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1

Methionyl-tRNA synthetase

100%
Deniziak M. A., Barciszewski J.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 2
Methionyl-tRNA synthetase (MetRS) be longs to the fam ily of 20 en zymes es sen tial for pro tein biosynthesis. It links co va lently methionine with its cog nate tRNA. Crys tal structures solved for bac te rial MetRSs have given a num ber of in ter est ingin sights into en zyme ar chi tec ture and methionylation ca tal y sis. A com par i son of se quences of MetRSs be long ing to all king doms of life, as well as nu mer ous bio chem i cal and ge­netic stud ies have re vealed the pres ence of var i ous ad di tional do mains ap pended to the cat a lytic core of synthetase. They are re spon si ble for in ter ac tions with tRNA and pro teins. Ter tiary struc ture of C-terminal tRNA-binding ap pen di ces can be de duced from those de ter mined for their homo logues: tRNA bind ing pro tein 111 and en do the- lial monocyte-activating polypeptide II. Con tacts be tween MetRS and other pro teins could be me di ated not only by noncatalytic pep tides but also by struc tural el e ments pres ent in the cat a lytic core, e.g. Arg-Gly-Asp (RGD) mo tifs. Ad di tional ac tiv i ties in­volve MetRS in the main te nance of translational fi del ity and in co or di na tion of ri bo- some biogenesis with protein synthesis.
2

Inhibition of pea embryo axes protein biosynthesis by poly(A)-binding protein

86%
Sieliwanowicz B.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1987
|
tom 35
|
nr 1-3
3

Microscopic-electron examinations of the influence of the selected antibiotics on the secretory cells of the mammary gland

86%
Cegielski M.
Folia Histochemica et Cytobiologica
|
1986
|
tom 24
|
nr 4
4

The cell-free protein biosynthesis - applications and analysis of the system

86%
Lamla T., Mammeri K., Erdmann V. A.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 2
The in vitro protein biosynthesis has the potentials to be come a powerful technology for biochemical research. Beside the determination of structure and function the in vi­tro evolution of proteins is also of great interest. The system described was used to produce bo vine heart fatty acid binding protein (FABP) and bacterial chloramphenicol acetyltransferase (CAT) with and with out fu­sion of the Strep-tag II af fin ity pep tide. The proteins were purified after and during protein biosynthesis by using a StrepTactin Sepharose matrix. No significant influence of the Strep-tag and the conditions during the af fin ity chro ma tog ra phy on mat li­ra tion or activity of the protein was observed. The in vitro evolution of proteins is feasible by means of ribosome display. The selec­tion of a specific mRNA coding for a short ened FABP with a N-terminal His-tag via the accompanying protein property was shown. Goal of the selection was to bind the FABP via the His-tag on Ni(II)-IDA-agarose. Af ter nine cy cles of tran scrip tion, trans la tion, affinity selection and RT-PCR the protein with the His-tag could be enriched 108-fold. In or der to cor re late a pos si ble re la tion ship be tween changes in pro tein pop u la tion and bi o log i cal func tion stud ies were ini ti ated in which 2-dimensional pro tein pat terns of the to tal in vitro sys tem were com pared af ter 0 and 2 h re ac tion time. The very in ter- esting findings are that a number of proteins disappear, while others are newly formed dur ing protein synthesis.
5

Towards understanding of the role of transcription factors learning processes

86%
Kaczmarek L.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
Recent advances in application of molecular biology to studies on learning and memory formation suggest that understanding of these seemingly elusive phenomena may be within our reach. This mini-review summarizes the present knowledge on activation and possible functions of transcription factors in learning processes with a focus on studies performed in the author's laboratory.
6

Structure and function of intersubunit bridges in procaryotic ribosome

72%
Kietrys A. M., Szopa A., Bakowska-Zywicka K.
Biotechnologia
|
2009
|
nr 1
48-58
7

The structure of the ribosome - short history

72%
Bakowska-Zywicka K., Tyczewska A.
Biotechnologia
|
2009
|
nr 1
14-23
8

Recycling of eucaryotic ribosomes

72%
Tyczewska A., Bakowska-Zywicka K.
Biotechnologia
|
2009
|
nr 1
81-85
9

Structure and functions of 5S rRNA

72%
Barciszewska M. Z., Szymanski M., Erdmann V. A., Barciszewski J.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 1
The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyl- transferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.
10

Extraribosomal function of the acidic ribosomal P1-protein YP1alpha from Saccharomyces cerevisiae

72%
Tchorzewski M., Boldyreff B., Grankowski N.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 4
The yeast acidic ribosomal P-proteins YP1α, YP1β, YP2a and YP2b were studied for a possible transactivation potential beside their ribosomal function. The fusions of P-proteins with the GAL4 DNA-binding domain were assayed toward their transcriptional activity with the aid of reporter genes in yeast. Two of the P-proteins, YP1α and YP1β, exhibited transactivation potential, however, only YP1α can be regarded as a potent transactivator. This protein was able to transactivate a reporter gene associated with two distinct promoter systems, GAL1 or CYC1. Additionally, truncated proteins of YP1α and YP1β were analyzed. The N-terminal part of YP1α fused to GAL4-BD showed transactivation potential but the C-terminal part did not. Our results suggest a putative extraribosomal function for these ribosomal proteins which consequently may be classified as "moonlighting" proteins.
11

Structural basis for the antibacterial activity of the 12-membered-ring mono-sugar macrolide methymycin

58%
Auerbach T., Mermershtain I., Bashan A., Davidovich C., Rozenberg H., Sherman D. H., Yonath A.
Biotechnologia
|
2009
|
nr 1
24-35
12

Investigation of ribosomes of rat liver regenerating after partial hepatectomy using fluorescence, electron microscopy and biochemical analysis of isolated subcellular fractions

58%
Jaszczuk-Jarosz B., Turski W. A., Bartel H.
Folia Histochemica et Cytobiologica
|
1984
|
tom 22
|
nr 3-4
13

The new aspects of aminoacyl-tRNA synthetases

58%
Szymanski M., Deniziak M., Barciszewski J.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 3
Aminoacyl-tRNA synthetases (AARS) are essential proteins found in all living organisms. They form a diverse group of enzymes that ensure the fidelity of transfer of genetic information from the DNA into the protein. AARS catalyse the attachment of amino acids to transfer RNAs and thereby establish the rules of the genetic code by virtue of matching the nucleotide triplet of the anticodon with its cognate amino acid. Here we summarise the effects of recent studies on this interesting family of multifunctional enzymes.
14

Wpływ ciągłej, dożwaczowej infuzji mocznika na jego wykorzystanie w procesie syntezy białka mikroorganizmów w żwaczu

51%
Pisulewski P., Antoniewicz A., Filipczuk U.
Acta Agraria et Silvestria. Series Zootechnica
|
1988
|
tom 27
15

Glutamate receptor - driven gene expression in learning

51%
Kaczmarek L.
Acta Neurobiologiae Experimentalis
|
1993
|
tom 53
|
nr 1
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