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1
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Changes in innate and adaptive immune response to Lagovirus europaeus GI.1 (Rabbit Haemorrhagic Disease Virus -RHDV) infection

100%
Hukowska-Szematowicz B.
Acta Biologica
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2020
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tom 27
2

Non-specific cellular immunity in rabbits experimentally infected with four Czech strains of the rabbit haemorrhagic disease virus with different pathogenicity

100%
Hukowska-Szematowicz B., Deptula W.
Polish Journal of Environmental Studies
|
2012
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tom 21
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nr 4
The rabbit haemorrhagic disease (RHD) virus was first described in 1984 in China, where it caused a rabbit plague characterized by an acute course. At present, the disease has spread to rabbits on all continents, and is characterized by morality reaching 100%. Research on the immune response in rabbits after infection by RHD virus strains has so far only been performed by the Deptuła team. In turn, it must be stated that similar research worldwide has been performed in the Chinese centre, yet referring exclusively to rabbits after immunization with inactivated RHD virus. Such research indicates that shortly after immunization, the immunity is coordinated by macrophages and lymphocytes T and B, while farther on the protection against the infection is conditioned by humoral immunity. Deptula's team has investigated 22 strains of RHDV in the aspect of non-specific cellular and humoral immunity, as well as specific cellular and humoral immunity including 3 French strains (FR-1, Fr-2, 9905RHDVa), 10 Polish strains (K-l, Kr-1, KGM, SGM, MAŁ, BLA, PD, GSK, Ż, ŻD), 4 German strains (Hagenow, Frankfurt, Triptis, Hartmannsdorf), 3 Italian strains (BS89-reference strain, Vt97, PV97), 1 English strain (Rainham), and 1 Spanish strain (Asturias). The strains were analyzed in the aspect of such parameters as capacity of adherence and absorption of PMN cells, PMN cell cidal property measured with spontaneous, stimulated, and spectrophotometric NBT test, stimulation index and PMN metabolic activity coefficient; and MPO activity, as well as concentration and activity of LZM. Also, the number was marked of lymphocytes T CD5+, Th with receptor CD4+, Tc/Ts with receptor CD8+, and the number of lymphocytes with receptor CD25+, as well as the percentage of lymphocytes B (IgM). The research indicates the presence of immunogroups within the RHD virus. Assessment of pathogenicity of the RHD virus is actually performed based on the mortality rate in rabbits infected with the virus, which is dictated by the fact that the virus has so far not been obtained in vitro. Niedźwiedzka et al. divided the 10 analyzed strains into strains with high pathogenicity with mortality of 90-100%, up to 36/48 hour of the study (BS89, Hagenow, Rainham, Frankfart, Asturias, Triptis, Hartmannsdorf, Pv97, 9905RHDVa), and strains with lower pathogenicity with mortality of 30% up to 36/48h (Vt97). In turn, Tokarz-Deptuła divided the 10 analyzed strains of the RHDV (including 8 Polish and 2 French) into strains with mortality of 80-100% (Fr-2, ŻD, GSK, SGM, Fr-1, Kr-1, MAL), strains with mortality of 60-65% (KGM, BLA), and strains with mortality below 60% (PD). The aim of our study was to record changes to parameters of non-specific cellular immunity (capacity of adherence and absorption of PMN cells, cidal capacity of PMN cells measured with spontaneous NBT test, stimulated and spectrophotometric, and stimulation index and metabolic activity ratio of PMN cells) in rabbits experimentally infected with 4 haemagglutinogenic Czech strains of the RHD virus: CAMPV-351 (reference strain), CAMP-561, CAMPV-562, and CAMPV-558, with different pathogenicity; which strains have not yet been analyzed in this respect. The assessment of pathogenicity of the analyzed strains of the RHDV was performed on the basis of mortality rate among rabbits infected with these strains. On the basis of the number and duration of changes to analyzed parameters of non-specific cellular immunity, the 4 analyzed Czech strains are determined to differentiate in the aspect of immunogenicity into three groups. The first group is formed by the most immunogenic reference strain CAMPV-351, the second - by two medium-immunogenic strains - CAMPV-561 and CAMPV-558, whereas the third one - by the least immunogenic strain CAMPV- 562. The results obtained in the area of pathogenicity are not reflected in the division of the analyzed Czech strains according to their immunogenicity.
3
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Phagocytosis of neutrophils in rabbits infected with antigenic variants of RHD (rabbit haemorrhagic disease) virus

