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Protein digestibility may be Influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purifi ed rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only signifi cant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.
The aim of the study was to determine influence of oxidation degree on available lysine content and protein digestibility in meat products with added antioxidants (rosemary extracts, soy protein hydrolyzate, BHT) stored under frozen conditions.
The aim of the study was to determine the content and biological activity of phenolic compounds of three faba bean genotypes - a coloured flowered Nadwiślański variety, a coloured flowered "self-ending" Tinos variety and white flowered Caspar variety. Comparing the white flowered Caspar variety containing 0.34% of phenolic compounds in the seed coat, in this proantho- cyanidins made from 0.04%, the Polish Nadwiślański and Tinos variety were characterised as having a higher phenolic compound content in the seed coat, i.e. 8.70% and 11.75%, respectively. The proanthocyanidins content, being 6.54% and 8.32% respectively, is in accordance with the lower values determined in the seed coats of many European high-tannin faba bean varieties. Seed coats containing a higher level of polyphenols, added to casein diets in amounts of 15%, highly significantly lowered the digestibility and protein efficiency. Depending on the variety of seed coat, TD protein coefficients amounted to: Caspar — 85.4%, Nadwiślański — 71.3%, Tinos — 69.3%. For these same diets the NPU index was 71.2, 59.3 and 49.4, and the PER index was 2.63, 2.23 and 2.16, respectively. It was also found highly significant decrease of Ca, Fe, and Zn apparent digestibilities in casein diets with faba bean seed coats of a high polyphenolic content.
The aim of the study was to determine the influence of oxidation degree on available lysine and methionine content and protein digestibility in meat products with added antioxidants (rosemary extracts, green tea extracts, BHT) stored under frozen conditions. Oxidation degree of lipid using peroxide value (PV), anisidine value (AV), thiobarbituric acid reactive substances (TBARS), and Totox coefficient was controlled and protein digestibility and content of available lysine and methionine were determined periodically. Results showed an increase in lipid oxidation during storage. The highest values for the applied measurement of oxidation were observed in the control sample without antioxidants. The added antioxidants slowed down lipid oxidation to a significant extent. In control samples, the content of available lysine and methionine was reduced by 53% and 75% respectively, whereas protein digestibility by 12.5% at the end of storage. Inhibition of lipid oxidation products formation by the addition of antioxidants, limited significantly lysine and methionine losses and reduction of protein digestibility. The application of antioxidants extended stability and protected biological protein value of the meat products examined.
Effect of extrusion variables (temperature and moisture content) on amino acids, available lysine and in vitro protein digestibility of the extru- dates from pinto bean were studied. Chemical score (Cs) and essential amino acids (EAAI) indices were calculated on the basis of the amino acids composition of egg protein according to FAO/WHO. No significant changes in most amino acids were found between raw and extruded samples, except cystine and tryptophan. Their contents lowered significantly (p<0.05) under conditions of processing. Temperature and moisture content significantly reduced the available lysine content from 4.65g/16g N in the non-extruded samples to 3.90 and 3.60g/16g N for samples processed at 133 and 156°C, respectively. Extrusion process temperature improved significantly in vitro protein digestibility. Considerable decreases of indices were found: chemical score (Cs) from 46.7 to the range of 31.4-33.8 and essential amino acids (EAAI) from 77.8 to 71.9-72.6.
W pracy badano wpływ przedsiewnego zastosowania promieniowania gamma i ogrzewania mikrofalowego na zmiany w strawności rzeczywistej i pozornej albumin wyizolowanych z ziarna pszenicy I i II pokolenia. Napromienienie przed wysianiem w zakresie dawek 0,05 kGy i 0,1 kGy spowodowało istotny statystycznie wzrost zawartości białka całkowitego tylko w I pokoleniu. Także tylko w I pokoleniu odnotowano mniejszą strawność rzeczywistą i pozorną białek albuminowych wyekstrahowanych z ziarna napromienionego przed wysianiem, w porównaniu z próbą kontrolną. Potraktowanie ziarna mikrofalami przed wysiewem przez 15 s (28°C) do 180 s (98°C), spowodowało istotne statystycznie zmiany w ilości białka całkowitego tylko w I pokoleniu. Natomiast białka albuminowe wyekstrahowane z ziarna zarówno I jak i II pokolenia, wyhodowanego z nasion pszenicy potraktowanej mikrofalami przed wysiewem, charakteryzowały się niższą strawnością w stosunku do prób kontrolnych.
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