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Three seed priming techniques: hydropriming, halopriming and osmopriming, were compared for their effects on germination and vigour of pansy (Viola × wittrockiana Gams.) seeds at 20°C, 30°C and 35°C. Seeds were hydroprimed in the restricted volumes of water (600 and 700 μl H2O·g seed-1, 2, 3 or 4 days), haloprimed in KNO3 solution (-1.5 MPa, 5 days), and osmoprimed in polyethylene glycol solutions (-1.0, -1.25 or -1.5 MPa PEG 8000, 7 days) at 15°C or 20°C. Seed germination and vigour tests were performed for untreated and primed seeds. Generally, hydropriming negatively affected the speed of germination, the percentage of germinating seeds and germination capacity. Halopriming accelerated seed germination at 20°C, 30°C and 35°C but did not influence the percentage of germinating seeds and germination capacity. Osmopriming of seeds in PEG solution of osmotic potential –1.0 MPa at 20°C not only improved germination rates at 20°C, 30°C, and 35°C to the highest extent, but also increased percentage of germinating seeds at 30°C and 35°C most effectively and positively affected seed germination capacity at 20°C and 30°C.
Priming is a treatment applied for seed enhancement. Brassica vegetable seeds are commonly affected by Alternaria brassicicola. The changes in germination and vigour of kohlrabi (Brassica oleracea L. var. gongylodes L.) seeds subjected to two priming methods in the presence of A. brassicicola were investigated. The following treatments were applied: uninoculated non-primed seeds (control), seeds inoculated with A. brassicicola (2∙104 spores ml-1), uninoculated and inoculated seeds hydroprimed for 1–2 days, and uninoculated and inoculated seeds osmoprimed for 1–4 days. The percentage of germinating seeds, germination capacity, the percentages of diseased seedlings and dead seeds were determined. The speed of germination, tetrazolium staining index, and electrical conductivity described seed vigour. The presence of A. brassicicola on/in seeds was confirmed on CW semiselective agar. Osmopriming was conducive to penetration of A. brassicicola into kohlrabi seeds. The presence of the fungus had no influence on the percentage of germinating seeds, but a significantly lower germination capacity and a higher percentage of diseased seedlings and dead seeds were observed after inoculation. Non-primed inoculated seeds germinated faster than uninoculated ones. Despite the presence of A. brassicicola, osmotic priming improved seed vigour as measured by the speed of seed germination and electrical conductivity. The results of the tetrazolium vigour test showed its limited usefulness for seeds heavily infected with A. brassicicola.
The aim of this study was to evaluate the effect of pre-sowing seeds treatments on some agro-physiological parameters of sugar beets. The selected seeds treatments were: laser stimulation, hydropriming and combination of hydropriming and laser radiation. The impact of the seeds stimulation was analysed by determining the acid phosphatase activity, concentration of phosphate and photosynthetic pigments in leaves as well as nutrient status and some yield parameters of the roots. The plants were assayed four times during vegetation period. Experiment shown that different priming methods had relatively small effect on both the level of orthophosphate and total phosphorus concentration. On the other hand, the plants raised from seeds both hydro- and laser-primed remained more stable level of phosphate during whole vegetative season than remaining plants. The highest activity of acid phosphatase was observed in the younger plants and amongst different seed treatments, plants grown from non-primed seeds and laser primed seeds showed the highest enzyme activity. In the later stages of growth the activity considerably lowered. Considering the effect of different seeds treatments it was observed a significant increase in enzyme activity in plants emerged from the hydroprimed or laser stimulated seeds as well as an increment of chlorophylls in plants emerged from the hydroprimed seeds and both hydro- and laser primed ones. Seeds stimulation had a positive effect on the potassium content particularly in leaves of sugar beet and on some yield parameters of the roots. Concluding, it can be stated that the effect of pre-sowing seed stimulation (priming) was visible during the entire growing season and amongst the examined methods of seeds treatment, hydropriming sole or in a combination with laser radiation caused the highest alterations in assayed parameters.
Cytological changes in quiescent or germinated embryos after matriconditioning with Micro-Cel E or osmoconditioning with polyethylene glycol (PEG) were studied in comparison to quiescent or germinated untreated embryos of marjoram (Majorana hortensis L.). The sequence of changes related to embryo activation was identical in untreated and conditioned samples, although conditioned embryos underwent activation earlier. In those embryos the degradation of protein and lipid bodies, accompanied by vacuolation and accumulation of starch grains in amyloplasts, was observed even in nongerminated samples, whereas in control embryos the same ultrastructural changes did not occur until germination. The changes in ultrastructure occurred first in the root cap and proceeded towards the shoot meristem. In cotyledons, few symptoms of activation were detected regardless of the treatment. The appearance of Golgi structures in the root cap identified the radicle protrusion stage of germination.
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