Wyniki wyszukiwania

1

Pre-eclampsia-associated alterations in Wharton's jelly proteoglycans

100%

Gogiel T., Galewska Z., Jaworski S.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 2

Proteoglycans of Wharton’s jelly contain mainly chondroitin/dermatan sulphate chains. The predominant proteoglycan is decorin (core proteins of 45 and 47 kDa), although the core proteins of biglycan (45 kDa), versican (260 kDa) and of other proteoglycans (90, 110, 220 kDa) were also detected (Gogiel et al., 2003). The aim of the present study was to compare the proteoglycan composition of Wharton’s jelly of newborns delivered by healthy mothers and those with pre-eclampsia. Proteoglycans from pre-eclamptic Wharton’s jelly had a higher sulphated glycosaminoglycan/protein ratio than those of normal tissue. Pre-eclampsia is associated with a lower level of all proteoglycan core proteins, especially those of higher molecular mass (such as versican), although the same set of core proteins were found in normal and pre-eclamptic Wharton’s jelly. The alterations in the proteoglycan composition of Wharton’s jelly may affect the mechanical properties of the umbilical cord and, in the case of pre-eclampsia, disturb foetal blood circulation.

2

The changes of proteoglycans during the hair cycle in the mink skin

100%

Pak J., Nakamura F., Kondo K.

Zeszyty Naukowe. Przegląd Hodowlany

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1996

|

tom 29

3

Biglycan is internalized via a chlorpromazine-sensitive route

100%

Gotte M., Feugaing D. D. S., Kresse H.

Cellular and Molecular Biology Letters

|

2004

|

tom 09

|

nr 3

The small leucine-rich proteoglycan biglycan (BGN) is abundantly expressed in mesenchymal tissues. Its expression level is related to the phenotypic differentiation of cells. A dysregulation in BGN expression occurs under several pathological conditions, including glomerulonephritis, mesothelioma, pancreatic cancer and a mouse model of osteoporosis. Since the extracellular concentration of BGN is regulated both by secretion and endocytosis, we performed mechanistic studies on BGN endocytosis in human skin fibroblasts in vitro, using inhibitors of different endocytic routes. Chlorpromazine, an inhibitor of the clathrin-coated pit-pathway reduced endocytosis of BGN in human skin fibroblasts by 40%, and decreased degradation of BGN by 66%. Filipin, an inhibitor of the caveolae pathway, and Tyrphostin AG 1478, a specific inhibitor of EGF-receptor phosphorylation that partially inhibits endocytosis of the structurally related proteoglycan decorin, had no influence on BGN internalization and degradation. Our data indicates that the classical clathrin-mediated endocytic pathway is a major route for the internalization of BGN. Based on the differential susceptibility to pharmacological inhibition, it appears that BGN endocytosis seems to be at least in part mechanistically different from decorin uptake.

4

Proteoglycans of human umbilical cord arteries

86%

Gogiel T., Jaworski S.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 4

Proteoglycans (PGs) were dissociatively extracted from human umbilical cord arteries (UCAs) with 4 M guanidine hydrochloride containing Triton X-100 and protease inhibitors, purified by Q-Sepharose anion exchange chromatography and lyophilized. They were analysed by gel filtration, SDS/PAGE and agarose gel electrophoresis before and after treatment with chondroitinase ABC. It was found that the PG preparation was especially enriched in chondroitin/dermatan sulphate PGs. The predominant PG fraction included small PGs that emerged from Sepharose CL-2B with Kav = 0.74. Their molecular mass, estimated by SDS/PAGE, was 160-200 kDa and 90-150 kDa, i.e. it was typical for biglycan and decorin, respectively. Treatment with chondroitinase ABC yielded the core proteins of 45 and 47 kDa, characteristic for both small PGs. Remarkable amounts of the 45 kDa protein were detected in non-treated PG samples, suggesting the presence of free core proteins of biglycan and decorin. Large PGs were present in lower amounts. In intact form they were eluted from Sepharose CL-2B with Kav = 0.17 and 0.43. Digestion with chondroitinase ABC yielded the core proteins with a molecular mass within the range of 180-360 kDa but predominant were the bands of 200, 250 and 360 kDa. The large PGs probably represent various forms of versican or perlecan bearing chondroitin sulphate chains.

