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1

Protein profiles from intact cells as a tool in Bifidobacterium characteristics

100%
Wasko A., Polak-Berecka M., Kalita M.
Polish Journal of Microbiology
|
2012
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tom 61
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nr 4
In this study sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) profiles were analysed and differences were confirmed by a unweighted pair group method with arithmetic average (UPGMA) analysis between bifidobacterial species, such as B. infanis ATCC1567, B. bifidum Bb-12, B. longum KN29, B. catenulatum KD14, and B. animalis BI30. Two dimensional electrophoresis separation profiles were compared, and the most characteristic spots were characterized by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We propose proteins extracted from intact cells as an additional trait for bifidobacteria characterization, together with molecular techniques, which can be used to analyze bacterial protein polymorphism and to distinguish among species.
2

Ultra- and nanofiltration of acid-whey - changes in whey proteins profile

86%
Baczek N., Soral-Smietana M., Wronkowska M., Zander L., Dajnowiec F., Banaszczyk P.
Polish Journal of Food and Nutrition Sciences
|
2011
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tom 61
|
nr Suppl.1
3

SDS-PAGE protein profiles of developing seeds of Abies concolor

86%
Kormutak A., Libiakova G., Salaj J., Libantova J., Moravcikova J., Vookova B.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2005
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tom 47
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nr 1
4

Jasmonic acid - methyl ester - induced protein profile modifications in wheat [Triticum aestivum L.]

86%
Rafi M. M., Zemetra R. S., Berger P. H.
Acta Physiologiae Plantarum
|
1996
|
tom 18
|
nr 1
5

Differentiated pattern of protein composition of crystalline inclusions of newly isolated Bacillus thuringiensis strains from Silesia in Poland

86%
Guz K., Kucinska J., Lonc E., Doroszkiewicz W.
Polish Journal of Microbiology
|
2005
|
tom 54
|
nr 4
Protein profiles of crystal delta-endotoxins were determined in twenty nine Bacillus thuringiensis strains-soil and phylloplane isolates - from Poland. Electrophoretic analysis revealed quantatively and qualitatively different patterns of delta-endotoxin crystal preparations of these B. thuringiensis strains. The crystalline parasporal inclusions of B. thuringiensis isolates were composed of two, three, four or five proteins. Molecular weights of these polypeptides varied from 23.4 kDa to 142 kDa. There is lack of correlation between serovars of B. thuringiensis strains, the morphology of crystals and the number and size of proteins in parasporal inclusions.
6

Differences between the Baltic cod Gadus morhua callarias L. and Atlantic cod Gadus morhua morhua L., by skeletal muscle protein polymorphism, analyzed with classical SDS-PAGE electrophoresis

72%
Antoszek A., Antoszek J., Wiecaszek B., Drozd R., Suszycki S.
Electronic Journal of Polish Agricultural Universities. Series Fisheries
|
2011
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tom 14
|
nr 3
7

Correlation between the proteins and protein profile(s) of different regions of epididymis and their contents in goat buck

72%
Cheema R. S., Bansal A. K., Bilaspuri G. S., Gandotra V. K.
Animal Science Papers and Reports
|
2011
|
tom 29
|
nr 1
Total proteins and their profile(s) in three regions of epididymis, its fluid and sperm membrane extract (SME) were analysed with an objective to determine the changes in spermatozoa during epididymal maturation in goat buck. The protein content from caput to cauda increased nonsignificantly (P>0.05) while significantly (P<0.05) in tissue homogenate and spermatozoa. SDSPAGE analysis indicated the removal of >205, 205 and 95 kDa; 40 kDa proteins only in the caput and corpus spermatozoa, respectively. The proteins with molecular weight of 10, 18, 25, 35 and 20 kDa, detected only in cauda and corpus spermatozoa, respectively, seem to be associated in the maturation process of spermatozoa during epididymal transit. SDS-Page analysis of tissue and fluid indicated that the >205, 205, 195, 200, 45, 25, 18, 15 and 12 kDa proteins are structural as well as secretory proteins because of their presence both in the epididymal tissue and fluid. The nature of 100, 97, 75 and 70 kDa was found to be purely structural because of their presence in the tissue only, whereas 90, 35, 6.5 and 3.0 kDa proteins, mainly of secretory nature, due to their detection only in the fluid. Therefore, the presence of 35, 25, 18 kDa proteins both in the epididymal fluid and cauda sperm indicate that these proteins are associated with goat buck sperm maturation and fertility. Therefore, it can be concluded that the proteins of 35, 25, and 18 kDa, associated with goat sperm maturation are similar to the mentioned molecular markers in other species and can be worked out as fertility markers for goat buck semen.
8

The changes in the proteins profile induced by cadmium stress in flax [Linum usitatissimum L.]

