Wyniki wyszukiwania

1

Prevalence and genotypes of Enterocytozoon bieneusi in sika deer in Jilin province, Northeastern China

100%

Zhang X.-X., Cong W., Liu G.-H., Ni X.-T., Ma J.-G., Zheng W.-B., Zhao Q., Zhu X.-Q.

Acta Parasitologica

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2016

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tom 61

|

nr 2

2

Number and size of nucleoli in the spermatocytes of chicken and Japanese quail

100%

Andraszek K., Gryzinska M., Knaga S., Wojcik E., Smalec E.

Folia Biologica

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2012

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tom 60

|

nr 3-4

3

Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification

100%

Liu T., Xu J., Luo B., Chen J., Li Z., Li T., Pan G., Li X., Zhou Z.

Acta Parasitologica

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2015

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tom 60

|

nr 2

4

Organization of the 18S, 5S, 4S rRNA genes and the tRNA-like repeat in the mitochondrial genomes of three lupin species

100%

Karpinska B., Lesniewicz K., Pietkiewicz G., Augustyniak H.

Acta Biochimica Polonica

|

1994

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tom 41

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nr 4

Southern blots of mitochondrial (mt) DNAs of three Lupinus species cleaved with three restriction enzymes were probed with Lupinus luteus mtDNA fragments containing 18S, 5S rRNA genes or a tRNA-like repeat. Comparison of the number of hybridizing bands and their intensity suggested that the mt 18S and 5S rRNA genes occur mostly in one copy in the genomes of three lupin species. The exception concerned the Lupinus angustifolius 5S rRNA gene showing two hybridizing bands of unequal intensity. The results of hybridization of the lupin mitochondrial genomes with a probe specific for the Lupinus luteus tRNA-like repeat pointed to the presence of such a repeat in other parts of the genomes besides the vicinity of the 18S rRNA gene. Northern hybridization analysis showed the presence of 18S, 5S and tRNA-like repeat transcripts similar in size in all lupin species.

5

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Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction

100%

Golab E., Sobolewska A., Dzbenski T., Bitkowska E.

Wiadomości Parazytologiczne

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1998

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tom 44

|

nr 3

6

Prevalence of cdt-III gene encoding cytolethal distending toxin [CDT] among Escherichia coli strains isolated from pigs with diarrhea

100%

Osek J., Weiner M.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

|

nr 1

7

Comparison of the nuclear organiser region activity in four taxa of the family Canidae

100%

Pienkowska A., Zagalska M.

Journal of Applied Genetics

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2001

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tom 42

|

nr 4

8

Phylogenetic positions of two ciliates, Paranophrys magna and Mesanophrys carcini [Ciliophora: Oligohymenophorea], within the subclass Scuticociliatia inferred from complete small subunit rRNA gene sequences

100%

Shang H., Song W., Warren A.

Acta Protozoologica

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2003

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tom 42

|

nr 3

9

Application of multiplex PCR in routine microbiological diagnostics of Listeria monocytogenes and Listeria sp. strains in a meat-processing plant

100%

Medrala D., Dabrowski W., Szymanska L.

Polish Journal of Food and Nutrition Sciences

|

2003

|

tom 12/53

|

nr 1

A multiplex PCR assay designed by Bansal [1996] was applied in a routine screening test of a meat-processing environment aimed at detection of Listeria monocytogenes and species of the genus Listeria. Listeria spp. strains yielded a single 938-bp product indicating presence of 16S rRNA conservative sequence typical of the genus, whereas L. monocytogenes strains yielded not only the 938-bp product but also a 750-bp product - a result of amplification within region of the listeriolysin (hly A) gene. The assay was used to verify identification of 50 colonies performed using classical tests, including catalase and hemolytic activity, motility and API®LISTERIA (bioMérieux) biochemical tests. Among isolates, 4 strains (8%) were found characterised by contradictory results of biochemical and genetic tests. The possibility of simultaneous identification and differentiation of potentially pathogenic L. monocytogenes strains from other Listeria spp. during one reaction performed was proved.

