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The aim of this study was to investigate the relationship between blood plasma levels of leptin, ghrelin and parameters of lipid metabolism during the peripartum period in mares. The investigation included 15 purebred Arabian broodmares 5 to 13-years-old and 6 pony mares from 9 to 16-years-of-age. The blood samples were collected two weeks before the term of anticipated foaling and on days 0, 3, 9, 15 and 22 post partum. The levels of leptin and ghrelin in the blood plasma were determined using RIA kits (Linco Research) and the concentration of triacylglycerols (TG), glycerol, free fatty acids (WKT), total cholesterol and HDL cholesterol were measured via enzymatic methods. The plasma concentration of leptin, WKT, total cholesterol and HDL cholesterol attained maximum values at parturition day. Plasma TG and LDL cholesterol levels were the highest in late pregnancy and diminished in the days following parturition. The values of glycerol, WKT, total cholesterol and HDL cholesterol were lower and the levels of leptin and ghrelin were higher in purebred Arabians than in pony mares. A negative correlation was found between the plasma leptin concentration and the WKT/TG ratio. Leptin can play an important role in the regulation of lipid metabolism during the periparturient period in mares.
The experiments were carried out on a group of 90 males of guinea pigs of 300 ± 50 g in weight. The animals were divided into 6 groups equal in numbers. 5 of them were infected per os with ca 5000 invasive eggs of Ascaris suum. The blood of both infected and control guinea pigs was controlled on 1, 2, 4, 7, and 20th day after infection. The level of total lipids (according to Swahn method), WKT (Dole titrimetric method), total cholesterol and HDL cholesterol (Błaszczyszyn method) and triglicerides (colometric method) was determined. During the invasion, the decrease in all studied indicators was observed, and was particularly apparent on 4th and 7th day after infection. Only in case of WKT and triglicerides a slight increase at initial stage of invasion was found. During larval ascariasis, as it appears, the metabolism of lipids is significantly disturbed. The changes are due to the break in liver function and, presumedly, changes in hormon secrection which are provoked by the presence of the parasite.
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