Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

RAPD techniques in the studies on a lymnaeid subgenus Stagnicola

100%

Rybska E., Pacak A., Szweykowska-Kulinska Z., Lesicki A.

Folia Malacologica

|

1999

|

tom 07

|

nr 4

2

Scanning of Geotrichum candidum genome by RFLP-PCR of rDNA and RAPD

86%

Piegza M., Barszczewski W., Witkowska D., Stempniewicz R., Robak M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

|

2006

|

tom 09

|

nr 3

3

Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.

86%

Niklas A., Olszewska D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2021

|

tom 102

|

nr 2

4

Genomic profiling of F1 hybrids of durian (Durio zibethinus) revealed by RAPD-PCR

86%

Prihatini R., Ihsan F., Indriyani N. L. P.

Journal of Horticultural Research

|

2016

|

tom 24

|

nr 2

5

Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker

86%

Mullainathan L., Sridevi A., Umavathi S., Sanjai Gandhi E.

International Letters of Natural Sciences

|

2014

|

tom 06

The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.

6

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

72%

Ho V. T., Ngo Q. N.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2018

|

tom 99

|

nr 4

7

Genetic variation of Salmo trutta L. populations from the catchment areas of the Rega, Parseta and Wieprza rivers evaluated by RAPD and SSR markers

72%

Achrem M., Skuza L., Kirczuk L., Domagala J., Pilecka-Rapacz M., Czerniawski R.

Folia Biologica

|

2015

|

tom 63

|

nr 1

8

The pathogenicity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil

72%

Werner M., Irzykowska L.

Phytopathologia Polonica

|

2007

|

tom 46

9

Molecular detection and comparison of Gaeumannomyces graminis var. tritici isolates originating from wheat and rye

72%

Irzykowska L.

Journal of Plant Protection Research

|

2007

|

tom 47

|

nr 3

299-308

Gaeumannomyces graminis is an etiologic agent of take-all, economically important disease of cereals worldwide. A polymerase chain reaction with variety-specific primers was successfully used for detection of G. graminis var. tritici in plant tissue. Obtained results showed that this diagnostic method is a very sensitive and useful tool for detection of the pathogen even before disease symptoms arise. DNA polymorphism revealed by RAPD-PCR with three arbitrary primers was suitable for assessing genetic variation among Ggt isolates originating from wheat and rye.

10

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

72%

Karakulska J., Pobucewicz A., Nawrotek P., Muszynska M., Furowicz A. J., Czernomysy-Furowicz D.

Polish Journal of Veterinary Sciences

|

2011

|

tom 14

|

nr 2

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

11

Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers

72%

Dobrowolska A., Debska J., Kozlowska M., Staczek P.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 2

Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.

12

Characterization of Klebsiella pneumoniae strains isolated from urinary tract infection: Detection of ESBL characteristics, antibiotic susceptibility and RAPD genotyping

72%

Aladag M. O., Uysal A., Dundar N., Durak Y., Gunes E.

Polish Journal of Microbiology

|

2013

|

tom 62

|

nr 4

In this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K. pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K. pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%), the resistance rates of non ESBLproducing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.

13

Morphological and molecular differentiation of the Croatian populations of Quercus pubescens Willd. [Fagaceae]

72%

Franjic J., Liber Z., Skvorc Z., Idzojtic M., Sostaric R., Stancic Z.

Acta Societatis Botanicorum Poloniae

|

2006

|

tom 75

|

nr 2

123-130

Taxonomy of the genus Quercus L. is very complicated and often controversial because of its great variability and intense gene flow among the related species. The purpose of this research was to determine morphological and molecular variation, relationships and taxonomic status of the Croatian populations of Quercus pubescens Willd. using morphological analysis of the leaves and RAPD-PCR technique. The results of the morphological and molecular analyses were very similar, both showing differentiation of the southern (Mediterranean) from the northern (Continental) pubescent oak populations. These two groups were clearly separated and the estimated gene flow among the populations that belong to different groups (Nm=1.38) is significantly less than among the populations that belong to the same group (Nm=3.70). The obtained results were compared to the available studies. This study confirms a high variability of the Q. pubescens populations, but differences were not so big to confirm the opinion of existence of several species in this area. The conclusion is that the southern Croatian populations could be pure Q. pubescens populations, while the peculiarities of the northern Croatian populations originate probably from the Q. petraea introgression.

14

RAPD analysis of genetic structure in four natural populations of Taxus baccata from southern Poland

72%

Zarek M.

