Background. Pullulan is a water-soluble polysaccharide produced by fungi Aureobasidium pullulans. This glucan was applied for coating of food products. The aim of this study was obtaining of a pullulan coating and checking its effect on growth of microorganisms responsible for spoilage of food. Materials and methods. Pullulan produced by the white mutant A. pullulans B-1 was applied. Permeability of oxygen and carbon dioxide through film produced from 10% water solution of pullulan was checked as well as degree of inhibition of chosen microorganisms through pullulan coating formed on surface of growth‟s media. Results. Low permeability of gases through pullulan film and a considerable growth‟s limitation of all tested microorganisms were found. A total growth‟s inhibition of 21 from 36 tested strains and a partial growth‟s limitation of the remaining 15 strains was observed. The inhibitory effect was diverse and it was from 63 to 100%. Conclusions. These results proved that pullulan coating applied in these tests revealed big barrier characteristics in relation to oxygen and carbon dioxide, which had effect upon growth‟s inhibition of most of the tested microorganisms, responsible for spoilage of food