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The present studies were carried out on the brains of the adult mole and rabbit. The preparations were made by means of the Golgi technique and the Nissl method. Two types of neurons were distinguished in the preoptic area (POA) of both species: bipolar and multipolar. The bipolar neurons have oval, fusiform or round perikarya and two dendritic trunks arising from the opposite poles of the cell body. The dendrites bifurcate once or twice. The dendritic branches have swellings, single spine-like and filiform processes. The multipolar neurons usually have triangular and quadrangular perikarya and from 3 to 5 dendritic trunks. The dendrites of the mole neurons branch sparsely, whereas the dendrites of the rabbit neurons display 2 or 3 divisions. On the dendritic branches varicosities and different protuberances were observed. The general morphology of the bipolar and multipolar neurons is similar in the mammals studied, although the neurons of the rabbit POA display a more complicated structure. Their dendritic branches show more divisions and possess more swellings and different processes than the dendrites of the neurons of the mole POA. Furthermore, of the multipolar neurons only the dendrites in POA of the rabbit were observed to have a rosary-like beaded appearance.
The aim of the study was to provide the topography and morphometric characteristics of the preoptic area (POA) of the guinea pig. The study was carried out on the brains of sexually mature guinea pigs of both sexes. A uniform procedure was followed in the study of the paraffin-embedded brain tissue blocks of males and females. The blocks were cut in the coronal plane into 50 μm sections and stained according to the Nissl method. The guinea pig POA consists of four parts: the medial preoptic area (MPA), lateral preoptic area (LPA), periventricular preoptic nucleus (PPN), and median preoptic nucleus (MPN). The topography and general structure of POA parts are similar in males and females. However, the PPNa cells of females are more intensely stained and are more densely packed than the PPNa cells of males. For morphometric analysis, the MPA and LPA as well as PPN and MPN were considered respectively as uniform structures, namely MPA-LPA and PPN-MPN. The statistical analysis showed that the volume of the PPN-MPN was larger in males than in females, whereas the MPA-LPA volume did not differ between the sexes. Moreover, the numerical density and the total number of neurons were statistically larger in males than in females in both the MPA-LPA and PPN-MPN. The parameters describing POA neurons were larger for MPA-LPA neurons in comparison with the PPN-MPN neurons. However, in this respect no sex differences were observed in both studied complexes. (Folia Morphol 2010; 69, 1: 15–23)
Orexin A and B are hypothalamic peptides involved in the control of food intake, sleep patterns, autonomic and neuroendocrine systems. The biological actions of orexins are mediated via two G-protein coupled receptors, termed orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). The present study analyzed mRNA and protein expressions of OX1R and OX2R in porcine hypothalamic structures engaged in GnRH production and secretion, preoptic area (POA), mediobasal hypothalamus (MBH), and stalk median eminence (SME) on days 2-3, 10-12, 14-16, and 17-19 of the oestrous cycle. The highest OX1R gene expression in POA was observed on days 17-19 of the cycle. Changes in the mRNA expression in MBH and SME throughout the cycle were negligible. The expression peak of OX2R gene in POA and SME occurred on days 17-19 as well. There were no changes in the gene expression in MBH. OX1R protein content in POA and SME also was the greatest on days 17-19 and OX2R protein expression was most pronounced in MBH and SME during the same phase of the cycle. In conclusion, fluctuation of OX1R and OX2R mRNAs and proteins content in pig hypothalamus dependently on the phase of the oestrous cycle suggests that orexins, through the influence on the hypothalamic-pituitary-ovarian axis, may affect reproductive functions.
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