Acta Biologica Cracoviensia. Series Botanica

|

2009

|

tom 51

|

nr 2

67-75

This work assessed genetic diversity and genetic structure using random amplified polymorphic DNA (RAPD) variation in 120 individuals of four natural populations of Taxus baccata growing in southern Poland (3 in mountains and one in lowland) to obtain basic information on this natural resource. With 9 primers, 185 highly reproducible and clear RAPD bands were obtained. Genetic diversity within populations was relatively high, with percentages of polymorphic bands ranging from 48.65% to 77.30%, averaging 69.59% (Shannon index 0.311). Global AMOVA showed that genetic variation between populations accounted for 26% of total variation, with the remainder (74%) occurring within population. Pairwise ΦPT values were not correlated with geographic distance. Two groups of populations were distinguished by ANOVA and principal coordinate analysis (PCO) based on a Euclidean metric: those growing in mountains (Nowa Wieś, Cisowa Gora, Zadni Gaj), with higher internal diversity, and those growing in lowlands (Liswarta), with lower internal diversity. The results are typical for an outcrossing, wind-pollinated and long-lived woody species

15

Assessment of genetic diversity in cultivated tomato (Solanum lycopersicum L.) genotypes using RAPD primers

72%

Sharifova S., Mehdiyeva S., Theodorikas K.

Journal of Horticultural Research

|

2013

|

tom 21

|

nr 1

16

Ascochyta blight (Ascochyta syringae) of lilac (Syringa vulgaris L.)

58%

Kosiada T.

Acta Scientiarum Polonorum. Hortorum Cultus

|

2016

|

tom 15

|

nr 4

Lilac (Syringa vulgaris L.) is a popular ornamental woody plant grown for its very decorative flowers and large, dark-green leaves. The leaves remain on the shrubs for a long time. The fungus, Ascochyta syringae, is a pathogen which deteriorates the decorative value of the leaves. It causes brown irregular spots on leaves. In this study, 20 fungal isolates were tested in terms of their pathogenicity towards the leaves of S. vulgaris, and mycelium growth rate, while genetic variability was determined by RAPD-PCR. It was found that some isolates do not cause the formation of brown spots on leaves. Isolates differed considerably in terms of mycelium growth rate, ranging from 0.5 mm day⁻¹ (B96 at 30°C) to 8.8 mm day⁻¹ (B92a at 25°C). A positive dependence between mycelium growth and the capacity to cause leaf spots was observed. No close dependence was found between the genetic variability of isolates and the other examined traits of the isolates.

17

PCR methods in meat species identification as a tool for the verification of regional and traditional meat products

58%

Spychaj A., Mozdziak P. E., Pospiech E.

Acta Scientiarum Polonorum. Technologia Alimentaria

|

2009

|

tom 08

|

nr 2

5-20

In the times of industrial food production, regional and traditional food articles provide an attractive alternative for people looking for unforgettable sensory impressions. Regional or traditional food, commonly recognized as palatable and healthy, is also, for many consumers, a unique, sentimental journey back to tastes from childhood times. A gradual increase of demand for this type of food articles as well as relatively high prices of these products may generate among unscrupulous food manufacturers a number of improper production practices, e.g. replacement of a more expensive meat by a less expensive alternative. Species composition of meat products can be verified using chromatographic, immunological, electrophoretic, or genetic methods. One of the genetic methods applied in examining the authenticity of food composition, including meat and its products, is the polymerase chain reaction (PCR). This paper presents the most important techniques utilizing this technology to identify the origin of specific meat components constituting part of regional or traditional food articles. It was demonstrated that PCR techniques, in combination with species-specific primers, PCR-RFLP, PCR-SSCP and real-time PCR, allow identification of meat species occurring in-dependently or in mixtures with other meat species as well as meat subjected to thermal treatment or other technological processes in the course of industrial production. The only exception is the PCR-RAPD method that fails to identify meat species in the case of strong DNA degradation or in complex meat mixtures

Ograniczanie wyników

RAPD techniques in the studies on a lymnaeid subgenus Stagnicola

Scanning of Geotrichum candidum genome by RFLP-PCR of rDNA and RAPD

Application of the RAPD technique to identify genetic diversity in cultivated forms of Capsicum annuum L.

Genomic profiling of F1 hybrids of durian (Durio zibethinus) revealed by RAPD-PCR

Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

Genetic variation of Salmo trutta L. populations from the catchment areas of the Rega, Parseta and Wieprza rivers evaluated by RAPD and SSR markers

The pathogenicity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil

Molecular detection and comparison of Gaeumannomyces graminis var. tritici isolates originating from wheat and rye

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers

Characterization of Klebsiella pneumoniae strains isolated from urinary tract infection: Detection of ESBL characteristics, antibiotic susceptibility and RAPD genotyping

Morphological and molecular differentiation of the Croatian populations of Quercus pubescens Willd. [Fagaceae]

RAPD analysis of genetic structure in four natural populations of Taxus baccata from southern Poland

Assessment of genetic diversity in cultivated tomato (Solanum lycopersicum L.) genotypes using RAPD primers

Ascochyta blight (Ascochyta syringae) of lilac (Syringa vulgaris L.)

PCR methods in meat species identification as a tool for the verification of regional and traditional meat products