72%
Szalata M., Szalata M., Wielgus K.
Herba Polonica
|
2009
|
tom 55
|
nr 3
298-303
Influence of Cd(NO3)2 concentration on the process of flax (Linum usitatissimum L.) germination and changes occurring on the level of synthesized proteins or peptides were evaluated. The studies did not reveal differences in seed germination in control conditions and in presence of cadmium solution. Extracts obtained from two-week-old seedlings were exposed to ammonium sulphate saturation (0–30 and 50–80%). Ion exchange chromatography on DEAE Cellulose revealed appearance of proteins or peptides rich in the cysteine residues which were not present in control group (absorbance 280 and 254 nm). Particular proteins were observed in three extract fractions eluted by NaCl gradient (0.3–0.5 M NaCl) what could suggests formation of (Cys-rich) protein or peptides in presence of the cadmium solution during detoxification process. The largest absorbance indicated a high concentration of (Cys-rich) proteins or peptides related probably to incubation with cadmium solution observed at extract fraction eluted with 0.4 M NaCl.
9
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Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions

72%
Khezri M., Mohammadi M.
Journal of Plant Protection Research
|
2018
|
tom 58
|
nr 4
Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
10

The effect of temperature on midgut and brain protein profiles in Morimus funereus larvae [Coleoptera: Cerambycidae]

72%
Ilijin L., Jankovic-Tomanic M., Mitic M., Vlahovic M., Prolic Z., Nenadovic V.
Folia Biologica
|
2002
|
tom 50
|
nr 3-4
11

Isolation, characterization and phylogenetic analysis of halophilic archaea from a salt mine in Central Anatolia (Turkey)

58%
Yildiz E., Ozcan B., Caliskan M.
Polish Journal of Microbiology
|
2012
|
tom 61
|
nr 2
The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified on the basis of their morphological, physiological, biochemical properties, polar lipid and protein profiles and 16S rDNA sequences. From the 16S rDNA sequences comparisons it was established that the isolates CH2, CH3 and CHC resembled Halorubrum saccharovorum by 98.8%, 98.9% and 99.5%, respectively. There was a 99.7% similarity between the isolate CH11 and Halobacterium noricense and 99.2% between the isolate CHA1 and Haloarcula argentinensis. The isolate CH8K and CH8B revealed a similarity rate of 99.8% and 99.3% to Halococcus dombrowskii, respectively. It was concluded that the isolates named CH2, CH3 and CHC were clustered in the genus Halorubrum and that CHA1 and CH7 in the genus Haloarcula, CH8K and CH8B in the genus Halococcus and CH11 in the genus Halobacterium.
12

Proteomic analysis of plasma profiles in children with recurrent bone fractures

58%
Rusinska A., Swiatkowska M., Koziolkiewicz W., Skurzynski S., Golec J., Chlebna-Sokol D.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 4
The aim of the study is proteomic analysis of the plasma profile in children with recurrent bone fractures. The study involved 16 children: 6 patients with recurrent low-energy fractures and normal bone mass and 10 with osteogenesis imperfecta. In the analysis of the protein profile, the two-dimensional protein electrophoresis was used (Ettan DALT II, Amersham Bioscience). The images of protein gels were compared with controls. The protein spots with changed expression were cut from the gel and the amino acid sequence was analyzed with the mass spectrometry method (Q-Tof PremierTM API MASS SPECTROMETR, Waters) for protein identification. The most prevalent protein with changed expression, with respect to controls, was haptoglobin observed in 6 patients with a severe form of osteogenesis imperfecta. Increased haptoglobin concentration in these patients was confirmed by the ELISA method. Peptides corresponding to alpha-1 acid glycoprotein and serum amyloid P-component, apolipoprotein A-I, and transthyretin were detected in one, two and three children, respectively. Conclusions: 1) The results show increased haptoglobin which may be suggestive of an inflammatory component taking part in the course of osteogenesis imperfecta. 2) Further studies to explain the possible relationship of this protein with increased bone fragility are necessary.
13

Interspecific differences between Atlantic salmon and Arctic charr in susceptibility to infection with larval and adult Caligus elongatus: effect of skin mucus protein profiles and epidermal histological differences

58%
Mustafa A., Mackinnon B. M., Piasecki W.
Acta Ichthyologica et Piscatoria
|
2005
|
tom 35
|
nr 1
Background. Arctic charr, Salvelinus alpinus (L.), are known to harbour significantly more sea lice, Caligus elongatus von Nordmann, 1832, than do Atlantic salmon, Salmo salar L. This research investigated whether this is due to differences in initial infection or to resettlement/loss of mobile adults. Skin mucus protein profiles and epidermal histology were also studied as two characteristics that might help explain interspecific differences in host susceptibility. Materials and Methods. Atlantic salmon and Arctic charr were sampled from the sea-pen after 12 weeks of exposure to natural infection. Fishes were examined for sea lice counts and mucus was analysed for protein profiles using standard methods of poly-acrylamide gel electrophoresis. Epidermal tissue of fishes around chalimus attachment sites was also examined by transmission electron microscopy. Results. Interspecific differences in host susceptibility were determined not to be due to differences in initial infection rate, as charr and salmon had similar numbers of attached chalimi. Adult parasites were significantly more numerous on charr as a result of resettlement of adults lost by other fishes in the area. Gel electrophoresis indicated that salmon had a greater abundance of skin mucus proteins than charr, particularly in the 30–42 and 67–94 kDa range. Ultrastructural analysis of epidermal tissue indicated that charr had more mucous cells and showed evidence of possible osmotic stress. Interspecific differences also existed in intercellular adhesion characteristics. Salmon and charr showed a similar absence of inflammation around chalimus attachment sites. Conclusion. Artic charr had a more intense infection with Caligus elongatus than did salmon corresponding to fewer potentially antagonistic proteins in the mucus. Perhaps also, the epidermis of charr provides more easily accessible food for the sea lice. Higher levels of stress in charr in sea water may also have predisposed them to higher sea lice infections.
14

Changes in nitrogen content and protein profiles following in vitro selection of NaCl resistant mung bean and tomato

58%
Hassan N. M., Serag M. S., El-Feky F. M.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 2
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