10

Use of molecular and conventional techniques to identify and analyze genetic variability of Rhizoctonia spp. isolates

80%

Irzykowska L., Zoltanska E., Bocianowski J.

Acta Agrobotanica

|

2005

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tom 58

|

nr 2

19-32

In this study the pathogenicity of Rhizoctonia spp. isolates towards wheat seedlings in laboratory and greenhouse conditions was evaluated. In both experiments seven features were examined: plant height, roots weight, the percentage of infected stems and leaf sheaths and also the degree of stem and leaf sheaths infection. Isolates R1, R29, R39 and R59 were the most pathogenic. Percentage of infected stems ranged from 25.3 to 82.5 and roots from 35 to 82.3. The amplification of internal transcribed spacer regions (ITS1 and ITS2) between 18S, 5.8S and 28S rRNA genes and sequence analysis of these regions have been shown to be sufficiently variable to resolve two Rhizoctonia species. Random amplified polymorphic DNA (RAPD) was used to assess genetic variability among isolates. The suitability of RAPD method for isolates differentiation at intraspecific level was shown. Using seven arbitrary primers in polymerase chain reaction (PCR) thirty-three RAPD markers were generated. Clustering analysis from RAPD data resolved two groups of R. cerealis isolates at the 36% similarity level. Moreover, significant associations between molecular markers and pathogenicity of R. cerealis isolates were found.

11

Application of molecular techniques to taxonomic studies

80%

Lesicki A., Szweykowska-Kulinska Z.

Folia Malacologica

|

1999

|

tom 07

|

nr 4

12

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

80%

Alam-Eldin Y. H., Aaty H. E. A., Ahmed M. A.

Acta Parasitologica

|

2015

|

tom 60

|

nr 4

13

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

80%

Breda E., Wolny E., Hasterok R.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 4

The genus Brachypodium has become the target of extensive cytomolecular studies since one of its representatives, B. distachyon, has been accepted as a model plant for temperate cereals and forage grasses. Recent preliminary studies suggested that intraspecific rDNA polymorphism can occur in at least two members of the genus, B. sylvaticum and B. pinnatum, so the aim of this study was to further analyse this phenomenon. FISH with 25S rDNA and 5S rDNA probes was performed on somatic metaphase chromosomes, supplemented by the silver staining technique which distinguishes transcriptionally active from inactive 18S-5.8S-25S rDNA loci. The number, size and chromosomal distribution of 5S rDNA loci were very constant: two loci were invariably observed in all studied diploid accessions of both species, while four 5S rDNA loci were present in the tetraploid B. pinnatum. In contrast to 5S rDNA loci, those of the 35S rDNA were more variable. Two or three loci were observed in the diploid B. pinnatum and four in tetraploid accessions. In chromosome complements of B. sylvaticum accessions from two to six 35S rDNA sites were detected. Regardless of total rDNA locus number, only two were transcriptionally active in diploid accessions of both species, while two or four were active in the tetraploid B. pinnatum. Additionally, the fluorescent CMA/DAPI banding method was used to identify the relation between rDNA sites and CMA+ bands. It was revealed that the number and chromosomal distribution of CMA+ bands are in congruence only with 35S rDNA loci which gave strong FISH signals.

14

Molecular characterisation of Bulinus snails - intermediate hosts of schistosomes in Ogun State, south-western Nigeria

80%

Akinwale O., Oso O., Salawu O., Odaibo A., Tang P., Chen T.-W., Gyang P.

Folia Malacologica

|

2015

|

tom 23

|

nr 2

15

FISH mapping of 18S-5.8S-26S rRNA genes and fluorochrome banding in the triploid viviparous onion Allium x cornutum Clementi ex Visiani, 1842

80%

Lepen I., Puizina J.

