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This study was conducted during the rut in a protected valley (Vialais) inhabited by one permanent population of ewes. This valley formes part of a larger hunted area. Rams which segregate from ewes entered the Vialais during the pre-rut, first young rams while the oldest rams arrived later. These latter stay in the company of ewes only when most lambs are conceived. The removal of old males shifted the male population structure, favoring young males. Old males tended a low proportion of ewes in mating groups which was used as an index of the male mating success. A larger proportion of young males successfully tended estrous ewes than in protected populations of wild sheep. Despite that, the population is highly productive which contradicts the arguments that precocious involment of young males in reproduction may be detrimental to population productivity.
The influence of different extender osmolality levels and the presence of different cryoprotectants on the post- thawed semen's characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender according to two-step dilution procedures. The final concentrations of the cryoprotectants: 6% glycerol, 6% 1,2- propanediol, 62.5 mM sucrose, and 62.5 mM trehalose were studied in three different extender B osmolality levels (350, 375, and 400 mOsm). The osmolality affected significantly the post-thawed semen's motility, defected acrosomes (DA), total morphological defect (TMD), along with the sperm's plasma membrane integrity (HOST). Type of cryoprotectant exerted significant effect (P<0.001) on the post-thawed semen's motility, DA, TMD, and HOST. There was a significant interaction between the osmolality and cryoprotectant on the post-thawed motility, DA and TMD, but not on the HOST. In general, post-thawed motility, acrosomal, morphological, and membrane integrity of the semen frozen with semen extender at 400 mOsm were better than those of 350 and 375 mOsm, regardless of the type of cryoprotectant. Glycerol and 1,2- propanediol, compared to sucrose, trehalose, and control groups, did not protect the post-thawed acrosome and morphological integrity, though it did protect motility and HOST. It was concluded that glycerol based extenders with a high osmotic pressure (400 mOsm) was a better choice for ram semen freezing compared to sucrose, trehalose, and cryoprotectant free extenders. The detrimental effect of glycerol on DA and TMD could be overcome by combining glycerol with sugars and by increasing the osmotic pressure of the extender used for semen cryopreservation. Further research on the cryopreservation of ram semen should focus on the extender osmolality and combination of different cryoprotectants.
Fattening performance and carcass compositions were compared between Akkaraman (A), Kývýrcýk x Akkaraman F2 (KAF2 ), Kývýrcýk x (Kývýrcýk x Akkaraman) first backcross (KAB1 ), Chios x Akkaraman F2 (CAF2 ) and Chios x (Chios x Akkaraman) fist backcross (CAB1 ) ram lambs. After weaning at 3 months of age, the lambs were fed ad libitum concentrate and 300 g of alfalfa hay per lamb, per day. Six lambs of each genotype were slaughtered at 45 kg live weight and the carcasses were evaluated. The average daily weight gain and feed conversion efficiencies were 284, 271, 279, 282, 274 g and 4.92, 4.81, 4.76, 4.89, 5.01 for A, KAF2 , KAB1 , CAF2 and CAB1 , respectively. Corresponding values for cold dressing percentages were 49.28, 48.89, 48.18, 49.15 and 46.79, respectively. The weight of various fat depots differed (P<0.001 or P<0.01) among genotypes, but the weight of major cuts as a percentage of carcass weight did not differ significantly among genotypes except for percentage loin (P<0.05). It was concluded that there were no statistically significant differences for fattening performance among genotypes and that KA crossbred lambs had a higher percentage of loin and a lower percentage of bone in some of the major cuts. Also, there was a tendency for genotypes with lower tail fat weight to have a higher depot fat.
Ejaculates from three rams were collected with artificial vagina at one day (OD1) and four day intervals (FDsI). Part of the semen samples were pooled, diluted with skim milk based extender: -egg yolk (10%) -glycerol (5%), loaded into 0.25 ml straws, equilibrated, frozen, and afterwards stored in liquid nitrogen. The following spermatozoon parameters were determined in fresh and frozen-thawed semen: motility, viability, abnormality, abnormal acrosome, and membrane integrity. Semen collected at ODI had significantly higher freeze-ability compared with semen collected at FDsI for spermatozoon motility. Frozen-thawed semen collected at ODI had higher viability and membrane integrity, and lower abnormality and abnormal acrosome percentages compared with FDsI. It was concluded that in order to use cryopreservation of the Tushin ram semen, ODI instead of FDsI collection should be preferred.
The purpose of the paper was to assess the effect of administering a feed containing flax seeds on the fatty acid composition and cholesterol content in the meat of goat kids and ram lambs. The basic physicochemical properties of the meat were also determined. The analyses showed a significantly higher (p≤0.05) protein content in the goat kids (20.20 g) as compared with the ram lambs (19.50 g). The other chemical composition parameters were similar. The pH1 and pH2 results confirmed correct glycolytic metabolism. The obtained values were 6.39 and 6.27 for the goat kids and ram lambs, respectively. The pH2 value was 5.70 for both groups of animals. The analysed goat kid meat was characterised by better UFA:SFA and PUFA:MUFA ratios. The addition of flax seeds significantly differentiated the CLA (p≤0.01) and cholesterol (p≤0.05) content.
