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1

Accumulation of raffinose family oligosaccharides and galactosyl cyclitols in developing Vicia seeds

100%

Lahuta L. B., Gorecki R. J., Gojlo E., Horbowicz M.

Polish Journal of Natural Sciences. Supplement

|

2003

|

tom 01

2

The accumulation of raffinose in vegetative tissues of legume seedlings in response to cold and desiccation stress

84%

Lahuta L. B., Goszczynska J., Gorecki R. J.

Acta Physiologiae Plantarum

|

2007

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tom 29

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nr 1 Suppl.

3

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Studies on soluble carbohydrates in yellow lupin [Lupinus luteus L.] seeds of different age

84%

Zalewski K., Lahuta L. B.

Acta Societatis Botanicorum Poloniae

|

2007

|

tom 76

|

nr 4

309-312

Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons. Stachyose dominated in axes, and verbascose - in cotyledons. Other detected galactosides were: galactinol, galactosyl pinitols and galactosyl chiro-inositols (fagopyritols), but their content was several-fold lower than that of RFOs (in both axes and cotyledons tissues). The concentration of soluble carbohydrates indicated, that sucrose to RFOs mass ratio, or other changes in sugars composition are not indicators of seed storage.

4

Alpha-D-galactosidase activity in stored yellow lupin [Lupinus luteus L.] seeds

84%

Lahuta L. B., Gorecki R. J., Michalczyk D., Piotrowicz-Cieslak A. I.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

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2000

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tom 03

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nr 1

The activity of alpha-D-galactosidase and contents of soluble saccharides were studied in Juno yellow lupin (Lupinus luteus L.) seeds stored. Seeds harvested at three stages of maturity (15, 25, 35 days after flowering - DAF) were stored at 21°C for two years (frozen immediately after harvest) or were dried after harvest to 8% of water content and stored at +20°C for 5 and 6 years. A high alpha-D-galactosidase activity in maturing and mature seeds could have been one of the causes of decreasing viability and vigour of the seeds stored. The hydrolysis of raffinose oligosaccharides decreased the ratio of these saccharides to sucrose. The decrease in the content of raffinose oligosaccharides was accompanied by an increase in galactosyl cyclitol contents.

5

Effect of exogenous abscisic acid on accumulation of raffinose family oligosaccharides and galactosyl cyclitols in tiny vetch seeds [Vicia hirsuta [L.] S.F.Gray]

84%

Lahuta L. B., Gorecki R. J., Gojlo E., Horbowicz M.

Acta Societatis Botanicorum Poloniae

|

2004

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tom 73

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nr 4

The role of the abscisic acid (ABA) in biosynthesis of raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds was investigated. The ABA was applied through incubation of seed at various stage of its development. The level of RFOs and Gal-C was determined in seed maturing on plant and in seed maturing in vitro. In early stages of V. hirsuta seed development, the ABA activated the biosynthesis of galactinol, although the level of arisen galactinol quickly declined. In the later stages of V. hirsuta seed development ABA had stimulatory effect of RFOs and Gal-C biosynthesis. Influence of ABA on biosynthesis of a-galactosides in Vicia hirsuta seed seems to be dependent on abscisic acid concentration. Low concentration of ABA had stimulatory effect on a-galactosides biosynthesis, but high concentration of ABA inhibited the process.

6

Analizy chromotograficzne cukrów obecnych w produktach przemysłu cukrowniczego. I. Wzorce cukrowe.

67%

Bermjdez O. T., Orozco R. R.

Gazeta Cukrownicza

|

1989

|

tom 97

|

nr 07

7

The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

67%

Orlikowska T., Zawadzka M., Kucharska D., Lahuta L. B.

Acta Physiologiae Plantarum

|

2010

|

tom 32

|

nr 2

We examined the effects of cooling applied for 4 to 20 weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4℃ in the dark for 4 months, followed by subculture on a fresh medium for 4 weeks. The cooling of the cultures caused an increase in the adventitious shoot number and a decrease in the starch content and an increase in the soluble sugar content (monosaccharides, raffinose and stachyose). The accumulation of stachyose in response to cold is a new observation, and it suggests that raffinose and stachyose play important role in the acclimation of dwarf apple rootstocks to low temperatures.

8

Wplyw procesow technologicznych na zawartosc oligosacharydow w nasionach roslin straczkowych

67%

Amarowicz R.

Bromatologia i Chemia Toksykologiczna

|

1999

|

tom 32

|

nr 1

9-13

9

Uwagi o hemimorfiźmie sacharozy

67%

Van Hook A.

Gazeta Cukrownicza

|

1989

|

tom 97

|

nr 06

10

Cyclitols in maturing grains of wheat, triticale and barley

67%

Lahuta L. B., Goszczynska J.

