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maf1 Mutation alters the subcellular localization of the Mod5 protein in yeast

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Murawski M., Szczesniak B., Zoladek T., Hopper A. K., Martin N. C., Boguta M.

Acta Biochimica Polonica

1994

tom 41

nr 4

Two forms of Mod5p, a tRNA modification enzyme, are found in three intracellular compartments, mitochondria, cytoplasm and nucleus, but arc encoded by a single MOD5 gene. The two forms of the enzyme, Mod5p-I and Mod5p-II differ at the N-termini and are produced by initiation of translation at different start codons. Mod5p-I does contain a mitochondrial targeting signal and is distributed between mitochondria and cytoplasm, whereas Mod5p-II is found in the cytosol and nucleus (Boguta, M., et al. 1994, Mol. Cell. Biol. 14, 229&-2306). In the present work mutants which mislocalize the Mod5p-I enzyme were isolated. The screen was based on a correlation between the amount of cytosolic protein and the efficiency of tRNA mediated suppression. Identification of mutants is possible because a red pigment accumulates in the cells unable to suppress an ade2-l nonsense allele. The mafl mutant, with an altered intracellular localization of the Mod5p-I protein, was isolated. Immunofluorescence data suggest that the mutation causes mislocalization of the Mod5p-I to the nucleus.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Boguta M.

1 Hopper A. K.

1 Martin N. C.

1 Murawski M.

1 Szczesniak B.

1 Zoladek T.

1 1994

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maf1 Mutation alters the subcellular localization of the Mod5 protein in yeast