100%
Niedzwiedzka-Rystwej P., Deptula W.
Polish Journal of Veterinary Sciences
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2012
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tom 15
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nr 1
The present study was aimed at determining changes in chosen elements of phagocytosis in rabbits infected with 3 antigenic variants of RHD – Hartmannsdorf, Pv97 and 9905, which differed in haemagglutination ability. The animals were tested for phagocytosis parameters, and the results revealed that the examined strains showed the differences. These variations regarded mainly Pv97 strain, as the intensity of the changes were 5 times stronger in comparison to strain Hartmannsdorf and 9905. As all of the strains examined are signified as antigenic variants, we have stated that this feature does not determine their immunological picture. The results suggest the existence of immunological dissimilarities among strains of the RHD virus, which was revealed for the first time in antigenic variants.
4
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Restriction enzyme analysis of the capsid protein gene of different strains of European brown hare syndrome virus

100%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
|
2001
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tom 45
|
nr 2
Eight Polish strains of European brown hare syndrome virus, isolated in 1992-93, 1998 and 2001, as well as 2 standard strains: French (F/st) and Italian (It/st) were analysed. The fragments of genome, including the capsid protein gene, were amplified by RT-PCR or N-PCR and digested by endonucleases: Xho I, Sst I, BamH I, Sma I, Hpa II and BsuR I. The results of restriction enzyme digestion were compared with restriction maps, obtained after computer analysis of nucleotide sequences of other European strains, available in GenBank. The results of analysis of Polish strains, isolated in different years, revealed the changes in restriction profiles between them and other European strains. The 3 out of 5 Polish strains detected in 1992-93 demonstrated the same restriction profiles, similar to French "GD" strain. The two other strains revealed, for same endonucleases, restriction patterns similar to ltalian "BS89" or German strains. The strains isolated in 1998 and 2001 demonstrated higher differences in restriction profiles in comparison with Polish and other European strains previously detected. These changes in nucleotide scquences of the part of genome studied, indicate the diversity of EBHSV.
5

Genetic variability of czech and German RHD virus strains

84%
Hukowska-Szematowicz B., Pawlikowska M., Deptula W.
Polish Journal of Microbiology
|
2009
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tom 58
|
nr 3
237-245
RHD (rabbit haemorrhagic disease) virus (RHDV) is the aetiological factor of the haemorrhagic disease of rabbits and is currently present on all continents. RHDV is a small, envelope-free virus containing genetic material in the form of a 7437-nucleotide long RNA strand. Studies indicate that genetic variability of RDHV strains originating from various parts of the world is approximately 14%, regardless the time and place of isolation. The aim of this study was to evaluate the genetic variability of 6 RHD virus strains from the Czech Republic (CAMPV-561, CAMPV-562, CAMPV-558) and Germany (Frankfurt, Wika, Rossi) based on analysis of fragment of a gene coding a nonstructural p30 protein. The largest variability of nucleotide sequences within the studied fragment was found for the Rossi strain and CAMPV-562 (13.5%) and CAMPV-558 (13.5%), Wika and Frankfurt (12.1%), and CAMPV-561 and Wika (11.2%). Among the Czech strains the largest genetic distance was noted for strains CAMPV-558 and Iowa (0.130/0.140), and in the case of the German strains, for Frankfurt and Iowa (0.123/0.132). A homology tree constructed based on a fragment of ap30 protein-coding gene divided the 14 analysed strains into IV groups of 88% homology. Phylogenetic relationships also divided the tested strains into 4 genetic groups (G1-G4). The larger genetic distance exists between the Czech and German strains and the American ones, and the smallest between them and the European strains.
6

Phylogenetic analysis of selected strains of rabbit haemorrhagic disease virus on the basis of N-terminal fragment of the gene encoding structural protein VP60

84%
Pawlikowska M., Hukowska-Szematowicz B., Deptula W.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 2
129-133
The purpose of the study was the phylogenetic analysis of 16 European strains of the rabbit haemorrhagic disease (RHD) virus identified in 1989-2004, on the basis of N-terminal fragment of the gene encoding structural protein VP60. The obtained sequences of the strains were compared to the sequences of 30 strains of the virus received from the GenBank gene database. As a result of phylogenetic analysis of all the strains, they were divided into six genogroups, which were principally formed on the basis of the time of their identification, and the place of isolation of the RHD virus, which confirms the present hypothesis of genogroups creation among this virus. Furthermore, in our studies, a clear and visible difference was obtained between the classic strains in genogroups 1-5, and the strains referred to as antigen variants - genogroup 6.
7

