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Application of electrophoretic methods for detection of protein-porphyrin complexes

100%

Kowalska A., Kwiek P., Milosz E., Gondek G., Romiszewska A., Graczyk A., Podhajska A. J.

Acta Biochimica Polonica

2003

tom 50

nr 4

Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc™ 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Gondek G.

1 Graczyk A.

1 Kowalska A.

1 Kwiek P.

1 Milosz E.

1 Podhajska A. J.

1 Romiszewska A.

1 2003

Wyniki wyszukiwania

Application of electrophoretic methods for detection of protein-porphyrin complexes