5

Identification of arabinogalactan-proteins [AGPs] in sugar beet suspension cultures

86%

Munster A., Majewska-Sawka A., De Dios Alche Ramirez J.

Folia Morphologica

|

1998

|

tom 57

|

nr 1

6

The role of heparanase in diseases of the glomeruli

72%

Szymczak M., Kuzniar J., Klinger M.

Archivum Immunologiae et Therapiae Experimentalis

|

2010

|

tom 58

|

nr 1

45-56

7

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity

72%

Minarowska A., Minarowski L., Karwowska A., Milewska A. J., Gacko M.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 1

8

Dermatan sulfate remodeling associated with advanced Dupuytren's contracture

72%

Kozma E. M., Glowacki A., Olczyk K., Ciecierska M.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 4

821-830

Dermatan sulfate (DS) widespread as a component of extracellular matrix proteoglycans, is characterized by great bio-reactivity and remarkable structural heterogeneity due to distinct degrees of sulfation and glucuronosyl epimerization and different polymerization degrees. However, DS metabolism under various biological conditions is poorly known. Dupuytren’s contracture is a benign fibromatosis leading to complex remodeling of the palmar fascia structure and properties. However, it remains unclear whether the disease affects the structure of DS, which is the major tissue glycosaminoglycan. Thus the aim of the study was to examine the structure of the total DS in Dupuytren’s fascia. DS chains were extracted from 5 samples of normal fascia and 7 specimens of Dupuytren’s tissue by papain digestion followed by fractionation with cetylpyridinium chloride. Then, DS structure analysis was performed comprising the evaluation of its molecular masses and sensitivity to hyaluronidase and chondroitinase B. Dupuytren’s contracture is associated with significant remodeling of DS chain structure revealed by (1) a distinct profile of chain molecular masses characterized by the appearance of long size components as well as the increase in the content of small size chains; (2) a different glucuronosyl epimerization pattern connected with the enhanced content of glucuronate disaccharide blocks; (3) chain oversulfation. These structural alterations in total DS may modify the GAG interactions especially affecting collagen fibrillogenesis and growth factor availability. Thus, Dupuytren’s contracture associated DS remodeling may promote the phenomena typical for advanced disease: apoptosis and reduction in cell number as well as the appearance of dense pseudotendinous collagen matrix

9

Immunodetection of arabinogalactan proteins (AGPs) in apomictic ovules of Chondrilla juncea L.

72%

Chudzik B., Koscinska-Pajak M., Sniezko R.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2005

|

tom 47

|

nr 1

10

Expression of syndecan-1 and cathepsins D and K in advanced esophageal squamous cell carcinoma

72%

Szumilo J., Burdan F., Zinkiewicz K., Dudka J., Klepacz R., Dabrowski A., Korobowicz E.

Folia Histochemica et Cytobiologica

|

2009

|

tom 47

|

nr 4

11

Synthesis of proteoglycans in tissue explants derived from aortas of rabbits with experimentally induced atherosclerosis

72%

Zak I., Skop B.

Acta Biochimica Polonica

|

1993

|

tom 40

|

nr 1

12

Wplyw fluorkow na metabolizm komorek tkanki lacznej

58%

Olszowski T., Chlubek D.

Bromatologia i Chemia Toksykologiczna

|

2002

|

tom 35

|

nr 1

27-30

13

Immunocytochemiczna lokalizacja epitopów pektyn i proteoglikanów AGP w regenerujących tkankach buraka cukrowego (Beta vulgaris L.)

58%

Tomaszewska-Sowa M.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2011

|

nr 259

14

Hydrogen peroxide as a potential mediator of the transcriptional regulation of heparan sulphate biosynthesis in keratinocytes

58%

Nakayama F., Hagiwara A., Yamamoto T., Akashi M.

