The aim of this study was to determine of seroprevalence of bluetongue (BT) virus specific antibodies in susceptible animals imported into Poland after 15 June 2006 from Germany, the Netherlands, France and Belgium. From 1 September to 31 December 2006, 5757 samples of sera supplied from 16 voivodeships were tested by c-ELISA, and positive results were confirmed using agar gel immunodiffusion (AGID). Out of all tested sera, only 3 samples from Zachodniopomorskie Voivodeship were found to be positive for antibodies to BTV (0.05% of the total number of sera samples). The seroreagents were held on a farm in the Swidwin district but their country of origin was the Netherlands. No virus was detected by RT-PCR in EDTA blood samples taken from seroreagents. The results obtained in the National Veterinary Research Institute confirmed by the Community Reference Laboratory for BT in Pirbright (England), where antibodies against BTV-8 were detected in the supplied sera samples.
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