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The aim of this study was to create new strategies to increase the pregnancy rate, in "ovsynch protocol" treatment. Two programs for synchronisation of ovulation and for synchronisation of ovulation and oestrus, similar to the ovsynch, were developed for the use in lactating primiparous dairy cows. Lactating Holstein-Friesian cows were randomly divided into five treatment groups: the GPG group (ovsynch) was treated with GnRH on day 0, PGF (PGF2α) on day 7, and received the second dose of GnRH 48 h later; the groups -7PGPG and - 2PGPG received the same treatment as the GPG group, but were given an additional injection of PGF 7 and 2 d before the start of the GPG treatment; respectively, the PG9PG group received the same treatment as the -2PGPG group, with the modification that the first GnRH injection was given simultaneously with the first PGF on the 2nd d; the GPEG group received the same treatment as the GPG group, but was injected an additional oestradiol propionate (EP) 24 h after the PGF. Plasma progesterone concentrations were determined at the days of the first hormone injection and the last PGF injection. Ovulation rates after the first GnRH and last PGF injections were calculated and presumptive sizes of the follicles on the last PGF injection day were determined in all the cows by rectal palpation. Cows detected to be at oestrus in 72 h after the last PGF injection was inseminated between the 8th and 12th h of their oestrus. Cows not detected to be in oestrus by 72 h after the last PGF received timed artificial insemination (TAI). While the ovulations mostly occurred in the GPG, GPEG, and -7PGPG groups at a period between the 48th and 96th h after the last PGF injection, the ovulations had shifted and occurred between 72 and 120 h, with 66.7% of all ovulations recorded between 72 and 96 h in the -2PGPG group. In the PG9PG group, ovulations took place dispersedly between the 0th and 120th h after the last PGF injection. The pre-synchronisation treatment (-7PGPG) by a PGF injection 7 d prior to the ovsynch protocol did not enhance the ovulation or pregnancy rates. The pre-synchronisation treatment by PGF injected 2 d before the ovsynch protocol, increased the number of cows that ovulated after the first GnRH injection (88.9% vs. 38.9%; P<0.05), produced smaller follicles at the time of the second PGF injection (1.1±0.13 vs. 1.6±0.16 cm; P<0.05) and increased pregnancy rates numerically (72.2% vs. 50.0%; P>0.05). Adding EP to GPG (GPEG), enhanced the expression of oestrus (P<0.05) and increased pregnancy rates slightly (60.0% vs. 50.0%; P>0.05). In conclusion, the -2PGPG and GPEG treatments are potentially new methods for routine synchronisation of ovulation and oestrus and/or ovulation, respectively, in primiparous Holstein-Friesian cows.
The study was carried out during April and May 2006, on thirty-eight 15 - 23-month-old Holstein heifers with inactive ovaries, which were selected from a private dairy herd with 300 heifers. The heifers were randomly distributed into two groups: norgestomet group (n=29), and a control group (n=9). In the norgestomet group, silicone ear implants containing 6 mg of norgestomet were implanted, and a solution containing 3 mg of norgestomet and 5 mg of oestradiol valerate was injected intramuscularly. The silicone implants were removed 11 d later, and the heifers were continuously observed for signs of oestrus for three days. The heifers were inseminated 48 and 78 h after the removal of the implants, and after the first insemination they were treated with 50 µg of GnRH analogue. The rate of the induced oestrus was 86.2% (25/29) and 0% (0/9) in norgestomet and control groups, respectively. The pregnancy rate was 48.2% (14/29) and 0% (0/9) in norgestomet and control groups, respectively. Our results showed that fertile oestrus can be stimulated successfully in post-pubertal heifers with inactive ovaries by a norgestomet treatment, and-successful pregnancy rate can be obtained by a fixed-time insemination during this oestrus.
Multiple ovulations and resulting multiple pregnancies leading to abortions or births of underdeveloped foals are important problems in Thoroughbred reproduction. The incidence of multiple ovulation during one ovarian cycle per mare in 44 Thoroughbred mares was determined by ultrasonic scanning. Twenty-two mares (50%) ovulated two (27.3%) or three (22.7%) dominant follicles during observed follicular phase. Overall 76 dominant follicles were ovulated, including 45 (59.2%) from right and 31 (40.8%) from left ovary. The mares at the age 6-10 years tended to have higher multiple ovulation rate (63.2°/0) than those below 6 or of more than 10 years old (both age groups amounting to 40%). Multiple ovulation rate was found lower in lactating than in non-lactating mares (-16.7 and 51.7%, respectively). No significant differences in the incidence of single vs multiple ovulation were found between the left and right ovary, age, or reproductive status of mares. The pregnancy on day 17 occurred in 7 (15.9%) single-ovulating mares. Twelve multiple-ovulating mares (27.3%) were diagnosed to be pregnant, including 7 (15%) with single and 5 (11.4%) with twin pregnancy. The pregnancy rate did not significantly differ between single- and multiple-ovulating mares.
A relatively high rate of multiple ovulation and resulting multiple pregnancies are one of main problems in Thoroughbred reproduction. The aim of the study was to examine the relationship between the intensity of oestrous behaviour and incidence of multiple (MO) vs single (SO) ovulations in 47 Thoroughbred mares. The frequency of five distinct receptive and 12 non-receptive behavioural traits was analysed. For quantitative analysis of sexual behaviour an 8-point behavioural score (BS) expressing increasing mare’s sexual receptivity was used. MO occurred in 48.9% of ovarian cycles observed. Neither frequencies of receptive behaviours, nor BS differed significantly between MO and SO mares. Overall pregnancy rate per one ovulatory cycle was estimated as 40.4%. Pregnancy rate was higher in MO mares (52.2%) as compared to SO mares (29.2%); however, the difference was not proved significant.
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