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1

LH release in response to GnRH in cows with puerperal endometritis

100%
Janowski T., Chmielewski A., Jaroszewski J., Zdunczyk S.
Polish Journal of Veterinary Sciences
|
1998
|
tom 01
|
nr 1
Ten cows with and 5 cows without puerperal endometritis were treated with 20 μg GnRH analogue, buserelin, intramuscularly. Blood samples were taken every 20 min for 8h before and for 8h after injection of GnRH. All cows responded to GnRH with significant increase of LH 20 min after treatment. The LH peak was significantly lower in cows with puerperal endometritis than that in clinically normal cows (27.91 ± 5.47 ng/ml vs 80.60 ± 17.2 ng/ml; p ≤ 0.01). The total AUC of LH was also significantly smaller in cows with puerperal endometritis compared to cows without it (p ≤ 0,01). The results suggest that the delayed resumption of ovarian function in cows with uterine infection may be caused by reduced responsiveness of the pituitary to endogenous GnRH. Further studies are needed in order to investigate the mechanism of this phenomenon.
2

Influence of various treatment methods on bacteriological findings in cows with puerperal endometritis

100%
Kaczmarowski M., Malinowski E., Markiewicz H.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 3
The aim of the study was to identify the species of microorganisms isolated from the uterus of healthy cows (control group) and cows affected with puerperal metritis (PM) before and after an experimental therapy with an immunomodulator and antibiotics versus commonly applied methods (antibiotic + ß-blocker or antibiotic + PGF2a). Examinations were carried out on 110 cows with PM in three farms with similar system of rearing and nutrition. The control group consisted of 21 cows without postpartum disturbances. Smears from the uterus were taken before treatment and then at 21st day of observation. Escherichia coli and other species of Enterobacteriaceae family were isolated from 48.2%of PM cows and 47.6% of healthy cows. The degree of Arcanobacterium pyogenes infection was statistically lower in healthy than in sick cows (9.5% versus 30.0%). Streptococcus sp. was isolated from 13.6% of PM cows and from 16% of control ones. Staphylococci were isolated from 10% of PM and from 30% of control cows. Other bacteria species were isolated from about 10% of the examined cows. The best elimination of infections (66%) was noted in cows treated with the intrauterine antibiotic in combination with PGF2a i.m. injection. Examination showed that species of bacteria in the postparturient uterus were similar in healthy and sick cows. However, Arcanobacterium pyogenes was isolated 3 times more often from the sick animals. This pathogen was identified in 11 cows out of 19 (57.9%) culled subsequently because of infertility after the metritis puerperalis was clinically cured.
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