Acta Societatis Botanicorum Poloniae

|

2010

|

tom 79

|

nr 3

In the present study, the feeding of stem-flag leaf-ear explants of wheat, triticale and barley with d-chiro-inositol and d-pinitol was used for modification of the composition of soluble carbohydrates in grains without genetic transformation of plants. Maturing grains indicated ability to uptake exogenously applied cyclitols, not occurring naturally in cereal plants, and synthesized their a-d-galactosides. The pattern of changes in soluble carbohydrates during grain maturation and germination was not disturbed by the uptake and accumulation of cyclitols. Both, d-chiro-inositol and d-pinitol as well as their a-d-galactosides can be an additional pool of soluble carbohydrates accumulated by maturing grains, without decreasing seeds viability. This is the first report indicating the possibility of introduction of cyclitols with potentially human health benefits properties into cereal grains.

11

Utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides

67%

Villaluenga C. M., Wardenska M., Pilarski R., Bednarczyk M., Gulewicz K.

Folia Biologica

|

2004

|

tom 52

|

nr 3-4

12

The effects of ABA on the accumulation of raffinose family oligosaccharides in maturing yellow lupin [Lupinus luteus L.] seeds

67%

Piotrowicz-Cieslak A. I., Tuchewicz K.

Polish Journal of Natural Sciences. Supplement

|

2003

|

tom 01

13

Loss of tolerance to desiccation in germinated Norway maple (Acer platanoides L.) seeds. Changes in carbohydrate content

67%

Pukacka S.

Dendrobiology

|

2001

|

tom 46

Carbohydrates were analyzed in Norway maple embryo axes and cotyledons after imbibition, in the middle of cold stratification, before germination and during radicle protrusion to 8-10 mm and 20-25 mm. Simultaneously desiccation tolerance of seeds was determined by tetrazolium (TTC) test, after desiccation of seeds to 10-20% of water content. The cotyledons were tolerant to desiccation throughout all stratification and germination period. Embryo axes became sensitive to desiccation when hypocotyls-radicle protrusion reached 20-25 mm length. In this period the significant increase of monosaccharides: glucose, fructose and galactose in embryo axes occurred. This was not observed in cotyledons. During the germination period significant decrease of sucrose and raffinose content was noted in embryo axes and cotyledons. Relatively less changes appeared in stachyose content in embryo axes while in cotyledons it decreased evidently. The mass ratio of sucrose to oligosaccharides was higher in cotyledons of germinated seeds. The marked decrease of mass ratio of oligo to monosaccharides was observed in embryo axes in the last period of germination. The role of carbohydrates in loosing tolerance to desiccation in germinated Norway maple seeds is discussed.

14

Desiccation induces accumulation of raffinose and stachyose in legume seedlings

67%

Gorecki R. J., Lahuta L. B., Goszczynska J.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

15

Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of alpha-D-galactosides in developing tiny vetch [Vicia hirsuta [L.] S.F.Gray] seeds

67%

Lahuta L. B., Horbowicz M., Gojlo E., Goszczynska J., Gorecki R. J.

Acta Societatis Botanicorum Poloniae

|

2005

|

tom 74

|

nr 4

In the present study we have investigated the effect of exogenous cyclitols on the accumulation of their galactosides and raffinose family oligosaccharides (RFOs), as well as on some enzymes important for their biosynthesis in seeds of tiny vetch (Vicia hirsuta [L.] S.F. Gray). Immature seeds during 6-day incubation with D-chiro-inositol (naturally does not appear in seeds of tiny vetch) were accumulated cyclitol and its galactosides (fagopyritols: B1 and B2). Short 4-hour incubation with D-chiro-inositol, and subsequent slow desiccation process caused accumulation of free cyclitol only, without biosynthesis of its galactosides. Feeding D-chiro-inositol to pods of tiny vetch induced accumulation of high levels of its galactosides (fagopyritol B1, B2 and B3) in maturing seeds. Similarly, feeding D-pinitol increased accumulation of its mono-, di- and tri-galactosides: GPA, GPB, DGPA and TGPA in tiny vetch seed. Accumulation of both cyclitols and their galactosides drastically reduced accumulation of verbascose. Inhibition of RFOs biosynthesis by elevated levels of free cyclitols suggests some competition between formation of both types of galactosides and similarity of both biosynthetic routes in tiny vetch seeds. Galactinol synthase (GolS) from tiny vetch seeds demonstrated ability to utilize D-chiro-inositol as galactosyl acceptor, instead of myo-inositol. Presence of both cyclitols, as substrates for GolS, caused synthesis of their galactosides: fagopyritol B1 and galactinol. However, formation of galactinol was more efficient than fagopyritol B1. D-chiro-Inositol and D-pinitol at concentrations several-fold higher than myo-inositol had inhibitory effect on GolS. Thus, we suggest that a level of free cyclitols can have an influence on the rate of galactinol biosynthesis and further accumulation of RFOs and galactosyl cyclitols in tiny vetch seeds.

16

Carbohydrates in Colobanthus quitensis and Deschampsia antarctica

67%

Piotrowicz-Cieslak A. I., Gielwanowska I., Bochenek A., Loro P., Gorecki R. J.