The aim of this study was to examine the influence of cholecystokinin (CCK)-octapeptide (OP) and its amphibian analogue cerulein infusions on duodenal myoelectric and motor activities, as well as to compare the effects of CCK peptides on duodenal bulb and duodenal motility in non-fasted conscious rams. Five rams underwent implantation of bipolar platinum electrodes to the duodenal bulb, and distal duodenum, as well as a strain gauge force transducer near the duodenal electrode. During continuous myoelectrical and motor recordings, 0.15 M NaCl or CCK peptides were administered intravenously. Infusions of CCK-OP at doses of 5 and 50 ng/kg/min and infusions of cerulein at doses of 0.5 and 1.5 ng/kg/min were applied for 60 min and started 15 min after the onset of the duodenal phase 2b of the migrating motor complex. The higher infusion dose of the CCK-OP in the duodenal bulb triggered the strong inhibitory response within few minutes following the start of infusion while in the duodenum its inhibitory effect was shorter and arrived within 40-50 min following the onset of the infusion. The higher dose of cerulein evoked a reaction similar to CCK-OP response in the duodenal bulb while in the duodenum the clear inhibitory response arrived about 20 min earlier than after CCK-OP. A lower infusion dose of CCK peptides evoked less pronounced effects. It is concluded that CCK-OP inhibits ovine duodenal motility in a dose-and region-specific manner. This effect seems to be physiological.
Serum levels of T₃, T₄, and TSH hormones, some elements, and glucose with regard to their involvement for growth retardation in yearling rams were investigated. A total of 70 yearling rams of the Akkararnan breed, 50 of which were classified with a retarded growth, and the remaining 20 were classified as normal, were included in the study. Serum total T₃, total T₄, and TSH were measured using chemiluminescent enzyme-labelled immunometric assay with immulite kits. Serum levels of Zn, Cu, and Co were measured using atomic absorption spectrophotometer. Serum glucose was measured using auto analyser. There were significant differences between the yearling rams, with and without retarded growth for serum levels of T₃, Zn, Cu, Co, and glucose. However, there no significant differences for T₄ and TSH between the yearling rams, with and without retarded growth. The results of the study suggest that retarded growth in yearling rams is associated with the lower levels of serum total T₃, Zn, Cu, Co, and glucose; however, there is no scientific evidence for the association of T₄, and TSH in the condition.
The dynamics of spatial distribution was followed in 18 radio-collared male mouflons Ovis ammon musimon (Pallas 1811) belonging to a population living in low mediterranean mountains. It was greatly modified with age and a marked inter­individual variability was observed in young males. The modification of spatial behav­iour appeared to be based on the process of progressive segregation of the sexes outside the rutting period. Spatial distribution by adult males seems to be more structured. However, ceHain males of at least 4 years of age were seen to be sedentary throughout the annua] cycle which suggests that the social segregation of the sexes does not necessarly involve spatial segregation. The hypothesis of neotenization in males of the genus Ovis is supported. The rut gTound is used with fidelity, being a site of particular spatial attachment, which could be related to the fact that it seems to bo the birth site. Nevertheless, with age, fréquentation of the birth site steadily become reduced to functional use related to rutting activities.
Levels of Cortisol in blood serum of 50- and 100-day-old rams of Kamieniecka sheep (K) and its crossbreds (F,) with Berrichon du Cher (KxBCH) and Blackhead (K x B) rams before pre-slaughter handling and after slaughter were determined. No relationship between the Cortisol levels and the genotype of the animals were found. However, age-dependent differences in the concentrations of the hormone (P ≤ 0.01) were disclosed. In the group of 50-day-old rams, the level of Cortisol was 48.69 nmol/1 whereas in older animals it was 61.53 nmol/1. After handling and slaughter a significant increase in the hormone levels from 39.51 to 70.72 nmol/1 was found, presumably reflecting a pronounced stress response of the animal organisms.
The present study was conducted to immunolocalize 3b-hydroxysteroid dehydrogenase (3β-HSD), an enzyme metabolizing pregnenolone to progesterone in the choroid plexus of the lateral ventricle in sheep, as well as to measure progesterone concentration in cerebrospinal fluid (CSF) and plasma using radioimmunoassay (RIA). Akkaraman breed rams (n = 16) and ewes (n = 16) were utilized in the study. 3β-HSD was immunolocalized in choroid epithelial cells of the choroid plexus with an apparent cytoplasmic immunoreactivity. Progesterone was detected in CSF with no significant differences between the ewes (0.76 ± 0.14 ng/mL) and rams (0.74 ± 0.13 ng/mL) (p > 0.05). However, the plasma progesterone concentration in the ewes (0.27 ± 0.04 ng/mL) was significantly higher than that of the rams (0.11 ± 0.02 ng/mL) (p < 0.001). Consequently, CSF in sheep contains progesterone in significant levels. As evidenced by 3β-HSD immunoreactivity, choroid epithelial cells may be a site of progesterone synthesis in sheep.
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