Cellular and Molecular Biology Letters

|

2008

|

tom 13

|

nr 3

475-492

Ionizing radiation is one of the types of oxidative stress that has a number of damaging effects on cutaneous tissues. One of the histological features of radiation-induced cutaneous fibrosis is the accumulation of extracellular matrix (ECM) components, including heparan sulfate proteoglycan (HSPG), which are required for the repair of tissue damage, and operate by interacting with a variety of growth factors. In this study, we established a model of human HaCaT keratinocytes overexpressing anti-oxidative enzyme genes to elucidate the mechanism of oxidative stress leading to the accumulation of HSPG and the role of its accumulation. Catalase overexpression induced an increase in anti-HS antibody (10E4) epitope expression in these cells. Western blotting showed that the smeared bands of HSPG were obviously shifted to a higher molecular weight in the catalase transfectants due to glycosylation. After heparitinase I treatment, the core proteins of HSPG were expressed in the catalase transfectants to almost the same extent as in the control cells. In addition, the transcript levels of all the enzymes required for the synthesis of the heparan sulfate chain were estimated in the catalase transfectant clones. The levels of five enzyme transcripts — xylosyltransferase-II (XT-II), EXTL2, D-glucuronyl C5-epimerase (GLCE), HS2-O-sulfotransferase (HS2ST), and HS6-O-sulfotransferase (HS6ST) — were significantly increased in the transfectants. Moreover, hydrogen peroxide was found to down-regulate the levels of these enzymes. By contrast, siRNA-mediated repression of catalase decreased 10E4 epitope expression, the transcript level of HS2ST1, and the growth rate of HaCaT cells. These findings suggested that peroxide-mediated transcriptional regulation of HS metabolism-related genes modified the HS chains in the HaCaT keratinocytes.

15

Desmosealin and other components of the epidermal extracellular matrix

58%

Sandjeu Y., Haftek M.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 4

16

Morphology and physiology of the epiphyseal growth plate

58%

Burdan F., Szumilo J., Korobowicz A., Farooquee R., Patel S., Patel A., Dave A., Szumilo M., Solecki M., Klepacz R., Dudka J.

Folia Histochemica et Cytobiologica

|

2009

|

tom 47

|

nr 1

5-16

17

Lumican inhibits B16F1 melanoma cell lung metastasis

58%

Brezillon S., Zeltz C., Schneider L., Terryn C., Vuillermoz B., Ramont L., Perreau C., Pluot M., Diebold M. D., Radwanska A., Malicka-Blaszczkiewicz M., Maquart F.-X., Wegrowski Y.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 4

18

Syndecan signaling: when, where and why?

58%

Multhaupt H. A. B., Yoneda A., Whiteford J. R., Oh E.-S., Lee W., Couchman J. R.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 4

19

Alcohol abuse and glycoconjugate metabolism

58%

Waszkiewicz N., Szajda S. D., Zalewska A., Szulc A., Kepka A., Minarowska A., Wojewodzka-Zelezniakowicz M., Konarzewska B., Chojnowska S., Ladny J. R., Zwierz K.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 1

20

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Glucosamine in serum of patients after myocardial infarction subjected to rehabilitation training

58%

Nowak A., Szczesniak L., Rychlewski T., Dylewicz P., Karolkiewicz J.

Journal of Physiology and Pharmacology

|

1998

|

tom 49

|

nr 2

Ograniczanie wyników

Pre-eclampsia-associated alterations in Wharton's jelly proteoglycans

The changes of proteoglycans during the hair cycle in the mink skin

Biglycan is internalized via a chlorpromazine-sensitive route

Proteoglycans of human umbilical cord arteries

Identification of arabinogalactan-proteins [AGPs] in sugar beet suspension cultures

The role of heparanase in diseases of the glomeruli

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity

Dermatan sulfate remodeling associated with advanced Dupuytren's contracture

Immunodetection of arabinogalactan proteins (AGPs) in apomictic ovules of Chondrilla juncea L.

Expression of syndecan-1 and cathepsins D and K in advanced esophageal squamous cell carcinoma

Synthesis of proteoglycans in tissue explants derived from aortas of rabbits with experimentally induced atherosclerosis

Wplyw fluorkow na metabolizm komorek tkanki lacznej

Immunocytochemiczna lokalizacja epitopów pektyn i proteoglikanów AGP w regenerujących tkankach buraka cukrowego (Beta vulgaris L.)

Hydrogen peroxide as a potential mediator of the transcriptional regulation of heparan sulphate biosynthesis in keratinocytes

Desmosealin and other components of the epidermal extracellular matrix

Morphology and physiology of the epiphyseal growth plate

Lumican inhibits B16F1 melanoma cell lung metastasis

Syndecan signaling: when, where and why?

Alcohol abuse and glycoconjugate metabolism

Glucosamine in serum of patients after myocardial infarction subjected to rehabilitation training