Acta Societatis Botanicorum Poloniae

|

2005

|

tom 74

|

nr 3

Eight to nineteen ethanol-soluble carbohydrate components were identified in vegetative tissues of Colobanthus quitensis and Deschampsia antarctica. The analysed carbohydrates included: monosaccharides, cyclitols, galactosyl cyclitols, raffinose family oligosaccharides, lichnose family oligosaccharides, kestose family oligosaccharides. The analysed vegetative tissues accumulated from 447 to 139 mg/g d.m. soluble carbohydrates in Colobanthus quitensis, Deschampsia antarctica respectively. The raffinose family oligosaccharides constituted 53.3% in Colobanthus quitensis of the identified soluble carbohydrate component pool. Vegetative tissues accumulated starch in Colobanthus quitensis 20.6 mg/g d.m. and 261.6 mg/g d.m. in Deschampsia antarctica. Anatomical and ultrastructural observations of vegetative part of Colobanthus quitensis and Deschmpsia antarctica revealed the presence of various ergastic materials in intercellular spaces, cell walls and protoplasts. Various parts of these plants contain insoluble, PAS positive polysaccharides in intercellular spaces and in cell walls. Chloroplasts of analysed tissues contained starch. Less starch was visible in young, growing parts of shoots of Colobanthus quitensis and Deschmpsia antarctica, more starch appears in mature, differentiated parts.

17

Galactooligosaccharides of seeds during growth of Phaseolus coccineus L. and Phaseolus vulgaris L.beans

59%

Kosson R.

Acta Agrobotanica

|

1995

|

tom 48

|

nr 1

75-81

The quantitative changes of raffinose and stachyose in seeds of Phaseolus coccineus L. and Phaseolus vulgaris L. during their growth and maturation in a two year experiment were investigated. Trace amounts of raffinose were found in Ph. vulgaris seeds during their vegetative growth in 1990. Time of raffinose accumulation in seeds in 1991 began not earlier than on 33rd day since inflorescence. Stachyose started to accumulate in seeds between 33rd and 47th day after inflorescence of all tested cultivars. It was noticed that stachyose and raffinose contents in seeds of most tested cultivars after ten years of storage did not differ significantly when compared to not stored ones.

18

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides

51%

Lorenc-Plucinska G., Stobrawa K.

Acta Societatis Botanicorum Poloniae

|

2005

|

tom 74

|

nr 1

Concentrations of total nonstructural carbohydrates (TNC), soluble carbohydrates, starch, sucrose, glucose, fructose, raffinose, galactose, stachyose, mannitol and specific activities of soluble acid (AI) and neutral (NI) invertases, sucrose synthase (SuSy), hexokinase (HK), fructokinase (FK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glucose 6-phosphate dehydrogenase (G6PDH) were analyzed in fine roots of Populus deltoides Bartr. ex Marsh growing at a polluted site (near copper smelters) and a control site (free from heavy pollution). Also chemical properties of the soil from both sites were assessed. In comparison with the control, fine roots from the polluted site contained greater concentrations of TNC, soluble sugars, starch and sucrose but less hexoses, so they had higher values of sucrolysis index (sucrose/hexoses). The activity of AI, NI and SuSy declined insignificantly, while specific activities of HK, FK, GAPDH and G6PDH were significantly inhibited. The results suggest that a long-term heavy metal stress leads to an accumulation of carbohydrates and altering activities of glycolysis and the oxidative pentose phosphate pathway in fine roots.

19

Wystepowanie kwasow aromatycznych i cukrow w kwiatach Centaurea cyanus L

43%

Swiatek L., Zadernowski R.

Acta Academiae Agriculturae ac Technicae Olstenensis. Technologia Alimentorum

|

1993

|

tom 25

231-239

Ograniczanie wyników

Accumulation of raffinose family oligosaccharides and galactosyl cyclitols in developing Vicia seeds

The accumulation of raffinose in vegetative tissues of legume seedlings in response to cold and desiccation stress

Studies on soluble carbohydrates in yellow lupin [Lupinus luteus L.] seeds of different age

Alpha-D-galactosidase activity in stored yellow lupin [Lupinus luteus L.] seeds

Effect of exogenous abscisic acid on accumulation of raffinose family oligosaccharides and galactosyl cyclitols in tiny vetch seeds [Vicia hirsuta [L.] S.F.Gray]

Analizy chromotograficzne cukrów obecnych w produktach przemysłu cukrowniczego. I. Wzorce cukrowe.

The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

Wplyw procesow technologicznych na zawartosc oligosacharydow w nasionach roslin straczkowych

Uwagi o hemimorfiźmie sacharozy

Cyclitols in maturing grains of wheat, triticale and barley

Utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides

The effects of ABA on the accumulation of raffinose family oligosaccharides in maturing yellow lupin [Lupinus luteus L.] seeds

Loss of tolerance to desiccation in germinated Norway maple (Acer platanoides L.) seeds. Changes in carbohydrate content

Desiccation induces accumulation of raffinose and stachyose in legume seedlings

Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of alpha-D-galactosides in developing tiny vetch [Vicia hirsuta [L.] S.F.Gray] seeds

Carbohydrates in Colobanthus quitensis and Deschampsia antarctica

Galactooligosaccharides of seeds during growth of Phaseolus coccineus L. and Phaseolus vulgaris L.beans

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides

Wystepowanie kwasow aromatycznych i cukrow w kwiatach Centaurea cyanus L