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The aim of the study was to identify the species of microorganisms isolated from the uterus of healthy cows (control group) and cows affected with puerperal metritis (PM) before and after an experimental therapy with an immunomodulator and antibiotics versus commonly applied methods (antibiotic + ß-blocker or antibiotic + PGF2a). Examinations were carried out on 110 cows with PM in three farms with similar system of rearing and nutrition. The control group consisted of 21 cows without postpartum disturbances. Smears from the uterus were taken before treatment and then at 21st day of observation. Escherichia coli and other species of Enterobacteriaceae family were isolated from 48.2%of PM cows and 47.6% of healthy cows. The degree of Arcanobacterium pyogenes infection was statistically lower in healthy than in sick cows (9.5% versus 30.0%). Streptococcus sp. was isolated from 13.6% of PM cows and from 16% of control ones. Staphylococci were isolated from 10% of PM and from 30% of control cows. Other bacteria species were isolated from about 10% of the examined cows. The best elimination of infections (66%) was noted in cows treated with the intrauterine antibiotic in combination with PGF2a i.m. injection. Examination showed that species of bacteria in the postparturient uterus were similar in healthy and sick cows. However, Arcanobacterium pyogenes was isolated 3 times more often from the sick animals. This pathogen was identified in 11 cows out of 19 (57.9%) culled subsequently because of infertility after the metritis puerperalis was clinically cured.
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Predisposing factors for puerperal metritis in cows

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The investigation was performed on 90 multiparous cows in four farms. Animals were almost identically fed and had similar milk yield during last year (6000 kg milk). Blood samples were taken 3 times (-3, -2, -1 week) before and 2 times (+1, +3 week) after parturition. Some biochemical parameters were determined. In all farms the increase of free fatty acids level was observed after parturition. The decrease in cholesterol concentration and triglyceride level after calving were accompanied. Differences in valucs of analysed parameters (AST, FFA, glucose) in cows with correct and disturbed post partum period were observed before parturition.
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