Non-specific humoral immunity in rabbits experimentally infected with nine Polish strains of the RHD (rabbit haemorrhagic disease) virus

84%
Tokarz-Deptula B., Adamiak M., Pastuszak-Gabinowska M., Deptula W.
Advances in Agricultural Sciences
|
2010
|
tom 13
|
nr 1
8

Comparative analysis of the gene encoding RNA-dependent RNA polymerase in RHD virus strains originating from various continents

84%
Hukowska-Szematowicz B., Lejkowska R., Deptula W.
Advances in Agricultural Sciences
|
2010
|
tom 13
|
nr 1
9
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Cytometric analysis of lymphocytes T and B in rabbits infected with non-haemagglutinogenic strains of RHD virus [rabbit heamorrhagic disease] - Rainham, Frankfurt and Asturias

84%
Niedzwiedzka-Rystwej P., Deptula W.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
157-162
The present paper refers to the cytometric analysis of lymphocytes T (with receptor CD5+), Th (with receptor CD4+), Tc/Ts (with receptor CD8+), lymphocytes CD25+ and lymphocytes B with receptor CD19+ in rabbits experimentally infected with strains of RHD virus - Rainham, Frankfurt and Asturias, not having haemagglutinogenic capacities, which makes them unique, as haemagglutinogenic capacity is a classic and typical property of most strains of this virus. The study was performed in the dynamic system, drawing blood samples from animals at hour 0, namely before the administration of the viral antigen, and then at 4, 8, 12, 24 and 36 h after the infection. The study indicated that Rainham and Asturias strains of RHD virus cause a similar amount of changes as the most immunogenic haemagglutinogenic strains CAMP V-561 and CAMP V-562 of the RHD virus do. In contrast, the Frankfurt strain of the RHD virus is characterised with 5-6-fold lower reactivity in this respect and is most similar to the least immunogenic haemagglutinogenic strain CAMP V-558 of the RHD virus.
10

Phylogenetic analysis of partial capsid protein gene of rabbit haemorrhagic disease virus [RHDV] strains isolated between 1993 and 2005 in Poland

84%
Chrobocinska M., Mizak B.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
The 18 RHDV strains, isolated between 1993 and 2005 in Poland, as well as one standard strain were used in these studies. The RT-PCR technique was carried out in order to amplify the 538 bp fragment located in the variable region of gene encoding capsid protein (VP60). The phylogenetic analysis of nucleotide sequences of 18 Polish and 25 foreign strains revealed that Polish strains were clustered to 4 genogroups. One of them from 1993 was grouped together with old European strains isolated between 1987 and 1990. Thirteen strains isolated between 1994 and 2004, as well as two strains isolated in 1994 and 1996, were clustered to two separate genetic groups. Two strains detected in 2004 and 2005, were located in genogroup of antigenic variants. The maximum nucleotide divergence among Polish strains (10.3%) was found between strain isolated in 1996 and antigenic variants. The alignment of deduced amino acid sequences of the VP60 confirmed the localisation of 2 Polish strains in genetic group of antigenic variants. The identical amino acid substitutions in the same positions, like in other antigenic variants, were observed. The relation between antigenic properties and genetic variations of strains was taken under consideration.
11

Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus

84%
Gromadzka B., Szewczyk B., Konopa G., Fitzner A., Kesy A.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr 2
Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5'-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.
12

Phylogenetic analysis of French strains of the RHD virus on the basis of a fragment of gene encoding C-terminal part of structural capsid VP60 protein

67%
Hukowska-Szematowicz B., Deptula W.
Advances in Agricultural Sciences
|
2010
|
tom 13
|
nr 1
13

Ingestion capacity and hematological parameters in rabbits experimentally infected with strains of RHD (rabbit haemorrhagic disease) virus differing in biological features

67%
Niedzwiedzka P., Tokarz-Deptula B., Deptula W.
Advances in Agricultural Sciences
|
2008
|
tom 12
14

Virusological image in the direct immunofluorescence test in rabbits experimentally infected with the RHD (rabbit haemorrhagic disease) virus

67%
Adamiak M., Miller T., Deptula T., Pastuszak-Gabinowska M., Tokarz-Deptula B.
Advances in Agricultural Sciences
|
2010
|
tom 13
|
nr 1
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