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Characterization of proteins associating with 5' terminus of PGHS-1 mRNA

100%

Bunimov N., Laneuville O.

Cellular and Molecular Biology Letters

2010

tom 15

nr 2

Induction of Prostaglandin Endoperoxide H Synthase-1 (PGHS-1) gene has been previously documented in a few studies during events such as development and cellular differentiation. However, molecular mechanisms governing the regulation of PGHS-1 gene expression and contributing to changes in protein levels are poorly understood. Using the MEG-01 cell model of PGHS-1 gene induction, our laboratory has previously demonstrated that the 5’UTR and the first two exons of PGHS-1 mRNA had a significant impact on decreasing the translational efficiency of a reporter gene and suggested that the presence of a secondary structure is required for conservation of this activity. This 5’end of PGHS-1 mRNA sequence has also been shown to associate with nucleolin protein. In the current study, we set to investigate the protein composition of the mRNP (messenger ribonucleoprotein) associating with the 5’end of PGHS-1 mRNA and to identify its protein members. RNA/protein binding assays coupled with LC-MS analysis identified serpin B1 and NF45 (nuclear factor 45) proteins as potential members of PGHS-1 mRNP complex. Immunoprecipitation experiments using MEG-01 protein extracts validated mass spectrometry data and confirmed binding of nucleolin, serpin B1, NF45 and NF90. The RNA fraction was extracted from immunoprecipitated mRNP complexes and association of RNA binding proteins, serpin B1, NF45 and NF90, to PGHS-1 mRNA target sequence was confirmed by RT-PCR. Together these data suggest that serpin B1, NF45 and NF90 associate with PGHS-1 mRNA and can potentially participate in the formation a single or a number of PGHS-1 ribonucleoprotein complexes, through nucleolin that possibly serves as a docking base for other protein complex members.

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Differential inhibition of human prostaglandin endoperoxide synthase-1 and -2 by nonsteroidal anti-inflammatory drugs

67%

Patrignani P., Panara M. R., Sciulli M. G., Santini G., Renda G., Patrono C.

Journal of Physiology and Pharmacology

1997

tom 48

nr 4

Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa

59%

Mauro A., Lipari L., Leone A., Tortorici S., Burruano F., Provenzano S., Gerbino A., Buscemi M.

Folia Histochemica et Cytobiologica

2010

tom 48

nr 4

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Folia Histochemica et Cytobiologica

1 Journal of Physiology and Pharmacology

1 Bunimov N.

1 Burruano F.

1 Buscemi M.

1 Gerbino A.

1 Laneuville O.

1 Leone A.

1 Lipari L.

1 Mauro A.

1 Panara M. R.

1 Patrignani P.

1 Patrono C.

1 Provenzano S.

1 Renda G.

1 Santini G.

1 Sciulli M. G.

1 Tortorici S.

2 2010

1 1997

Wyniki wyszukiwania

Characterization of proteins associating with 5' terminus of PGHS-1 mRNA

Differential inhibition of human prostaglandin endoperoxide synthase-1 and -2 by nonsteroidal anti-inflammatory drugs

Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa