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Role of NAD[P]H: quinone oxidoreductase [NQO1] in apoptosis induction by aziridinylbenzoquinones RH1 and MeDZQ

100%

Nemeikaite-Ceniene A., Dringeliene A., Sarlauskas J., Cenas N.

Acta Biochimica Polonica

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2005

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tom 52

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nr 4

We aimed to characterize the role of NAD(P)H : quinone oxidoreductase (NQO1) in apoptosis induction by antitumour quinones RH1 (2,5-diaziridinyl-3-hydroxymethyl-6-methyl-1,4-benzoquinone) and MeDZQ (2,5-dimethyl-3,6-diaziridinyl-1,4-benzoquinone). Digitonin-permeabilized FLK cells catalyzed NADPH-dependent single- and two-electron reduction of RH1 and MeDZQ. At equitoxic concentrations, RH1 and MeDZQ induced apoptosis more efficiently than the nonalkylating duroquinone or H2O2. The antioxidant N,N'-diphenyl-p-phenylene diamine, desferrioxamine, and the inhibitor of NQO1 dicumarol, protected against apoptosis induction by all compounds investigated, but to a different extent. The results of multiparameter regression analysis indicate that RH1 and MeDZQ most likely induce apoptosis via NQO1-linked formation of alkylating species but not via NQO1-linked redox cycling.

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Oligonol prevented the relapse of dextran sulfate sodium-ulcerative colitis through enhancing Nrf2-mediated antioxidative defense mechanism

84%

Kim K.-J., Park J.-M., Lee J.-S., Kim Y. S., Kangwan N., Han Y.-M., Kang E. A., An J. M., Park Y. K., Hahm K.-B.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 3

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Two-electron reduction of quinones by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships

84%

Miseviciene L., Anusevicius Z., Sarlauskas J., Harris R. J., Scrutton N. S., Cenas N.

Acta Biochimica Polonica

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2007

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tom 54

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nr 2

In order to clarify the poorly understood mechanisms of two-electron reduction of quinones by flavoenzymes, we examined the quinone reductase reactions of a member of a structurally distinct old yellow enzyme family, Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase (PETNR). PETNR catalyzes two-electron reduction of quinones according to a 'ping-pong' scheme. A multiparameter analysis shows that the reactivity of quinones increases with an increase in their single-electron reduction potential and pKa of their semiquinones (a three-step (e-,H+,e-) hydride transfer scheme), or with an increase in their hydride-transfer potential (E7(H-)) (a single-step (H-) hydride transfer scheme), and decreases with a decrease in their van der Waals volume. However, the pH-dependence of PETNR reactivity is more consistent with a single-step hydride transfer. A comparison of X-ray data of PETNR, mammalian NAD(P)H: quinone oxidoreductase (NQO1), and Enterobacter cloacae nitroreductase, which reduce quinones in a two-electron way, and their reactivity revealed that PETNR is much less reactive, and much less sensitive to the quinone substrate steric effects than NQO1. This may be attributed to the lack of π-π stacking between quinone and the displaced aromatic amino acid in the active center, e.g., with Phe-178' in NQO1.

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Host nuclear factor erythroid 2-related factor 2 defense system determines the outcome of dextran sulfate sodium - induced colitis in mice

67%

Lee J. S., An J. M., Kang E. A., Han Y. M., Kim Y. S., Lee H. J., Kim K.-J., Surh Y. J., Hahm K.-B.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 5

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Journal of Physiology and Pharmacology

2 An J. M.

2 Cenas N.

2 Hahm K.-B.

2 Kang E. A.

2 Kim K.-J.

2 Kim Y. S.

2 Sarlauskas J.

1 Anusevicius Z.

1 Dringeliene A.

1 Han Y. M.

1 Han Y.-M.

1 Harris R. J.

1 Kangwan N.

1 Lee H. J.

1 Lee J. S.

1 Lee J.-S.

1 Miseviciene L.

1 Nemeikaite-Ceniene A.

1 Park J.-M.

1 Park Y. K.

1 Scrutton N. S.

1 Surh Y. J.

2 2018

1 2007

1 2005

Role of NAD[P]H: quinone oxidoreductase [NQO1] in apoptosis induction by aziridinylbenzoquinones RH1 and MeDZQ

Oligonol prevented the relapse of dextran sulfate sodium-ulcerative colitis through enhancing Nrf2-mediated antioxidative defense mechanism

Two-electron reduction of quinones by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships

Host nuclear factor erythroid 2-related factor 2 defense system determines the outcome of dextran sulfate sodium - induced colitis in mice