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Practical implications of inconsistent germination and viability results in testing stored Fagus sylvatica seeds

100%

Bezdeckova L., Prochazkova Z., Matejka K.

Dendrobiology

2014

tom 71

Germination and viability of stored European beech (Fagus sylvatica) seeds can vary depending upon the time when the tests are done during seed storage. To determine the possible sources of such variation the germination (GERM), germination rate expressed as mean germination time (MGT) and viability (VIAB) of six beechnut lots (three lots from two crop years) were determined monthly for one year using controlled laboratory conditions and standard tests. Higher GERM of some lots occurred when tests for stored seeds were carried out in spring and early autumn while other lots germinated better during summer tests. Similarly, different germination speed (dormancy release) and VIAB were observed in different months for different lots. However, no consistent seasonal fluctuation in GERM, MGT or VIAB of the beechnuts was observed in the tests. The reason for this fluctuation seems to be initial quality (germination and dormancy) of beechnut lots rather than any endogenous factors

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

100%

Chrobak D., Kizerwetter-Swida M., Rzewuska M., Binek M.

Polish Journal of Veterinary Sciences

2011

tom 14

nr 2

A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius- specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.

Ograniczanie wyników

1 Dendrobiology

1 Polish Journal of Veterinary Sciences

1 Bezdeckova L.

1 Binek M.

1 Chrobak D.

1 Kizerwetter-Swida M.

1 Matejka K.

1 Prochazkova Z.

1 Rzewuska M.

1 2014

1 2011

Wyniki wyszukiwania

Practical implications of inconsistent germination and viability results in testing stored Fagus sylvatica seeds

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications