., Gawlik M., Krosniak M., Folta M., Barton H., Pasko P., Chlopicka J., Zachwieja Z.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 3

The objective of the study was to assess the effects of a high-fructose diet as opposed to a starch diet (standard or enriched, respectively) on oxidative stress indices in rats fed for 9 weeks. In some groups of the animals, fructose overload diminished cellular glutathione peroxidase (GPX1) activity in the liver, while in others, the enrichment of diet with buckwheat enhanced heart GPX1 activity. No change in GPX1 activity was detected in the testes. Similarly, different diets did not affect significantly either thioredoxin reductase (TR) in the testes and kidneys, or superoxide dismutase (SOD) activity of red blood cells. All study groups exhibited decreased malondialdehyde content in plasma and ferric reducing ability of plasma, as compared with control groups. Taken together, these findings support the hypothesis that a diet high in fructose significantly changes antioxidant defence status in rats, but it can be counteracted by the addition of an appropriate nutrient.

4

Immunohistochemical testing of a new anti-EGF antibody

63%

Seiwerth S., Guglietta A., Pecur L., Grabarevic Z., Djacic S., Hutinec Z.

Folia Histochemica et Cytobiologica

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1996

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tom 34

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nr 1

5

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The effect of caerulein-induced acute pancreatitis on the levels of serotonin [5-HT] and 5-hydroxyindoleacetic acid [5-HIAA] in various rat tissues

51%

Celinski K., Kleinrok Z., Pokora J., Czechowska G., Skrzydlo-Radomanska B., Cichoz-Lach H.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 2

Ograniczanie wyników

Clofibrate and di[2-ethylhexyl]phthalate increase ubiquinone contents without affecting cholesterol levels

Identification and tissue distribution of a novel rat glucocorticoid receptor splice variant

High fructose model of oxidative stress and metabolic disturbances in rats. Part I. Antioxidant status of rats' tissues

Immunohistochemical testing of a new anti-EGF antibody

The effect of caerulein-induced acute pancreatitis on the levels of serotonin [5-HT] and 5-hydroxyindoleacetic acid [5-HIAA] in various rat tissues