Acta Biologica Cracoviensia. Series Botanica

|

2011

|

tom 53

|

nr 1

Triploid viviparous onions [Allium × cornutum Clementi ex Visiani 1842, syn Allium cepa L. var. viviparum Metzg. (ALEF.), auct.] (2n = 3x = 24), are known in some countries only as rare relict crops. In other parts of the world they are still traditionally or even commercially cultivated. In previous cytogenetic studies of the Croatian triploid viviparous onion Ljutika, Giemsa C-banding, chromosome pairing analysis during meiosis, and genomic hybridization in situ indicated a complex hybrid with highly heterozygous karyotype structure, with possible triparental genome organization. This study continues an analysis of the karyotype structure of Ljutika. Staining with fluorochromes CMA3 (Chromomycin A3) and DAPI (4,6-diamidino-2-phenylindole) confirmed previous results from Giemsa C-banding and revealed GC-rich heterochromatic regions associated mainly with chromosome ends and nucleolus organizing regions (NORs), and only a few interstitial bands. FISH mapping of the ribosomal 18S-5.8S-26S genes revealed two major rDNA signals on the short arms of two subtelocentric satellite chromosomes in almost all metaphase plates of Ljutika. The largest subtelocentric chromosome lacked rDNA signals. A significantly smaller rDNA signal was occasionally located on one small submetacentric chromosome. These results are in agreement with previously published results from identification of NORs by silverstaining technique, which confirmed a maximum three nucleoli in interphase nuclei. We discuss the molecular mechanisms underlying rearrangements and activity of ribosomal genes in the triploid karyotype.

16

rRNA genes - their distribution and activity in plants

80%

Maluszynska J., Hasterok R., Weiss H.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

|

1998

|

tom 1696

17

Phylogenetic position and ultrastructure of two Dermocystidium species [Ichthyosporea] from the common perch [Perca fluviatilis]

80%

Pekkarinen M., Lom J., Murphy C. A., Ragan M. A., Dykova I.

Acta Protozoologica

|

2003

|

tom 42

|

nr 4

18

Maize TF IIIA - the first transcription factor IIIA from monocotyledons. Purification and properties

80%

Wyszko E., Radlowski M., Bartkowiak S., Barciszewska M. Z.

Acta Biochimica Polonica

|

1997

|

tom 44

|

nr 3

Purification and properties of transcription factor IIIA (TF IIIA) from maize pollen (Zea mays L.) are presented for the first time. The purified protein has a molecular mass of about 35 kDa and exibits binding affinity toward both 5S rRNA and 5S rRNA gene. It also facilitates transcription of the 5S rRNA gene in a HeLa cell extract.

19

Sequential silver staining and fish - their use to distinguish active from inactive rDNA loci

80%

Hasterok R., Maluszynska J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

|

1998

|

tom 1696

Ograniczanie wyników

Prevalence and genotypes of Enterocytozoon bieneusi in sika deer in Jilin province, Northeastern China

Number and size of nucleoli in the spermatocytes of chicken and Japanese quail

Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification

Organization of the 18S, 5S, 4S rRNA genes and the tRNA-like repeat in the mitochondrial genomes of three lupin species

Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction

Prevalence of cdt-III gene encoding cytolethal distending toxin [CDT] among Escherichia coli strains isolated from pigs with diarrhea

Comparison of the nuclear organiser region activity in four taxa of the family Canidae

Phylogenetic positions of two ciliates, Paranophrys magna and Mesanophrys carcini [Ciliophora: Oligohymenophorea], within the subclass Scuticociliatia inferred from complete small subunit rRNA gene sequences

Application of multiplex PCR in routine microbiological diagnostics of Listeria monocytogenes and Listeria sp. strains in a meat-processing plant

Use of molecular and conventional techniques to identify and analyze genetic variability of Rhizoctonia spp. isolates

Application of molecular techniques to taxonomic studies

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

Molecular characterisation of Bulinus snails - intermediate hosts of schistosomes in Ogun State, south-western Nigeria

FISH mapping of 18S-5.8S-26S rRNA genes and fluorochrome banding in the triploid viviparous onion Allium x cornutum Clementi ex Visiani, 1842

rRNA genes - their distribution and activity in plants

Phylogenetic position and ultrastructure of two Dermocystidium species [Ichthyosporea] from the common perch [Perca fluviatilis]

Maize TF IIIA - the first transcription factor IIIA from monocotyledons. Purification and properties

Sequential silver staining and fish - their use to distinguish active from inactive rDNA loci