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1
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Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture

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Kaszuba-Zwoinska J., Chorobik P., Juszczak K., Zaraska W., Thor P. J.
Journal of Physiology and Pharmacology
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2012
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tom 63
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nr 5
2
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Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line

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Kaszuba-Zwoinska J., Wojcik K., Bereta M., Ziomber A., Pierzchalski P., Rokita E., Marcinkiewicz J., Zaraska W., Thor P.
Journal of Physiology and Pharmacology
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2010
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tom 61
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nr 2
Aim of study was to verify whether pulsating electromagnetic field (PEMF) can affect cancer cells proliferation and death. U937 human lymphoid cell line at densities starting from 1x106 cells/ml to 0.0625x106 cells/ml, were exposed to a pulsating magnetic field 50Hz, 45±5 mT three times for 3 h per each stimulation with 24 h intervals. Proliferation has been studied by counting number of cells stimulated and non-stimulated by PEMF during four days of cultivation. viability of cells was analyzed by APC labeled Annexin V and 7-AAD (7-amino-actinomycin D) dye binding and flow cytometry. Growing densities of cells increase cell death in cultures of U937 cells. PEMF exposition decreased amount of cells only in higher densities. Measurement of Annexin V binding and 7-AAD dye incorporation has shown that density-induced cell death corresponds with decrease of proliferation activity. PEMF potentiated density-induced death both apoptosis and necrosis. The strongest influence of PEMF has been found for 1x106cells/ml and 0.5x106 cells/ml density. To eliminate density effect on cell death, for further studies density 0.25x106 cells/ml was chosen. Puromycin, a telomerase inhibitor, was used as a cell death inducer at concentration 100 µg/ml. Combined interaction of three doses of puromycin and three fold PEMF interaction resulted in a reduced of apoptosis by 24,7% and necrosis by 13%. PEMF protects U937 cells against puromycin- induced cell death. PEMF effects on the human lymphoid cell line depends upon cell density. Increased density induced cells death and on the other hand prevented cells death induced by puromycin.
3
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Pulmonary surfactant: ultrastructural features and putative mechanisms of aging

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Walski M., Pokorski M., Antosiewicz J., Rekawek A., Frontczak-Baniewicz M., Jernajczyk U., Di Giulio C.
Journal of Physiology and Pharmacology. Supplement
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2009
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tom 60
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nr 5
4
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Changes of U937 cell viability induced by stress factors - possible role of calmodulin

100%
Wojcik-Piotrowicz K., Kaszuba-Zwoinska J., Rokita E., Nowak B., Thor P.
Journal of Physiology and Pharmacology
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2017
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tom 68
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nr 4
5

Rhythm of sporulation within the section Penicillium clavigerum. III. Induction of growth and zonation rhythms in Penicillium isariiforme Stolk and Meyer 247.56

88%
Piskorz-Binczycka B.
Acta Biologica Cracoviensia. Series Botanica
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1994
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tom 36
In Penicilium isariiforme there occur two endogenous rhythms: the growth-“wave” rhythm and sporulation rhythm in the form of coremia-bearing zonations. The growth rhythm of wave type occurring in P. isariiforme in light and in darkness is an endogenous spontaneous rhythm, whereas the sporulation rhythm manifested by the formation of spore-bearing coremia zonations represents an analogue induced rhythm. For this rhythm to occur a light impulse is necessary. The growth-wave rhythm has a long period 120 h, the sporulation rhythm has a 30 h period. Asparagine seems to regulate the sporulation rhythm; but it does not exert any greater influence on the wave rhythm in cultures grown in light nor in darkness. An addition of asparagine shortened the period of first zonation by a few hours, and the successive sporulation periods followed within very short time. Inhibitors such as: avidin. chloramphenicol, cycloheximide and puromycine controlled the length of the period of the sporulation rhythm.
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